Detailed view for TcCLB.506797.100

Basic information

TDR Targets ID: 30957
Trypanosoma cruzi, beta-ketoacyl-ACP reductase 2, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 10.1018 | Length (AA): 594 | MW (Da): 66767 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 4

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00106   short chain dehydrogenase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
261 526 1pr9 (A) 5 243 21.00 0 1 0.6 -0.69
264 569 1n5d (A) 5 282 22.00 0 0.99 0.46 0.08
265 563 1wma (A) 6 276 20.00 0.0000000026 0.96 0.67 -0.33
266 399 1yde (A) 12 140 29.00 0.000067 0.99 0.6 -1.22
220 428 3u0b (A) 156 377 35.00 0.0000000023 1 0.551352 0.69
246 286 2bel (C) 18 57 45.00 0 0.28 0.485424 -0.12
260 519 5t2v (A) 2 232 28.00 0 1 0.58611 -0.25
264 305 5f5n (A) 6 46 41.00 0 0.87 0.592107 -0.97
267 352 2qq5 (A) 11 93 34.00 0.19 0.91 0.529281 -0.25

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_135949)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG31235   CG31235 gene product from transcript CG31235-RB
Leishmania braziliensis LbrM.24.1880   short chain dehydrogenase/reductase, putative
Leishmania donovani LdBPK_241880.1   short chain dehydrogenase/reductase, putative
Leishmania infantum LinJ.24.1880   short chain dehydrogenase/reductase, putative
Leishmania major LmjF.24.1810   short chain dehydrogenase/reductase, putative
Leishmania mexicana LmxM.24.1810   short chain dehydrogenase/reductase, putative
Neospora caninum NCLIV_053680   hypothetical protein
Trypanosoma brucei gambiense Tbg972.8.6480   short-chain dehydrogenase, putative
Trypanosoma brucei Tb927.8.6420   beta-ketoacyl-ACP reductase 2
Trypanosoma cruzi TcCLB.511907.80   beta-ketoacyl-ACP reductase 2, putative
Trypanosoma cruzi TcCLB.506797.100   beta-ketoacyl-ACP reductase 2, putative
Toxoplasma gondii TGME49_309265   oxidoreductase, short chain dehydrogenase/reductase family protein
Trichomonas vaginalis TVAG_354120   short-chain dehydrogenase, putative

Essentiality

TcCLB.506797.100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.6420 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.6420 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.6420 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.8.6420 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_309265 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Macaca mulatta Hydroxysteroid 11-beta dehydrogenase 1 140 aa 29.2% 144 aa Compounds References

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0058 1 1
0.0004 0.5 0.5
0.0108 0.5 0.5
0.0019 0.4322 1
0.0012 0.5 0.5
0.0106 1 1
0.0196 0.8679 0.5
0.0053 1 1
0.0088 0.6544 1
0.0032 1 1
0.0087 0.5 0.5
0.0089 0.5 0.5
0.016 0.577 1
0.0066 0.3586 0.5
0.004 0.5 0.5
0.0114 1 0.5
0.0016 0.5 0.5
0.0045 1 1
0.0027 0.5 0.5
0.0009 1 0.5
0.0165 1 1
0.0157 0.5 0.5
0.0004 0.5 0.5
0.0119 0.2746 1
0.0027 0.5 0.5
0.0089 0.5 0.5
0.007 0.2916 1
0.0004 0.5 0.5
0.0009 0.5 0.5
0.0005 0.5 0.5
0.0082 1 0.5
0.0071 0.3141 0.5
0.0408 0.5144 1
0.0073 0.4546 1
0.0068 0.2585 0.5
0.0009 0.7374 0.5
0.0106 1 0.5
0.0093 0.5 0.5
0.0106 0.2872 1
0.0132 0.3658 1
0.0131 0.5 0.5
0.0196 0.8679 0.5
0.0102 0.7451 0.5
0.008 0.5 0.5
0.0068 1 1
0.0009 0.5 0.5
0.0058 0.3931 1
0.0196 0.8679 0.5
0.0039 1 1
0.0097 1 1
0.0021 0.3321 0.5
0.0085 1 1
0.0408 0.5144 1
0.0218 0.5 0.5
0.0064 0.3508 0.5
0.0026 0.5 0.5
0.0123 0.5 0.5
0.0004 0.5 0.5
0.0021 0.5 0.5
0.0113 1 1
0.0171 0.4063 0.5
0.007 1 1
0.0009 0.5 0.5
0.0004 0.5 0.5
0.0073 0.3811 1
0.007 0.2916 1
0.0053 1 1
0.0106 0.5 0.5
0.0004 0.5 0.5
0.0088 1 1
0.0114 1 0.5
0.0162 1 1
0.0021 0.3321 0.5
0.0097 0.4304 1
0.0222 0.5 0.5
0.0067 0.3468 1
0.0013 1 0.5
0.0056 1 1
0.0084 0.4283 0.4047
0.0004 0.5 0.5
0.0056 0.875 1
0.0073 0.3141 1
0.0171 0.4063 0.5
0.0009 0.5 0.5
0.007 0.2916 1
0.0012 0.5 0.5
0.0073 0.3811 1
0.007 1 1
0.011 0.3568 1
0.0402 1 1
0.0009 0.5 0.5
0.007 1 1
0.0073 0.2873 1
0.0057 0.284 0.6352
0.0118 1 1
0.0408 0.5144 1
0.0046 0.3082 0
0.0009 0.5 0.5
0.0005 0.5 0.5
0.0035 1 1
0.0009 0.5 0.5
0.0013 0.5 0.5
0.0009 0.5 0.5
0.0068 1 1
0.016 0.577 1
0.0076 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.506797.100 (Trypanosoma cruzi), beta-ketoacyl-ACP reductase 2, putative
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