Detailed view for LmjF.30.0550

Basic information

TDR Targets ID: 29198
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.1461 | Length (AA): 609 | MW (Da): 68388 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 9

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04791   LMBR1-like membrane protein

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
195 324 4tql (A) 20 156 15.00 0.068 0.03 0.306765 -0.06

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128525)

Species Accession Gene Product
Arabidopsis thaliana AT5G65290   LMBR1-like membrane protein
Brugia malayi Bm1_42180   LMBR1-like conserved region family protein
Caenorhabditis elegans CELE_C47G2.4   Protein C47G2.4
Cryptosporidium hominis Chro.70354   Dkfzp434h2226-prov protein
Cryptosporidium parvum cgd7_3150   eukaryote specific membrane proteins signal peptide plus 8 transmembrane domain
Dictyostelium discoideum DDB_G0281669   hypothetical protein
Dictyostelium discoideum DDB_G0284019   hypothetical protein
Drosophila melanogaster Dmel_CG8135   CG8135 gene product from transcript CG8135-RA
Echinococcus granulosus EgrG_000482300   LMBR1 domain containing protein 2
Entamoeba histolytica EHI_049750   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000482300   LMBR1 domain containing protein 2
Homo sapiens 92255   LMBR1 domain containing 2
Leishmania braziliensis LbrM.30.0630   hypothetical protein, conserved
Leishmania donovani LdBPK_300570.1   LMBR1-like membrane protein, putative
Leishmania infantum LinJ.30.0570   hypothetical protein, conserved
Leishmania major LmjF.30.0550   hypothetical protein, conserved
Leishmania mexicana LmxM.29.0550   hypothetical protein, conserved
Mus musculus ensembl-mmu:ENSMUSG00000039704   LMBR1 domain containing 2
Oryza sativa 4340506   Os06g0218900
Oryza sativa 4330787   Os02g0758100
Onchocerca volvulus OVOC10821  
Schmidtea mediterranea mk4.001622.08  
Trypanosoma brucei gambiense Tbg972.6.1700   hypothetical protein, conserved
Trypanosoma brucei Tb927.6.1980   LMBR1-like membrane protein, putative
Trypanosoma congolense TcIL3000_0_33880   LMBR1-like membrane protein, putative
Trypanosoma congolense TcIL3000_6_1560   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.504071.70   LMBR1-like membrane protein, putative
Trichomonas vaginalis TVAG_333140   conserved hypothetical protein
Trichomonas vaginalis TVAG_208790   conserved hypothetical protein
Trichomonas vaginalis TVAG_351520   conserved hypothetical protein

Essentiality

LmjF.30.0550 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.1980 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.1980 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.1980 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.1980 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.30.0550 (Leishmania major), hypothetical protein, conserved
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