Detailed view for TcCLB.510733.10

Basic information

TDR Targets ID: 30662
Trypanosoma cruzi, p21-activated kinase 3, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.2935 | Length (AA): 549 | MW (Da): 61864 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
112 536 1fmk () 106 525 20.00 0 1 0.97 0.3
252 549 1yhv (A) 249 539 37.00 0.00000002 1 1.07 -1.08
35 145 1upq (A) 59 152 24.00 0.00075 0.44 0.176386 0.85
152 524 4fl2 (A) 180 622 22.00 0 1 0.868617 0.26
252 544 4ks7 (A) 386 671 38.00 0 1 1.0269 -0.78
262 549 1u5q (A) 17 298 38.00 0 1 0.98079 -0.81
266 549 4lg4 (A) 20 296 38.00 0 1 1.0095 -0.87
268 530 2x7f (E) 20 289 44.00 0 1 0.841453 -0.19

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127124)

Species Accession Gene Product
Brugia malayi Bm1_31335   p21/Cdc42/Rac1-activated kinase
Brugia malayi Bm1_34325   Protein kinase domain
Candida albicans CaO19.11717   likely signal transduction kinase
Candida albicans CaO19.12355   serine-threonine kinase
Candida albicans CaO19.4890   serine-threonine kinase
Candida albicans CaO19.4242   likely signal transduction kinase
Caenorhabditis elegans CELE_Y38F1A.10   Protein MAX-2, isoform D
Caenorhabditis elegans CELE_C09B8.7   Protein PAK-1, isoform E
Dictyostelium discoideum DDB_G0269696   PAKA subfamily protein kinase
Dictyostelium discoideum DDB_G0267450   PAKA subfamily protein kinase
Dictyostelium discoideum DDB_G0269166   PAKA subfamily protein kinase
Dictyostelium discoideum DDB_G0276459   PAKA subfamily protein kinase
Drosophila melanogaster Dmel_CG10295   PAK-kinase
Drosophila melanogaster Dmel_CG14895   CG14895 gene product from transcript CG14895-RD
Echinococcus granulosus EgrG_000423000   serine:threonine protein kinase PAK 3
Echinococcus granulosus EgrG_001181300   serine:threonine protein kinase PAK 3
Echinococcus granulosus EgrG_001167000   serine:threonine protein kinase PAK 1
Echinococcus granulosus EgrG_001167100   p21 activated protein kinase 1 Dpak1
Entamoeba histolytica EHI_186750   protein kinase, putative
Entamoeba histolytica EHI_148280   p21-activated kinase
Echinococcus multilocularis EmuJ_000423000   serine:threonine protein kinase PAK 3
Echinococcus multilocularis EmuJ_001167100   p21 activated protein kinase 1 Dpak1
Echinococcus multilocularis EmuJ_001181300   serine:threonine protein kinase PAK 3
Giardia lamblia GL50803_2796   Kinase, STE STE20
Homo sapiens ENSG00000077264   p21 protein (Cdc42/Rac)-activated kinase 3
Homo sapiens ENSG00000149269   p21 protein (Cdc42/Rac)-activated kinase 1
Homo sapiens ENSG00000180370   p21 protein (Cdc42/Rac)-activated kinase 2
Loa Loa (eye worm) LOAG_07996   STE/STE20/PAKA protein kinase
Loa Loa (eye worm) LOAG_08976   STE/STE20/PAKA protein kinase
Loa Loa (eye worm) LOAG_10648   hypothetical protein
Mus musculus ENSMUSG00000030774   p21 protein (Cdc42/Rac)-activated kinase 1
Mus musculus ENSMUSG00000022781   p21 protein (Cdc42/Rac)-activated kinase 2
Mus musculus ensembl-mmu:ENSMUSG00000031284   p21 protein (Cdc42/Rac)-activated kinase 3
Saccharomyces cerevisiae YHL007C   Ste20p
Saccharomyces cerevisiae YNL298W   Cla4p
Saccharomyces cerevisiae YOL113W   Skm1p
Schistosoma japonicum Sjp_0109470   Serine/threonine-protein kinase PAK 3, putative
Schistosoma japonicum Sjp_0131810   IPR000095,PAK-box/P21-Rho-binding;IPR011026,Wiscott-Aldrich syndrome, C-terminal,domain-containing
Schistosoma japonicum Sjp_0111780   Serine/threonine-protein kinase PAK 3, putative
Schistosoma japonicum Sjp_0002460   ko:K04409 p21-activated kinase 1, putative
Schistosoma japonicum Sjp_0013390   IPR000719,Protein kinase;IPR011009,Protein kinase-like,domain-containing
Schistosoma japonicum Sjp_0013400   Serine/threonine-protein kinase PAK 1, putative
Schistosoma japonicum Sjp_0317790   Serine/threonine-protein kinase STE20, putative
Schistosoma japonicum Sjp_0074700   Serine/threonine-protein kinase pak-1, putative
Schistosoma mansoni Smp_064040.1   protein kinase
Schistosoma mansoni Smp_179800   serine/threonine protein kinase
Schistosoma mansoni Smp_175160   serine/threonine protein kinase
Schistosoma mansoni Smp_064040.2   protein kinase
Schmidtea mediterranea mk4.009413.00  
Schmidtea mediterranea mk4.037521.00   P21 activated kinase-1
Schmidtea mediterranea mk4.000464.00  
Schmidtea mediterranea mk4.015333.00  
Schmidtea mediterranea mk4.011904.00  
Schmidtea mediterranea mk4.005838.00  
Schmidtea mediterranea mk4.010163.01  
Schmidtea mediterranea mk4.009413.01  
Schmidtea mediterranea mk4.016010.04  
Schmidtea mediterranea mk4.021674.00  
Schmidtea mediterranea mk4.001197.01  
Schmidtea mediterranea mk4.000464.02   Cell polarity protein
Schmidtea mediterranea mk4.002396.02  
Trypanosoma cruzi TcCLB.509551.10   STE/STE20 serine/threonine-protein kinase, putative
Trypanosoma cruzi TcCLB.510733.10   p21-activated kinase 3, putative
Trichomonas vaginalis TVAG_116130   STE family protein kinase
Trichomonas vaginalis TVAG_094100   STE family protein kinase
Trichomonas vaginalis TVAG_434790   STE family protein kinase
Trichomonas vaginalis TVAG_492470   conserved hypothetical protein
Trichomonas vaginalis TVAG_235640   mitogen-activated protein kinase kinase kinase 3, MAPKKK3, MEKK3, putative
Trichomonas vaginalis TVAG_442880   STE family protein kinase

Essentiality

TcCLB.510733.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_C09B8.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_C09B8.7 Caenorhabditis elegans larval lethal wormbase
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens p21 protein (Cdc42/Rac)-activated kinase 1 Compounds References
Homo sapiens p21 protein (Cdc42/Rac)-activated kinase 3 Compounds References
Homo sapiens p21 protein (Cdc42/Rac)-activated kinase 2 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 29.4% 289 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 35.3% 173 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 27.5% 171 aa Compounds References
Patiria pectinifera Cdc2 300 aa 29.3% 287 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 23.3% 309 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 25.9% 263 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 24.4% 303 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 29.6% 287 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 30.1% 282 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0533 0.5774 1
0.0039 0.5 0.5
0.0018 0.5 0.5
0.0003 0.5 0.5
0.0053 0.2768 0.5
0.0332 0.2768 0.5
0.0053 0.2768 0.5
0.016 0.4466 0.5
0.053 0.3602 1
0.0088 0.3791 1
0.0162 0.5471 0.5
0.0629 0.4076 1
0.0026 0.2768 0.5
0.0234 0.3516 1
0.0079 0.2768 0.5
0.0059 1 1
0.0012 0.5 0.5
0.0053 0.2768 0.5
0.0079 0.2768 0.5
0.0079 0.2768 0.5
0.0026 0.2768 0.5
0.043 0.5759 1
0.0678 0.4158 1
0.0189 0.5509 0.5
0.033 0.2768 0.5
0.0136 0.2633 1
0.0067 0.2768 0.5
0.0004 0.5 0.5
0.0012 0.5 0.5
0.0069 0.3067 1
0.053 0.3602 1
0.0075 0.2768 0.5
0.0023 0.5 0.5
0.0526 0.5562 1
0.0037 1 0.5
0.0678 0.4158 1
0.053 0.3602 1
0.0059 1 1
0.0053 0.2768 0.5
0.0026 0.5 0.5
0.0063 1 0.5
0.0145 0.4484 0.5
0.0655 0.5683 1
0.0348 0.2768 0.5
0.0141 0.3198 0.5
0.0026 0.2768 0.5
0.0053 0.2768 0.5
0.0191 0.5532 0.5
0.0039 0.5 0.5
0.0042 0.5 0.5
0.0079 0.2768 0.5
0.0091 1 0.5
0.0139 0.4583 1
0.0073 0.2768 0.5
0.0075 0.254 0.5
0.0142 0.5375 0.5
0.0303 0.5416 0.5
0.0044 0.2768 0.5
0.0032 0.5 0.5
0.0033 0.5 0.5
0.0079 0.2768 0.5
0.051 0.4526 0.5
0.0234 0.3516 1
0.0068 0.4734 1
0.0517 0.255 0.5
0.0189 0.5541 0.5
0.0233 0.4908 0.5
0.0022 0.5 0.5
0.0296 0.5691 0.5
0.0364 0.2768 0.5
0.0079 0.2768 0.5
0.0329 0.2768 0.5
0.0079 0.2768 0.5
0.0008 0.5 0.5
0.0053 0.2768 0.5
0.0062 0.6935 0
0.0132 0.462 0.5
0.0166 0.33 0
0.053 0.3602 1
0.053 0.4842 0.5
0.0291 0.5777 1
0.017 0.5499 1
0.0027 1 0.5
0.0053 0.2768 0.5
0.0576 0.5761 1
0.0007 0.5 0.5
0.0221 0.4976 0.5
0.0053 0.2768 0.5
0.0381 0.2768 0.5
0.0512 0.3252 1
0.0079 0.2768 0.5
0.0164 0.5598 0.5
0.0016 0.5 0.5
0.0007 0.5 0.5
0.0526 0.5562 1
0.0011 1 0.5
0.0139 0.2573 0.5
0.0063 0.7244 0.2543
0.0171 0.5408 0.5
0.0479 0.2627 1
0.0629 0.4076 1
0.058 0.5744 1
0.0092 1 0.5
0.0029 0.5 0.5
0.0067 0.2768 0.5
0.053 0.3602 1
0.0056 1 0.5
0.0525 0.2698 0.5
0.0053 0.2768 0.5
0.0065 0.2768 0.5
0.0629 0.4076 1
0.0487 0.4947 1
0.018 0.2768 0.5
0.0066 0.3101 0
0.0012 0.5 0.5
0.0531 0.4004 0.5
0.0677 0.3181 0.5
0.0137 0.2768 0.5
0.0479 0.2627 1
0.0079 0.2768 0.5
0.0053 0.2768 0.5
0.0329 0.2768 0.5
0.0156 0.4537 0.5
0.0079 0.2768 0.5
0.0032 0.5 0.5
0.0033 1 0.5
0.0081 0.5 0.5
0.0007 0.5 0.5
0.0162 0.5471 0.5
0.053 0.3602 1
0.0077 0.2768 0.5
0.0063 0.564 0.5
0.0059 1 1
0.0098 0.3242 0.3649
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0479 0.3957 0.5
0.0677 0.3181 0.5
0.0064 0.3377 0
0.0211 0.2768 0.5
0.023 0.547 0.5
0.0158 0.5632 0.5
0.0093 0.8828 0
0.0088 0.2768 0.5
0.017 0.5688 0.5
0.017 0.5499 1
0.0208 0.5436 0.5
0.0331 0.2768 0.5
0.0678 0.4158 1
0.015 0.287 0.5
0.0079 0.2768 0.5
0.0678 0.4158 1
0.0088 0.4477 0.5
0.0455 0.2768 0.5
0.0153 0.2768 0.5
0.0122 0.2923 1
0.0629 0.4076 1
0.0401 0.3501 0.5
0.0172 0.5401 0.5
0.0039 0.9485 0.5
0.0079 0.2768 0.5
0.0081 1 0.5
0.0079 0.2768 0.5
0.0162 0.5471 0.5
0.0077 0.2768 0.5
0.022 0.2768 0.5
0.0479 0.3957 0.5
0.0036 0.5 0.5
0.0576 0.5761 1
0.0061 0.6883 0.5304
0.0598 0.5595 0.5
0.0512 0.3252 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.510733.10 (Trypanosoma cruzi), p21-activated kinase 3, putative
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