Detailed view for PF3D7_1440200

Basic information

TDR Targets ID: 2713
Plasmodium falciparum, stromal-processing peptidase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.9505 | Length (AA): 1560 | MW (Da): 185590 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00675   Insulinase (Peptidase family M16)
PF05193   Peptidase M16 inactive domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0020011   apicoplast  
GO:0046872   metal ion binding  
GO:0008270   zinc ion binding  
GO:0004655   porphobilinogen synthase activity  
GO:0004222   metalloendopeptidase activity  
GO:0003824   catalytic activity  
GO:0033014   tetrapyrrole biosynthetic process  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
50 1187 1q2l (A) 31 960 9.00 0 1 0.29 1.32
980 1237 1ezv (B) 31 285 10.00 0 0.85 0.34 -0.56
101 320 1hr6 (B) 27 208 21.00 0.00048 0.95 0.0416256 0.71
128 1285 3ffz (A) 159 1174 23.00 0.14 1 0.616308 1.75
129 263 2a06 (A) 41 185 28.00 0.32 0.23 0.125538 0.33
308 1111 3vuo (A) 361 1189 22.00 0.036 0.98 0.467385 1.85
356 706 4hte (A) 260 593 28.00 0.73 0.45 0.047 1.42
558 771 4tql (A) 21 237 12.00 0.24 0.29 0.330779 -0.98
786 1020 4u0r (A) 183 498 19.00 0.055 0.5 0.234241 0.13

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, early trophozoite, Oocyst. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, late trophozoite, Ring, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Sporozoite, Female Gametocyte. Zanghi G Lasonder E
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_135834)

Species Accession Gene Product
Arabidopsis thaliana AT5G42390   Insulinase (peptidase family M16) family protein
Babesia bovis BBOV_II001130   hypothetical protein
Cryptosporidium hominis Chro.20292   hypothetical protein
Cryptosporidium parvum cgd2_2760   peptidaseinsulinase-like peptidase
Neospora caninum NCLIV_050050   M16 family peptidase, putative
Neospora caninum NCLIV_008220   M16 family peptidase, putative
Oryza sativa 4341569   Os06g0625400
Plasmodium berghei PBANKA_1304200   stromal-processing peptidase, putative
Plasmodium falciparum PF3D7_1440200   stromal-processing peptidase, putative
Plasmodium knowlesi PKNH_1242000   stromal-processing peptidase, putative
Plasmodium vivax PVX_118475   stromal-processing peptidase, putative
Toxoplasma gondii TGME49_235680   peptidase M16 inactive domain-containing protein
Toxoplasma gondii TGME49_253890   peptidase M16 inactive domain-containing protein
Theileria parva TP04_0473   stromal processing peptidase precursor, putative

Essentiality

PF3D7_1440200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1304200 Plasmodium berghei Essential plasmo
TGME49_235680 Toxoplasma gondii Probably non-essential sidik
TGME49_253890 Toxoplasma gondii Probably non-essential sidik
TGME49_235680 Toxoplasma gondii Probably essential sidik
TGME49_253890 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1440200 (Plasmodium falciparum), stromal-processing peptidase, putative
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