Detailed view for LmjF.27.1780
Basic information
Leishmania major, casein kinase I-like protein
Source Database / ID: TriTrypDB GeneDB
pI: 7.1595 |
Length (AA): 560 |
MW (Da): 61293 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Network
Pfam domains
Gene Ontology
GO:0004713 protein tyrosine kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004672 protein kinase activity
GO:0006468 protein amino acid phosphorylation
Metabolic Pathways
Structural information
Modbase 3D models:
There are 8 models calculated for this protein. More info on
these models, including the
models themselves is available at:
Modbase
| Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
|---|---|---|---|---|---|---|---|---|---|
| 87 | 473 | 2bdw (A) | 5 | 315 | 19.00 | 0 | 1 | 0.53 | 0.33 |
| 187 | 407 | 1csn () | 47 | 277 | 39.00 | 0 | 1 | 0.94 | -1.04 |
| 9 | 91 | 2lvf (A) | 16 | 96 | 14.00 | 0 | 0.02 | 0.286414 | -0.52 |
| 39 | 418 | 4g3f (A) | 346 | 660 | 21.00 | 0.0000000026 | 0.99 | 0.504871 | 0.66 |
| 97 | 451 | 1fot (A) | 86 | 361 | 21.00 | 0 | 1 | 0.480229 | 0.67 |
| 222 | 378 | 4g3c (B) | 474 | 635 | 27.00 | 0.000015 | 1 | 0.649257 | -0.83 |
| 248 | 292 | 3niz (A) | 127 | 169 | 44.00 | 0.12 | 0.95 | 0.564257 | -0.23 |
| 249 | 287 | 2buj (A) | 132 | 168 | 57.00 | 0.011 | 1 | 0.672543 | -0.17 |
Help me make sense of these data.
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
Expression
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Upper 60-80% percentile | metacyclic. | Fernandes MC |
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Mid 40-60% percentile | amastigotes. | Fernandes MC |
-
Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.
Orthologs
Ortholog group members (OG5_183743)
| Species | Accession | Gene Product |
|---|---|---|
| Leishmania braziliensis | LbrM.27.1900 | casein kinase I-like protein |
| Leishmania donovani | LdBPK_271680.1 | casein kinase I-like protein |
| Leishmania infantum | LinJ.27.1680 | casein kinase I-like protein |
| Leishmania major | LmjF.27.1780 | casein kinase I-like protein |
| Leishmania mexicana | LmxM.27.1780 | casein kinase I-like protein |
Essentiality
Phenotypes and Validation (curated)
Annotated phenotypes:
| Affected Entity | Phenotypic quality | Occurs in | Occurs at | Evidence | Observed in | Drugs/Inhibitors |
|---|---|---|---|---|---|---|
| catalytic activity (GO:0003824) | decreased (PATO:0000468) | in vitro (MI:0492) | inferred from specific protein inhibition (ECO:0000020) | Leishmania major | No drug identifiers listed for this gene. | |
| Annotator: | crowther@u.washington.edu. | Comment: | Drug: 188343-77-3; Drug: 191030-70-3; Drug: 762172-81-6; Drug: 480453-85-8. chemical inhibitors reduced casein kinase activity and growth of promastigotes. | References: | 16890941 | |
| growth (GO:0040007) | decreased time (PATO:0000716) | single cell organism (CARO:0000064) | promastigote (BTO:0001124) | inferred from bioassay (ECO:0000094) | Leishmania major | No drug identifiers listed for this gene. |
| Annotator: | crowther@u.washington.edu. | Comment: | Drug: 188343-77-3; Drug: 191030-70-3; Drug: 762172-81-6; Drug: 480453-85-8. chemical inhibitors reduced casein kinase activity and growth of promastigotes. | References: | 16890941 | |
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Annotated validation
Associated compounds / Druggability
Druggability index (range: 0 to 1): 0.4
Known modulators for this target
Predicted associations
By orthology with druggable targets
By sequence similarity to non orthologous druggable targets
| Species | Target | Length | Identity | Alignment span | Linked Drugs | Reference |
|---|---|---|---|---|---|---|
| Bos taurus | Casein kinase I isoform alpha | 325 aa | 31.2% | 324 aa | Compounds | References |
Obtained from network model
Ranking Plot
Putative Drugs List
Assayability
Assay information
Reagent availability
Bibliographic References