Detailed view for TcCLB.503999.50

Basic information

TDR Targets ID: 30086
Trypanosoma cruzi, ubiquitin-conjugating enzyme, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.0957 | Length (AA): 138 | MW (Da): 15765 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00179   Ubiquitin-conjugating enzyme

Gene Ontology

Mouse over links to read term descriptions.
GO:0019787   small conjugating protein ligase activity  
GO:0051246   regulation of protein metabolic process  
GO:0043687   post-translational protein modification  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 138 2a4d (A) 83 219 47.00 0 1 1.74385 -1.98
2 138 1jat (B) 2 137 55.00 0.000000000013 1 1.75405 -1.5
4 138 2q0v (A) 20 153 54.00 0 1 1.76936 -1.68
39 133 2gjd (A) 38 131 23.00 0.00046 0.99 1.10251 -1.02

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127771)

Species Accession Gene Product
Arabidopsis thaliana AT1G70660   ubiquitin-conjugating enzyme E2 variant 1B
Arabidopsis thaliana AT3G52560   ubiquitin-conjugating enzyme E2 variant 1D
Arabidopsis thaliana AT2G36060   MMS ZWEI-like protein 3
Arabidopsis thaliana AT1G23260   MMS ZWEI-like protein 1
Babesia bovis BBOV_III004030   conserved hypothetical protein
Candida albicans CaO19.13715   ubiquitin conjugating enzyme
Candida albicans CaO19.6358   ubiquitin conjugating enzyme
Caenorhabditis elegans CELE_F39B2.2   Protein UEV-1
Cryptosporidium parvum cgd8_580   ubiquitin-conjugating enzyme E2, putative
Dictyostelium discoideum DDB_G0292596   hypothetical protein
Drosophila melanogaster Dmel_CG10640   CG10640 gene product from transcript CG10640-RB
Homo sapiens ENSG00000124208   TMEM189-UBE2V1 readthrough
Homo sapiens 102723952   ubiquitin-conjugating enzyme E2 variant 1-like
Homo sapiens 102724605   ubiquitin-conjugating enzyme E2 variant 1-like
Homo sapiens 7336   ubiquitin-conjugating enzyme E2 variant 2
Leishmania braziliensis LbrM.13.1440   ubiquitin-conjugating enzyme-like protein
Leishmania donovani LdBPK_131320.1   ubiquitin-conjugating enzyme, putative
Leishmania infantum LinJ.13.1320   ubiquitin-conjugating enzyme-like protein
Leishmania major LmjF.13.1580   ubiquitin-conjugating enzyme-like protein
Leishmania mexicana LmxM.13.1580   ubiquitin-conjugating enzyme-like protein
Loa Loa (eye worm) LOAG_04871   hypothetical protein
Mus musculus ENSMUSG00000078923   ubiquitin-conjugating enzyme E2 variant 1
Neospora caninum NCLIV_054150   Ubiquitin-conjugating enzyme, related
Oryza sativa 4352739   Os12g0605400
Oryza sativa 4346652   Os09g0297100
Oryza sativa 4333892   Os03g0712300
Plasmodium berghei PBANKA_0404200   ubiquitin-conjugating enzyme E2, putative
Plasmodium falciparum PF3D7_0305700   ubiquitin-conjugating enzyme E2, putative
Plasmodium knowlesi PKNH_0837000   ubiquitin-conjugating enzyme E2, putative
Plasmodium vivax PVX_119410   ubiquitin-conjugating enzyme E2, putative
Plasmodium yoelii PY05886   Plasmodium vivax PV1H14205_P
Saccharomyces cerevisiae YGL087C   E2 ubiquitin-conjugating protein MMS2
Schistosoma japonicum Sjp_0306700   Ubiquitin-conjugating enzyme E2 variant 2, putative
Schmidtea mediterranea mk4.002991.02  
Trypanosoma brucei gambiense Tbg972.11.3720   ubiquitin-conjugating enzyme, putative,ubiquitin-conjugating enzyme-like protein, putative
Trypanosoma brucei Tb927.11.3310   ubiquitin-conjugating enzyme, putative
Trypanosoma congolense TcIL3000.11.3140   ubiquitin-conjugating enzyme, putative
Trypanosoma cruzi TcCLB.503999.50   ubiquitin-conjugating enzyme, putative
Trypanosoma cruzi TcCLB.509591.10   ubiquitin-conjugating enzyme, putative
Toxoplasma gondii TGME49_310040   ubiquitin conjugating enzyme E2, putative
Theileria parva TP03_0025   Ubiquitin-conjugating enzyme, putative
Trichomonas vaginalis TVAG_254880   ubiquitin-conjugating enzyme, putative
Trichomonas vaginalis TVAG_225740   ubiquitin-conjugating enzyme E2, putative

Essentiality

TcCLB.503999.50 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.0815 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.0815 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.0815 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.02.0815 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0404200 Plasmodium berghei Essential plasmo
TGME49_310040 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TcCLB.503999.50 (Trypanosoma cruzi), ubiquitin-conjugating enzyme, putative
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