Detailed view for PF3D7_1454500

Basic information

TDR Targets ID: 2848
Plasmodium falciparum, iron sulfur cluster assembly protein, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.2334 | Length (AA): 162 | MW (Da): 18057 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01592   NifU-like N terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005739   mitochondrion  
GO:0051536   iron-sulfur cluster binding  
GO:0005515   protein binding  
GO:0005506   iron ion binding  
GO:0016226   iron-sulfur cluster assembly  
GO:0006879   cellular iron ion homeostasis  

Metabolic Pathways

Nitrogen metabolism (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
38 159 1q48 (A) 2 124 59.00 0 1 1.37 -0.28
48 160 1wfz (A) 8 121 64.00 0 1 1.54 -1.12
39 162 4eb5 (C) 2 123 48.00 0 1 1.39783 -1.2
45 162 1wfz (A) 5 123 61.00 0 1 1.4823 -0.87

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, gametocyte, merozoite, sporozoite, early ring, early trophozoite, late ring, Oocyst, Ring, Sporozoite, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont, late schizont, late trophozoite. Otto TD PlasmoDB
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_127020)

Species Accession Gene Product
Arabidopsis thaliana AT4G22220   iron-sulfur cluster assembly protein 1
Arabidopsis thaliana AT4G04080   Iron-sulfur cluster assembly protein 3
Arabidopsis thaliana AT3G01020   iron-sulfur cluster assembly protein 2
Babesia bovis BBOV_IV004050   iron sulfur cluster assembly protein nifU-like, putative
Brugia malayi Bm1_36610   NifU-like protein
Candida albicans CaO19.13901   Iron-sulfur cluster nifU-like protein similar to S. cerevisiae ISU1 (YPL135W) mitochondrial iron-sulfur cluster assembly scaffol
Candida albicans CaO19.6548   Iron-sulfur cluster nifU-like protein similar to S. cerevisiae ISU1 (YPL135W) mitochondrial iron-sulfur cluster assembly scaffol
Candida albicans CaO19_6548   hypothetical protein
Caenorhabditis elegans CELE_Y45F10D.4   Protein Y45F10D.4
Cryptosporidium hominis Chro.60135   IscU-like protein
Cryptosporidium parvum cgd6_1050   IscU-like NifU protein, iron-sulfur protein
Chlamydia trachomatis CT_720   NifU-like protein
Dictyostelium discoideum DDB_G0283003   hypothetical protein
Drosophila melanogaster Dmel_CG9836   CG9836 gene product from transcript CG9836-RA
Escherichia coli b2529   iron-sulfur cluster assembly scaffold protein
Echinococcus granulosus EgrG_000483700   iron sulfur cluster assembly enzyme ISCU
Entamoeba histolytica EHI_049620   Fe-S cluster assembly protein NifU, putative
Echinococcus multilocularis EmuJ_000483700   iron sulfur cluster assembly enzyme ISCU
Giardia lamblia GL50803_15196   NifU-like protein
Homo sapiens ENSG00000136003   iron-sulfur cluster assembly enzyme
Leishmania braziliensis LbrM.34.3580   iron-sulfur cluster assembly protein, putative
Leishmania donovani LdBPK_353670.1   iron-sulfur cluster assembly protein, putative
Leishmania infantum LinJ.35.3670   iron-sulfur cluster assembly protein, putative
Leishmania major LmjF.35.3620   iron-sulfur cluster assembly protein, putative
Leishmania mexicana LmxM.34.3620   iron-sulfur cluster assembly protein, putative
Loa Loa (eye worm) LOAG_08352   hypothetical protein
Mycobacterium leprae ML0597   Possible nitrogen fixation related protein
Mus musculus ENSMUSG00000025825   IscU iron-sulfur cluster scaffold homolog (E. coli)
Mycobacterium tuberculosis Rv1465   Possible nitrogen fixation related protein
Mycobacterium ulcerans MUL_1866   nitrogen fixation-like protein
Neospora caninum NCLIV_051300   Nifu-pending protein, related
Oryza sativa 4339646   Os05g0568100
Oryza sativa 4327858   Os01g0662600
Plasmodium berghei PBANKA_1318200   iron sulfur cluster assembly protein, putative
Plasmodium falciparum PF3D7_1454500   iron sulfur cluster assembly protein, putative
Plasmodium knowlesi PKNH_1227600   iron sulfur cluster assembly protein, putative
Plasmodium vivax PVX_117755   nifU protein, putative
Plasmodium yoelii PY00856   NifU-like N terminal domain, putative
Saccharomyces cerevisiae YOR226C   putative iron-binding protein ISU2
Saccharomyces cerevisiae YPL135W   iron-binding protein ISU1
Schistosoma japonicum Sjp_0006260   ko:K04488 nitrogen fixation protein NifU and related proteins, putative
Schistosoma mansoni Smp_077090   nitrogen fixation protein nifu
Schmidtea mediterranea mk4.000914.06   Iron-sulfur cluster assembly enzyme ISCU, mitochondrial
Schmidtea mediterranea mk4.000455.02   Putative nitrogen fixation protein nifu
Trypanosoma brucei gambiense Tbg972.9.6980   iron-sulfur cluster assembly protein, putative
Trypanosoma brucei Tb927.9.11720   Iron-sulfur cluster assembly protein
Trypanosoma congolense TcIL3000_9_4750   Iron-sulfur cluster assembly protein
Trypanosoma cruzi TcCLB.510667.70   iron-sulfur cluster assembly protein, putative
Trypanosoma cruzi TcCLB.507009.90   iron-sulfur cluster assembly protein, putative
Toxoplasma gondii TGME49_237560   iron-sulfur cluster protein ISCU
Treponema pallidum TP0615   nitrogen fixation protein (nifU)
Theileria parva TP01_0192   NifU protein, putative
Trichomonas vaginalis TVAG_432650   nitrogen fixation protein nifu, putative
Wolbachia endosymbiont of Brugia malayi Wbm0756   scaffold protein

Essentiality

PF3D7_1454500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.2830 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.2830 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.2830 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.2830 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b2529 Escherichia coli essential goodall
CELE_Y45F10D.4 Caenorhabditis elegans embryonic arrest wormbase
CELE_Y45F10D.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y45F10D.4 Caenorhabditis elegans larval arrest wormbase
PBANKA_1318200 Plasmodium berghei Essential plasmo
TGME49_237560 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_1454500 (Plasmodium falciparum), iron sulfur cluster assembly protein, putative
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