Detailed view for PVX_119720

Basic information

TDR Targets ID: 270301
Plasmodium vivax, 26S proteasome regulatory subunit RPN12, putative
Source Database / ID:  PlasmoDB 

pI: 4.9489 | Length (AA): 303 | MW (Da): 34629 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF10075   CSN8/PSMD8/EIF3K family

Gene Ontology

Mouse over links to read term descriptions.
GO:0005838   proteasome regulatory particle  
GO:0006508   proteolysis  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
53 303 1rz4 (A) 9 214 16.00 0 0.8 0.73 -0.58
27 274 4b0z (A) 0 222 29.00 0 1 1.03568 -0.19
35 302 3t5v (A) 263 539 15.00 0 0.94 0.890688 0.26

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intraerythrocytic - 6 hs, intraerythrocytic - 12 hs, intraerythrocytic - 18 hs, intraerythrocytic - 24 hs, intraerythrocytic - 30 hs, intraerythrocytic - 36 hs. Zhu L
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intraerythrocytic - 40 hs. Zhu L
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intraerythrocytic - 48 hs. Zhu L
Show/Hide expression data references
  • Zhu L New insights into the Plasmodium vivax transcriptome using RNA-Seq.

Orthologs

Ortholog group members (OG5_128073)

Species Accession Gene Product
Arabidopsis thaliana AT5G42040   26S proteasome non-ATPase regulatory subunit RPN12B
Arabidopsis thaliana AT1G64520   26S proteasome non-ATPase regulatory subunit RPN12A
Babesia bovis BBOV_III003610   26S proteasome non-ATPase regulatory subunit Nin1/mts3 family protein
Brugia malayi Bm1_45690   26S proteasome non-ATPase regulatory subunit Nin1/mts3 family protein
Brugia malayi Bm1_44745   26S proteasome non-ATPase regulatory subunit Nin1/mts3 family protein
Candida albicans CaO19.7845   similar to S. cerevisiae RPN12 (YFR052W) subunit of the 19S regulatory particle of the 26S proteasome
Candida albicans CaO19.213   similar to S. cerevisiae RPN12 (YFR052W) subunit of the 19S regulatory particle of the 26S proteasome
Caenorhabditis elegans CELE_ZK20.5   Protein RPN-12
Cryptosporidium hominis Chro.20354   hypothetical protein
Cryptosporidium parvum cgd2_3370   proteasome regulatory subunit Rpn12 family
Dictyostelium discoideum DDB_G0273979   26S proteasome non-ATPase regulatory subunit 8
Dictyostelium discoideum DDB_G0272564   26S proteasome non-ATPase regulatory subunit 8
Drosophila melanogaster Dmel_CG11552   Regulatory particle non-ATPase 12-related
Drosophila melanogaster Dmel_CG4157   Regulatory particle non-ATPase 12
Echinococcus granulosus EgrG_000795500   26S proteasome non ATPase regulatory subunit 8
Entamoeba histolytica EHI_200220   proteasome regulatory subunit, putative
Echinococcus multilocularis EmuJ_000795500   26S proteasome non ATPase regulatory subunit 8
Homo sapiens ENSG00000099341   proteasome (prosome, macropain) 26S subunit, non-ATPase, 8
Leishmania braziliensis LbrM.32.1350   proteasome regulatory non-ATP-ase subunit, putative
Leishmania donovani LdBPK_321260.1   proteasome regulatory non-ATP-ase subunit, putative
Leishmania infantum LinJ.32.1260   proteasome regulatory non-ATP-ase subunit, putative
Leishmania major LmjF.32.1200   proteasome regulatory non-ATP-ase subunit, putative
Leishmania mexicana LmxM.31.1200   proteasome regulatory non-ATP-ase subunit, putative
Loa Loa (eye worm) LOAG_02141   hypothetical protein
Loa Loa (eye worm) LOAG_08779   hypothetical protein
Mus musculus ENSMUSG00000030591   proteasome (prosome, macropain) 26S subunit, non-ATPase, 8
Neospora caninum NCLIV_066370   hypothetical protein
Oryza sativa 4343069   Os07g0435100
Plasmodium berghei PBANKA_0410300   26S proteasome regulatory subunit RPN12, putative
Plasmodium falciparum PF3D7_0312300   26S proteasome regulatory subunit RPN12, putative
Plasmodium knowlesi PKNH_0830100   26S proteasome regulatory subunit RPN12, putative
Plasmodium vivax PVX_119720   26S proteasome regulatory subunit RPN12, putative
Plasmodium yoelii PY01718   hypothetical protein
Saccharomyces cerevisiae YFR052W   proteasome regulatory particle lid subunit RPN12
Schistosoma japonicum Sjp_0024600   ko:K03031 26S proteasome regulatory subunit N12, putative
Schistosoma mansoni Smp_047740   26S proteasome non-ATPase regulatory subunit
Schmidtea mediterranea mk4.003873.04   26S proteasome non-ATPase regulatory subunit 8
Schmidtea mediterranea mk4.001636.00   26S proteasome non-ATPase regulatory subunit 8
Schmidtea mediterranea mk4.000673.02   26S proteasome non-ATPase regulatory subunit 8
Schmidtea mediterranea mk4.012294.00   26S proteasome non-ATPase regulatory subunit 8
Schmidtea mediterranea mk4.025000.00   26S proteasome non-ATPase regulatory subunit 8
Trypanosoma brucei gambiense Tbg972.11.16170   proteasome regulatory non-ATP-ase subunit, putative
Trypanosoma brucei Tb927.11.14430   proteasome regulatory non-ATP-ase subunit
Trypanosoma congolense TcIL3000.11.14720   proteasome regulatory non-ATP-ase subunit
Trypanosoma cruzi TcCLB.511725.10   proteasome regulatory non-ATPase subunit, putative
Toxoplasma gondii TGME49_250830   SAC3/GANP family protein
Theileria parva TP03_0046   26S proteasome regulatory subunit S14, putative
Trichomonas vaginalis TVAG_321570   26S proteasome non-ATPase regulatory subunit, putative
Trichomonas vaginalis TVAG_171960   26S proteasome non-ATPase regulatory subunit, putative
Trichomonas vaginalis TVAG_253730   26S proteasome non-ATPase regulatory subunit, putative

Essentiality

PVX_119720 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.6030 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.6030 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.6030 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.6030 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YFR052W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0410300 Plasmodium berghei Slow plasmo
TGME49_250830 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PVX_119720 (Plasmodium vivax), 26S proteasome regulatory subunit RPN12, putative
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