Detailed view for TcCLB.511727.100

Basic information

TDR Targets ID: 30075
Trypanosoma cruzi, Alpha/beta hydrolase family, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.1378 | Length (AA): 331 | MW (Da): 36375 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12697   Alpha/beta hydrolase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0051723   protein methylesterase activity  
GO:0006482   protein amino acid demethylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 314 1mna (A) 29 288 16.00 0 1 0.79 -0.05
9 315 1u2e (A) 0 283 14.00 0 1 1.1 -0.57
18 315 1iup (A) 3 272 15.00 0 1 1.01 -0.8
3 328 2y6v (A) 10 357 15.00 0.000021 1 1.09689 0.26
4 321 3c5v (A) 41 375 33.00 0 1 1.32473 -0.36
24 100 1imj (A) 11 75 22.00 0.19 0.07 0.0686284 0.89
34 162 3n2z (B) 75 199 16.00 0 0.86 0.482628 -0.5
81 169 4uhc (A) 47 125 34.00 0.25 0.8 0.519782 0.44

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128677)

Species Accession Gene Product
Arabidopsis thaliana AT4G10050   esterase/lipase/thioesterase family protein
Brugia malayi Bm1_23540   protein phosphatase methylesterase 1
Candida albicans CaO19.9034   similar to S. cerevisiae PPE1 (YHR075C) mitochondrial ribosome small subunit protein YMS2, which resembles a phosphatase-methyle
Candida albicans CaO19.1459   similar to S. cerevisiae PPE1 (YHR075C) mitochondrial ribosome small subunit protein YMS2, which resembles a phosphatase-methyle
Caenorhabditis elegans CELE_B0464.9   Protein B0464.9
Dictyostelium discoideum DDB_G0281651   protein phosphatase methylesterase 1
Drosophila melanogaster Dmel_CG5068   CG5068 gene product from transcript CG5068-RB
Echinococcus granulosus EgrG_000879300   protein phosphatase methylesterase 1
Echinococcus granulosus EgrG_000879100   protein phosphatase methylesterase 1
Echinococcus multilocularis EmuJ_000879100   protein phosphatase methylesterase 1
Echinococcus multilocularis EmuJ_000879300   protein phosphatase methylesterase 1
Giardia lamblia GL50803_16180   Protein phosphatase methylesterase-1
Homo sapiens ENSG00000214517   protein phosphatase methylesterase 1
Leishmania braziliensis LbrM.32.1000   hypothetical protein, conserved
Leishmania donovani LdBPK_320960.1   Alpha/beta hydrolase family, putative
Leishmania infantum LinJ.32.0960   hypothetical protein, conserved
Leishmania major LmjF.32.0910   hypothetical protein, conserved
Leishmania mexicana LmxM.31.0910   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_06335   protein phosphatase methylesterase 1
Mus musculus ENSMUSG00000030718   protein phosphatase methylesterase 1
Neospora caninum NCLIV_025490   hypothetical protein
Oryza sativa 4335946   Os04g0443500
Onchocerca volvulus OVOC7916   Putative protein phosphatase methylesterase 1
Saccharomyces cerevisiae YHR075C   carboxylesterase-mitochondrial 37S ribosomal protein YmS2
Schistosoma japonicum Sjp_0062170   ko:K01066 esterase / lipase [EC3.1.1.-], putative
Schistosoma mansoni Smp_149920   protein phosphatase methylesterase 1 (S33 family)
Schmidtea mediterranea mk4.024922.00   Protein phosphatase methylesterase 1
Schmidtea mediterranea mk4.009781.00   Protein phosphatase methylesterase 1
Schmidtea mediterranea mk4.017801.00   Protein phosphatase methylesterase 1
Trypanosoma brucei gambiense Tbg972.11.15820   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.14160   Alpha/beta hydrolase family, putative
Trypanosoma congolense TcIL3000.11.14460   Alpha/beta hydrolase family, putative
Trypanosoma cruzi TcCLB.511727.100   Alpha/beta hydrolase family, putative
Toxoplasma gondii TGME49_262140   chain a, pp2a-specific methylesterase apo form (pme), putative
Trichomonas vaginalis TVAG_466760   Clan SC, family S33, methylesterase-like serine peptidase

Essentiality

TcCLB.511727.100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.5750 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.5750 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.5750 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.5750 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_B0464.9 Caenorhabditis elegans embryonic lethal wormbase
CELE_B0464.9 Caenorhabditis elegans larval arrest wormbase
TGME49_262140 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mus musculus protein phosphatase methylesterase 1 Compounds References
Homo sapiens protein phosphatase methylesterase 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0684 1 1
0.0906 1 1
0.06 1 1
0.0684 1 1
0.0925 1 1
0.0602 0.6097 1
0.0161 0.2817 0.7339
0.0933 0.9519 1
0.0933 0.9519 1
0.0461 1 1
0.0161 1 1
0.0438 0.2704 1
0.0789 1 1
0.0161 1 0.5
0.0161 0.4165 1
0.0276 0.3184 1
0.1235 0.5092 1
0.1097 1 1
0.008 0.4911 1
0.073 1 1
0.0925 1 1
0.1025 0.762 1
0.1025 0.762 1
0.1048 1 1
0.0161 1 0.5
0.0161 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.511727.100 (Trypanosoma cruzi), Alpha/beta hydrolase family, putative
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