Detailed view for PVX_115075

Basic information

TDR Targets ID: 266170
Plasmodium vivax, calpain, putative

Source Database / ID:  PlasmoDB 

pI: 9.9856 | Length (AA): 2131 | MW (Da): 243189 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Pfam domains

PF00648   Calpain family cysteine protease
PF01067   Calpain large subunit, domain III

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0004198   calcium-dependent cysteine-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1483 1605 1ziv (A) 215 334 33.00 0.00000016 1 0.25 -0.27
1522 1605 2g8j (A) 268 351 37.00 0.0000012 0.46 0.35 -0.29
1959 2038 1zmz (A) 19 98 8.00 0.00000000099 0 0.16 -0.8
294 517 5cwp (A) 2 227 12.00 0.28 0.08 0.336315 -1.51
1020 1200 1ziv (A) 42 211 24.00 0 1 0.298837 -0.14
1483 1605 2p0r (A) 215 334 33.00 0.00000049 1 0.253119 -0.25
1483 1605 2nqa (A) 223 341 34.00 0.0000026 0.69 0.320119 -0.03

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intraerythrocytic - 40 hs, intraerythrocytic - 48 hs. Zhu L
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intraerythrocytic - 6 hs, intraerythrocytic - 12 hs, intraerythrocytic - 18 hs, intraerythrocytic - 24 hs, intraerythrocytic - 30 hs, intraerythrocytic - 36 hs. Zhu L
Show/Hide expression data references
  • Zhu L New insights into the Plasmodium vivax transcriptome using RNA-Seq.

Orthologs

Ortholog group members (OG5_128932)

Species Accession Gene Product
Babesia bovis BBOV_III007670   calpain family cysteine protease domain containing protein
Brugia malayi Bm1_14050   calpain 7
Candida albicans CaO19.3995   similar to Aspergillus calpain-like protease PalBory involved in sporulation
Candida albicans CaO19.11478   similar to Aspergillus calpain-like protease PalBory involved in sporulation
Caenorhabditis elegans CELE_T04A8.16   Protein CLP-2
Cryptosporidium hominis Chro.80156   hypothetical protein
Cryptosporidium parvum cgd8_1320   calpain like thiol protease
Echinococcus granulosus EgrG_001000650   calpain 7 C02 family
Echinococcus multilocularis EmuJ_001000650   calpain 7 (C02 family)
Homo sapiens ENSG00000131375   calpain 7
Leishmania braziliensis LbrM.33.2290   calpain protease-like protein
Leishmania donovani LdBPK_332130.1   calpain protease-like protein
Leishmania infantum LinJ.33.2130   calpain protease-like protein
Leishmania major LmjF.33.2010   calpain protease-like protein
Leishmania mexicana LmxM.32.2010   calpain protease-like protein
Loa Loa (eye worm) LOAG_11530   calpain
Mus musculus ENSMUSG00000021893   calpain 7
Neospora caninum NCLIV_000790   hypothetical protein
Plasmodium berghei PBANKA_1138400   calpain, putative
Plasmodium falciparum PF3D7_1362400   calpain
Plasmodium knowlesi PKNH_1108900   calpain, putative
Plasmodium vivax PVX_115075 this record   calpain, putative
Plasmodium yoelii PY00976   Capn7-related
Saccharomyces cerevisiae YMR154C   Rim13p
Schistosoma japonicum Sjp_0206230   Probable ubiquitin carboxyl-terminal hydrolase R10E11.3, putative
Schistosoma japonicum Sjp_0035290   ko:K08576 calpain-7, putative
Schistosoma mansoni Smp_083530.1   calpain-7 (C02 family)
Schistosoma mansoni Smp_083530.2   calpain-7 (C02 family)
Schmidtea mediterranea mk4.007205.03  
Schmidtea mediterranea mk4.015113.02  
Schmidtea mediterranea mk4.008769.01   Calpain-7
Schmidtea mediterranea mk4.015113.00   Calpain-7
Trypanosoma brucei gambiense Tbg972.11.5280   calpain-like protein, putative
Trypanosoma brucei Tb927.11.4640   calpain-like protein, putative
Trypanosoma brucei Tb427tmp.02.2240   calpain-like protein, putative
Trypanosoma congolense TcIL3000.11.4720   calpain-like protein, putative
Trypanosoma cruzi TcCLB.511847.10   calpain-like cysteine peptidase, putative
Trypanosoma cruzi TcCLB.506227.130   calpain-like cysteine peptidase, putative
Toxoplasma gondii TGME49_293820   calpain family cysteine protease domain-containing protein
Theileria parva TP04_0664   hypothetical protein

Essentiality

PVX_115075 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.2240 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.2240 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.2240 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.2240 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1138400 Plasmodium berghei Essential plasmo
TGME49_293820 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
 
In any case, if you have information about papers containing relevant validation data for this target, please log in using your TDR Targets username and password and send them to us using the corresponding form in this page (only visible to registered users) or contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania major calpain protease-like protein Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0223 0.2855 1
0.0389 0.3414 1
0.0283 0.3069 1
0.0298 0.2723 1
0.142 0.2741 0.8976
0.0946 0.2967 1
0.046 0.3127 0.6705
0.0868 0.2713 0.7779
0.257 0.2886 1
0.0418 0.2828 1
0.0215 0.2552 0.5
0.0586 0.2951 0.6942
0.0338 0.2921 1
0.0296 0.3365 0.8781
0.121 0.2735 0.8763
0.344 0.2792 1
0.1243 0.2529 0.8003
0.0501 0.3381 0.698
0.1061 0.2831 0.5
0.0353 0.3411 0.5516
0.1287 0.2885 1
0.0286 0.3189 0.5564
0.0257 0.2895 0.9002
0.0614 0.4376 0.8575

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PVX_115075 (Plasmodium vivax), calpain, putative
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