Detailed view for LmjF.05.0970

Basic information

TDR Targets ID: 28133
Leishmania major, 3-mercaptopyruvate sulfurtransferase
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2879 | Length (AA): 370 | MW (Da): 40155 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00581   Rhodanese-like domain
PF09122   Domain of unknown function (DUF1930)

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 370 1okg (A) 7 370 91.00 0 1 1.93 -0.88
7 370 1okg (A) 7 370 99.00 0 1 2.13338 -0.87

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 1OKG:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127129)

Species Accession Gene Product
Arabidopsis thaliana AT1G16460   thiosulfate/3-mercaptopyruvate sulfurtransferase 2
Brugia malayi Bm1_51915   Rhodanese-like domain containing protein
Candida albicans CaO19.1356   similar to rat mercaptopyruvate sulfurtransferase (D50564, EC 2.8.1.2) and to S. cerevisiae YOR251C
Caenorhabditis elegans CELE_R186.6   Protein MPST-7
Caenorhabditis elegans CELE_H12D21.7   Protein MPST-3, isoform B
Caenorhabditis elegans CELE_H12D21.4   Protein MPST-2
Dictyostelium discoideum DDB_G0291816   hypothetical protein
Escherichia coli b1757   molybdopterin synthase sulfurtransferase
Escherichia coli b2521   3-mercaptopyruvate sulfurtransferase
Giardia lamblia GL50803_10783   Thiosulfate sulfurtransferase
Homo sapiens ENSG00000128311   thiosulfate sulfurtransferase (rhodanese)
Homo sapiens ENSG00000128309   mercaptopyruvate sulfurtransferase
Leishmania braziliensis LbrM.05.0970   3-mercaptopyruvate sulfurtransferase
Leishmania donovani LdBPK_050970.1   3-mercaptopyruvate sulfurtransferase
Leishmania infantum LinJ.05.0970   3-mercaptopyruvate sulfurtransferase
Leishmania major LmjF.05.0970   3-mercaptopyruvate sulfurtransferase
Leishmania mexicana LmxM.05.0970   mercaptopyruvate sulfurtransferase
Loa Loa (eye worm) LOAG_07696   hypothetical protein
Loa Loa (eye worm) LOAG_08439   hypothetical protein
Mycobacterium leprae ML0728c   Probable thiosulfate sulfurtransferase SseA (RHODANESE) (THIOSULFATE CYANIDE TRANSSULFURASE) (THIOSULFATE THIOTRANSFERASE)
Mycobacterium leprae ML2198   PROBABLE THIOSULFATE SULFURTRANSFERASE CYSA2 (RHODANESE-LIKE PROTEIN) (THIOSULFATE CYANIDE TRANSSULFURASE) (THIOSULFATE THIOTRAN
Mus musculus ENSMUSG00000044986   thiosulfate sulfurtransferase, mitochondrial
Mus musculus ENSMUSG00000071711   mercaptopyruvate sulfurtransferase
Mycobacterium tuberculosis Rv3283   Probable thiosulfate sulfurtransferase SseA (rhodanese) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase)
Mycobacterium tuberculosis Rv0815c   Probable thiosulfate sulfurtransferase CysA2 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotran
Mycobacterium tuberculosis Rv3117   Probable thiosulfate sulfurtransferase CysA3 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotran
Mycobacterium tuberculosis Rv2291   Probable thiosulfate sulfurtransferase SseB
Mycobacterium ulcerans MUL_2635   thiosulfate sulfurtransferase SseA
Mycobacterium ulcerans MUL_0432   thiosulfate sulfurtransferase, CysA2
Neospora caninum NCLIV_070240   Thiosulfate sulfurtransferase, related
Oryza sativa 4352752   Os12g0608600
Oryza sativa 4328418   Os02g0167100
Plasmodium berghei PBANKA_0605000   rhodanese like protein, putative
Plasmodium falciparum PF3D7_1206400   rhodanese like protein, putative
Plasmodium knowlesi PKNH_1306400   rhodanese like protein, putative
Plasmodium vivax PVX_084355   rhodanese like protein, putative
Plasmodium yoelii PY01845   hypothetical protein
Saccharomyces cerevisiae YOR251C   Tum1p
Trypanosoma brucei gambiense Tbg972.7.6900   mercaptopyruvate sulfurtransferase, putative
Trypanosoma brucei Tb927.7.5920   mercaptopyruvate sulfurtransferase, putative
Trypanosoma congolense TcIL3000_7_4940   mercaptopyruvate sulfurtransferase, putative
Trypanosoma cruzi TcCLB.508173.40   mercaptopyruvate sulfurtransferase, putative
Toxoplasma gondii TGME49_305910   hypothetical protein
Trichomonas vaginalis TVAG_129830   thiosulfate sulfertansferase, putative

Essentiality

LmjF.05.0970 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu3170 Mycobacterium tuberculosis non-essential nmpdr
Tb927.7.5920 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.5920 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.5920 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.5920 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b1757 Escherichia coli non-essential goodall
b2521 Escherichia coli non-essential goodall
TGME49_305910 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0005 0.3625 0.3625

Assayability

Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Leishmania major ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    LmjF.05.0970 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Leishmania major ( 1 )

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.05.0970 (Leishmania major), 3-mercaptopyruvate sulfurtransferase
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