Detailed view for TcCLB.511303.160

Basic information

TDR Targets ID: 32080
Trypanosoma cruzi, sodium/hydrogen exchanger, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.197 | Length (AA): 676 | MW (Da): 74452 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 11

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00999   Sodium/hydrogen exchanger family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0015299   solute:hydrogen antiporter activity  
GO:0055085   transmembrane transport  
GO:0006812   cation transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
128 497 1u7g (A) 20 385 13.00 0.0000023 0.15 0.68 -2.13
179 644 1xme (A) 35 517 14.00 0.000053 0.05 0.74 -0.61
21 134 5j0l (A) 14 131 9.00 0.072 0.02 0.317639 -0.99
149 524 3nmo (A) 48 439 17.00 0 0.7 0.585813 0.66
186 564 5bz3 (A) 11 345 21.00 0 0.65 0.602251 0.79

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_128860)

Species Accession Gene Product
Caenorhabditis elegans CELE_F41E7.1   Protein F41E7.1
Caenorhabditis elegans CELE_F57G8.5   Protein F57G8.5
Caenorhabditis elegans CELE_F41E7.2   Protein F41E7.2
Drosophila melanogaster Dmel_CG10806   Na[+]/H[+] hydrogen antiporter 1
Drosophila melanogaster Dmel_CG43442   Na[+]/H[+] hydrogen antiporter 2
Echinococcus granulosus EgrG_000546600   mitochondrial sodium:hydrogen exchanger
Echinococcus granulosus EgrG_000546700   mitochondrial sodium:hydrogen exchanger 9B2
Echinococcus granulosus EgrG_000546500   mitochondrial sodium:hydrogen exchanger
Echinococcus multilocularis EmuJ_000546600   mitochondrial sodium:hydrogen exchanger
Echinococcus multilocularis EmuJ_000546700   mitochondrial sodium:hydrogen exchanger 9B2
Echinococcus multilocularis EmuJ_000546500   mitochondrial sodium:hydrogen exchanger
Homo sapiens ENSG00000164038   solute carrier family 9, subfamily B (NHA2, cation proton antiporter 2), member 2
Homo sapiens 102723912   sodium/hydrogen exchanger 9B1-like
Homo sapiens ENSG00000164037   solute carrier family 9, subfamily B (NHA1, cation proton antiporter 1), member 1
Homo sapiens 101929373   sodium/hydrogen exchanger 9B1-like
Leishmania braziliensis LbrM.14.0960   hypothetical protein, conserved
Leishmania donovani LdBPK_141040.1   sodium/hydrogen exchanger, putative
Leishmania infantum LinJ.14.1040   hypothetical protein, conserved
Leishmania major LmjF.14.0980   hypothetical protein, conserved
Leishmania mexicana LmxM.14.0980   hypothetical protein, conserved
Mus musculus ENSMUSG00000050150   solute carrier family 9, subfamily B (NHA1, cation proton antiporter 1), member 1
Mus musculus ENSMUSG00000037994   solute carrier family 9, subfamily B (NHA2, cation proton antiporter 2), member 2
Onchocerca volvulus OVOC4415  
Schistosoma japonicum Sjp_0095900   Sodium/hydrogen exchanger-like domain-containing protein 1, putative
Schistosoma mansoni Smp_175980   hypothetical protein
Trypanosoma brucei gambiense Tbg972.11.880   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.840   sodium/hydrogen exchanger, putative
Trypanosoma congolense TcIL3000_5_2370   sodium/hydrogen exchanger, putative
Trypanosoma congolense TcIL3000_0_07180   sodium/hydrogen exchanger, putative
Trypanosoma cruzi TcCLB.511303.160   sodium/hydrogen exchanger, putative
Trypanosoma cruzi TcCLB.511761.50   sodium/hydrogen exchanger, putative

Essentiality

TcCLB.511303.160 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.03.0300 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.03.0300 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.03.0300 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.03.0300 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.511303.160 (Trypanosoma cruzi), sodium/hydrogen exchanger, putative
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