Detailed view for TGME49_232300

Basic information

TDR Targets ID: 265092
Toxoplasma gondii, ribosomal protein RPS3
Source Database / ID:  ToxoDB 

pI: 10.1287 | Length (AA): 255 | MW (Da): 28737 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00189   Ribosomal protein S3, C-terminal domain
PF07650   KH domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0015935   small ribosomal subunit  
GO:0005840   ribosome  
GO:0003735   structural constituent of ribosome  
GO:0003723   RNA binding  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
26 249 4kzy (D) 4 227 72.00 0 1 1.69093 -0.01

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 5XXU:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_126820)

Species Accession Gene Product
Arabidopsis thaliana AT5G35530   40S ribosomal protein S3-3
Arabidopsis thaliana ArthCp061   30S ribosomal protein S3
Arabidopsis thaliana AT2G31610   40S ribosomal protein S3-1
Arabidopsis thaliana AT3G53870   40S ribosomal protein S3-2
Babesia bovis BBOV_II001650   40S ribosomal protein S3 protein, putative
Brugia malayi Bm1_11880   40S ribosomal protein S3
Candida albicans CaO19.6312   likely cytosolic ribosomal protein similar to S. cerevisiae RPS3 (YNL178W) small subunit protein S3
Candida albicans CaO19.13689   likely cytosolic ribosomal protein similar to S. cerevisiae RPS3 (YNL178W) small subunit protein S3
Caenorhabditis elegans CELE_C23G10.3   Protein RPS-3
Cryptosporidium hominis Chro.70257   ribosomal protein
Cryptosporidium parvum cgd7_2250   40S ribosomal protein S3, KH domain
Chlamydia trachomatis CT_522   30S ribosomal protein S3
Dictyostelium discoideum DDB_G0293000   40S ribosomal protein S3
Drosophila melanogaster Dmel_CG6779   Ribosomal protein S3
Escherichia coli b3314   30S ribosomal subunit protein S3
Echinococcus granulosus EgrG_001167300   ribosomal protein S3
Entamoeba histolytica EHI_148320   40S ribosomal protein S3, putative
Entamoeba histolytica EHI_146340   40S ribosomal protein S3, putative
Echinococcus multilocularis EmuJ_001167300   ribosomal protein S3
Giardia lamblia GL50803_7999   Ribosomal protein S3
Homo sapiens ENSG00000149273   ribosomal protein S3
Leishmania braziliensis LbrM.33.1110   40S ribosomal protein S3, putative
Leishmania braziliensis LbrM.15.0990   40S ribosomal protein S3, putative
Leishmania donovani LdBPK_330960.1   40S ribosomal protein S3, putative
Leishmania infantum LinJ.15.1010   40S ribosomal protein S3, putative
Leishmania infantum LinJ.33.0960   40S ribosomal protein S3, putative
Leishmania major LmjF.33.0920   40S ribosomal protein S3, putative
Leishmania major LmjF.15.0950   40S ribosomal protein S3, putative
Leishmania mexicana LmxM.15.0950   40S ribosomal protein S3, putative
Leishmania mexicana LmxM.32.0920   40S ribosomal protein S3, putative
Loa Loa (eye worm) LOAG_02954   40S ribosomal protein S3
Mycobacterium leprae ML1857c   PROBABLE 30S RIBOSOMAL PROTEIN S3 RPSC
Mus musculus ENSMUSG00000030744   ribosomal protein S3
Mycobacterium tuberculosis Rv0707   30S ribosomal protein S3 RpsC
Mycobacterium ulcerans MUL_0796   30S ribosomal protein S3
Neospora caninum NCLIV_032290   SSU ribosomal protein S3P, related
Oryza sativa 3131443   30S ribosomal protein S3
Oryza sativa 4333308   Os03g0577000
Oryza sativa 4343869   Os07g0608500
Onchocerca volvulus OVOC8169  
Plasmodium berghei PBANKA_1329300   40S ribosomal protein S3, putative
Plasmodium falciparum PF3D7_1465900   40S ribosomal protein S3
Plasmodium knowlesi PKNH_1215600   40S ribosomal protein S3, putative
Plasmodium vivax PVX_117170   40S ribosomal protein S3, putative
Saccharomyces cerevisiae YNL178W   ribosomal 40S subunit protein S3
Schistosoma japonicum Sjp_0207020   ko:K02985 small subunit ribosomal protein S3e, putative
Schistosoma mansoni Smp_027610   40S ribosomal protein S3
Schmidtea mediterranea mk4.013843.00  
Schmidtea mediterranea mk4.011171.01  
Schmidtea mediterranea mk4.003380.03   40S ribosomal protein S3
Trypanosoma brucei gambiense Tbg972.9.3160   40S ribosomal protein S3, putative
Trypanosoma brucei gambiense Tbg972.10.13910   40S ribosomal protein S3, putative
Trypanosoma brucei Tb927.10.11540   40S ribosomal protein S3, putative
Trypanosoma brucei Tb927.9.6070   40S ribosomal protein S3, putative
Trypanosoma congolense TcIL3000_10_9750   40S ribosomal protein S3, putative
Trypanosoma congolense TcIL3000_9_2070   40S ribosomal protein S3, putative
Trypanosoma cruzi TcCLB.509353.30   40S ribosomal protein S3, putative
Trypanosoma cruzi TcCLB.507677.39   40S ribosomal protein S3, putative
Trypanosoma cruzi TcCLB.430605.29   40S ribosomal protein S3, putative
Trypanosoma cruzi TcCLB.504021.109   40S ribosomal protein S3, putative
Toxoplasma gondii TGME49_232300   ribosomal protein RPS3
Treponema pallidum TP0195   30S ribosomal protein S3
Theileria parva TP04_0421   40S ribosomal protein S3, putative
Trichomonas vaginalis TVAG_420140   40S ribosomal protein S3, putative
Trichomonas vaginalis TVAG_106800   30S ribosomal protein S3, putative
Trichomonas vaginalis TVAG_462690   30S ribosomal protein S3, putative
Trichomonas vaginalis TVAG_314710   30S ribosomal protein S3, putative
Trichomonas vaginalis TVAG_263040   40S ribosomal protein S3, putative
Trichomonas vaginalis TVAG_178710   30S ribosomal protein S3, putative
Wolbachia endosymbiont of Brugia malayi Wbm0335   30S ribosomal protein S3

Essentiality

TGME49_232300 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.4450 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.4450 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.4450 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.4450 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.11540 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.11540 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.11540 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.11540 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3314 Escherichia coli essential goodall
CELE_C23G10.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_C23G10.3 Caenorhabditis elegans larval arrest wormbase
CELE_C23G10.3 Caenorhabditis elegans larval lethal wormbase
CELE_C23G10.3 Caenorhabditis elegans slow growth wormbase
CELE_C23G10.3 Caenorhabditis elegans sterile wormbase
YNL178W Saccharomyces cerevisiae inviable yeastgenome
TGME49_232300 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_232300 (Toxoplasma gondii), ribosomal protein RPS3
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