Detailed view for LmjF.32.1810

Basic information

TDR Targets ID: 27633
Leishmania major, protein kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.5546 | Length (AA): 655 | MW (Da): 70536 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00169   PH domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
12 463 1opk (A) 130 508 19.00 0 1 0.65 0.61
62 651 1omw (A) 93 650 16.00 0 1 0.72 0.32
290 473 2bik (B) 139 294 39.00 0.0000000000031 1 0.58 -0.8
90 471 3i6u (A) 152 488 26.00 0 1 0.484106 0.77
100 469 4xbr (A) 176 572 27.00 0 1 0.593786 0.49
103 505 4lqq (D) 313 745 24.00 0 0.98 0.346167 1.85
140 651 3pvu (A) 187 650 22.00 0 1 0.674579 0.95
299 397 4y8d (A) 152 263 44.00 0.000061 0.72 0.213445 1.44
541 653 2p0d (A) 326 435 12.00 0 0.32 -0.0965809 0.17
541 653 1eaz (A) 195 289 26.00 0 1 0.303419 -0.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_154673)

Species Accession Gene Product
Leishmania braziliensis LbrM.32.1990   protein kinase, putative
Leishmania donovani LdBPK_321900.1   protein kinase, putative
Leishmania infantum LinJ.32.1900   protein kinase, putative
Leishmania major LmjF.32.1810   protein kinase, putative
Leishmania mexicana LmxM.31.1810   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.11.16850   serine/threonine-protein kinase, putative
Trypanosoma brucei Tb927.11.15010   Serine/threonine-protein kinase NEK21, putative
Trypanosoma congolense TcIL3000.11.15210   serine/threonine-protein kinase, putative
Trypanosoma cruzi TcCLB.508445.30   Serine/threonine-protein kinase NEK21, putative
Trypanosoma cruzi TcCLB.511715.20   Serine/threonine-protein kinase NEK21, putative

Essentiality

LmjF.32.1810 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.6650 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.6650 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.6650 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.6650 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Cell division protein kinase 5 292 aa 24.7% 352 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0027 1 0.5
0.0012 0.5 0.5
0.0069 0.3067 1
0.0032 0.5 0.5
0.0026 0.5 0.5
0.0091 1 0.5
0.0092 1 0.5
0.0007 0.5 0.5
0.0003 0.5 0.5
0.0061 0.6883 0.5304
0.0063 0.7244 0.2543
0.0039 0.5 0.5
0.0039 0.9485 0.5
0.0033 1 1
0.0022 0.5 0.5
0.0036 0.5 0.5
0.0008 0.5 0.5
0.0081 1 0.5
0.0066 0.3101 0
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0059 1 1
0.0067 0.5 0.5
0.0042 0.5 0.5
0.0039 0.5 0.5
0.0098 0.3242 0.2614
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0063 1 1
0.0056 1 0.5
0.0088 0.4477 1
0.0062 0.6935 0
0.0093 0.8828 0
0.0029 0.5 0.5
0.0018 0.5 0.5
0.0037 1 0.5
0.0023 0.5 0.5
0.0011 1 0.5
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0064 0.3377 0
0.0059 1 1
0.0007 0.5 0.5
0.0033 0.5 0.5
0.0032 0.5 0.5
0.0059 1 1
0.0016 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

22 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.32.1810 (Leishmania major), protein kinase, putative
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