Detailed view for LmjF.34.2110

Basic information

TDR Targets ID: 29744
Leishmania major, cardiolipin synthetase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.1436 | Length (AA): 752 | MW (Da): 84841 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13091   PLD-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
547 705 1byr (A) 2 152 23.00 0 0.96 0.52 -1.23
529 701 4ggj (A) 36 206 20.00 0 1 0.480353 -0.69
547 705 1byr (A) 2 152 23.00 0 1 0.541736 -1
585 667 1byr (A) 35 112 32.00 0.012 0.89 0.418072 0.34

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_129472)

Species Accession Gene Product
Babesia bovis BBOV_II002000   phospholipase D active site motif family protein
Cryptosporidium hominis Chro.30333   cardiolipin synthetase
Cryptosporidium parvum cgd3_2940   probable phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthase, 2x SMART_PLDc domains, possible bacterial origin
Chlamydia trachomatis CT_284   phospholipase D superfamily protein
Dictyostelium discoideum DDB_G0284687   phospholipase D
Escherichia coli b0789   cardiolipin synthase 2
Escherichia coli b1249   cardiolipin synthase 1
Leishmania braziliensis LbrM.20.1600   cardiolipin synthetase, putative
Leishmania donovani LdBPK_341860.1   cardiolipin synthetase, putative
Leishmania infantum LinJ.34.1860   cardiolipin synthetase, putative
Leishmania major LmjF.34.2110   cardiolipin synthetase, putative
Leishmania mexicana LmxM.33.2110   cardiolipin synthetase, putative
Neospora caninum NCLIV_068780   hypothetical protein
Neospora caninum NCLIV_054060   hypothetical protein
Plasmodium berghei PBANKA_0108000   mitochondrial cardiolipin synthase, putative
Plasmodium falciparum PF3D7_0609400   mitochondrial cardiolipin synthase, putative
Plasmodium knowlesi PKNH_1140800   mitochondrial cardiolipin synthase, putative
Plasmodium vivax PVX_113625   cardiolipin synthetase, putative
Plasmodium yoelii PY06901   cardiolipin synthetase
Trypanosoma brucei gambiense Tbg972.4.2520   cardiolipin synthetase, putative
Trypanosoma brucei Tb927.4.2560   cardiolipin synthetase
Trypanosoma brucei Tb11.v5.0672   cardiolipin synthetase, putative
Trypanosoma congolense TcIL3000_4_2440   cardiolipin synthetase, putative
Trypanosoma cruzi TcCLB.506631.20   cardiolipin synthetase, putative
Trypanosoma cruzi TcCLB.506559.40   cardiolipin synthetase, putative
Toxoplasma gondii TGME49_309940   phospholipase D active site domain-containing protein
Theileria parva TP04_0329   cardiolipin synthetase, putative

Essentiality

LmjF.34.2110 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.2560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.2560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.2560 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.2560 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0789 Escherichia coli non-essential goodall
b1249 Escherichia coli non-essential goodall
TGME49_309940 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.34.2110 (Leishmania major), cardiolipin synthetase, putative
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