Detailed view for LmjF.33.0830

Basic information

TDR Targets ID: 27534
Leishmania major, 2,4-dienoyl-coa reductase fadh1, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.4676 | Length (AA): 730 | MW (Da): 78693 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00724   NADH:flavin oxidoreductase / NADH oxidase family
PF07992   Pyridine nucleotide-disulphide oxidoreductase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0010181   FMN binding  
GO:0009055   electron carrier activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  
GO:0055114   oxidation reduction  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 730 1o94 (A) 4 695 20.00 0 1 0.88 -0.06
3 730 1ps9 (A) 1 671 43.00 0 1 1.28 -0.68
6 351 1z41 (A) 4 325 33.00 0 1 0.93 -1.39
3 730 1ps9 (A) 1 671 40.00 0 1 1.37576 0.15
4 730 1ps9 (A) 2 671 48.00 0 1 1.50229 -0.27
6 351 1z41 (A) 4 325 33.00 0 1 0.911373 -1.08
395 429 3dme (A) 7 41 40.00 0 0.41 0.432945 0.12

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127243)

Species Accession Gene Product
Arabidopsis thaliana AT1G09400   putative 12-oxophytodienoate reductase-like protein 1
Candida albicans CaO19.3131   potential NADH-dependent flavin oxidoreductase
Candida albicans CaO19.10643   potential NADH-dependent flavin oxidoreductase
Caenorhabditis elegans CELE_W06H8.2   Protein W06H8.2
Dictyostelium discoideum DDB_G0286923   NADH:flavin oxidoreductase/NADH oxidase domain-containing protein
Dictyostelium discoideum DDB_G0293114   NADH:flavin oxidoreductase/NADH oxidase domain-containing protein
Dictyostelium discoideum DDB_G0287765   NADH:flavin oxidoreductase/NADH oxidase domain-containing protein
Dictyostelium discoideum DDB_G0271752   NADH:flavin oxidoreductase/NADH oxidase domain-containing protein
Dictyostelium discoideum DDB_G0277969   NADH:flavin oxidoreductase/NADH oxidase domain-containing protein
Dictyostelium discoideum DDB_G0287561   NADH:flavin oxidoreductase/NADH oxidase domain-containing protein
Dictyostelium discoideum DDB_G0279473   NADH:flavin oxidoreductase/NADH oxidase domain-containing protein
Escherichia coli b3081   2,4-dienoyl-CoA reductase, NADH and FMN-linked
Giardia lamblia GL50803_9719   NADH oxidase
Leishmania braziliensis LbrM.33.1020   2,4-dienoyl-coa reductase fadh1, putative
Leishmania braziliensis LbrM.06.0910   2,4-dienoyl-coa reductase-like protein
Leishmania donovani LdBPK_330870.1   2,4-dienoyl-coa reductase fadh1, putative
Leishmania donovani LdBPK_060960.1   2,4-dienoyl-coa reductase-like protein
Leishmania infantum LinJ.06.0960   2,4-dienoyl-coa reductase-like protein
Leishmania infantum LinJ.33.0870   2,4-dienoyl-coa reductase fadh1, putative
Leishmania major LmjF.06.0930   2,4-dienoyl-coa reductase-like protein
Leishmania major LmjF.33.0830   2,4-dienoyl-coa reductase fadh1, putative
Leishmania mexicana LmxM.32.0830   2,4-dienoyl-coa reductase fadh1, putative
Leishmania mexicana LmxM.06.0930   2,4-dienoyl-coa reductase-like protein
Mycobacterium tuberculosis Rv1175c   Probable NADPH dependent 2,4-dienoyl-CoA reductase FadH (2,4-dienoyl coenzyme A reductase) (4-enoyl-CoA reductase)
Mycobacterium ulcerans MUL_0469   hypothetical protein
Mycobacterium ulcerans MUL_1023   NADPH dependent 2,4-dienoyl-CoA reductase FadH
Trypanosoma brucei gambiense Tbg972.7.6430   2,4-dienoyl-coa reductase-like protein
Trypanosoma brucei Tb927.7.5540   2,4-dienoyl-coa reductase-like protein
Trypanosoma congolense TcIL3000_7_4560   2,4-dienoyl-coa reductase-like protein, putative
Trypanosoma cruzi TcCLB.509941.100   2,4-dienoyl-coa reductase fadh1, putative
Trypanosoma cruzi TcCLB.510303.210   2,4-dienoyl-coa reductase-like protein, putative
Trichomonas vaginalis TVAG_220000   NADPH dehydrogenase, putative
Trichomonas vaginalis TVAG_152690   N-ethylmaleimide reductase, putative
Trichomonas vaginalis TVAG_443770   N-ethylmaleimide reductase, putative
Trichomonas vaginalis TVAG_405820   NADH:flavin oxidoreductase, putative
Trichomonas vaginalis TVAG_351540   2,4-dienoyl-CoA reductase [NADPH], putative
Trichomonas vaginalis TVAG_478830   N-ethylmaleimide reductase, putative
Trichomonas vaginalis TVAG_411220   N-ethylmaleimide reductase, putative
Trichomonas vaginalis TVAG_510340   N-ethylmaleimide reductase, putative
Trichomonas vaginalis TVAG_093680   2,4-dienoyl-CoA reductase [NADPH], putative
Trichomonas vaginalis TVAG_272880   NADH oxidase, putative
Trichomonas vaginalis TVAG_399520   2,4-dienoyl-CoA reductase [NADPH], putative
Trichomonas vaginalis TVAG_127520   N-ethylmaleimide reductase, putative
Trichomonas vaginalis TVAG_121620   N-ethylmaleimide reductase, putative

Essentiality

LmjF.33.0830 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1196 Mycobacterium tuberculosis non-essential nmpdr
Tb927.7.5540 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.5540 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.5540 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.5540 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3081 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.33.0830 (Leishmania major), 2,4-dienoyl-coa reductase fadh1, putative
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