Detailed view for TGME49_223840

Basic information

TDR Targets ID: 324396
Toxoplasma gondii, ATP-citrate lyase, putative
Source Database / ID:  ToxoDB 

pI: 6.5837 | Length (AA): 1281 | MW (Da): 139836 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00285   Citrate synthase, C-terminal domain
PF00549   CoA-ligase
PF02629   CoA binding domain
PF16114   ATP citrate lyase citrate-binding

Gene Ontology

Mouse over links to read term descriptions.
GO:0046912   transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer  
GO:0048037   cofactor binding  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0044262   cellular carbohydrate metabolic process  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 987 3pff (A) 3 812 51.00 0 1 0.948011 0.59
2 505 3mwd (A) 3 419 48.00 0 1 0.756443 0
667 958 1oi7 (A) 4 288 30.00 0 1 0.577247 -0.66
720 1092 2csu (A) 47 412 13.00 0 0.98 0.345479 0.73
996 1260 1iom (A) 112 357 22.00 0 0.99 0.39717 -0.14
1040 1260 2ifc (A) 152 364 21.00 0 0.98 0.373821 -0.26

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_129655)

Species Accession Gene Product
Arabidopsis thaliana AT3G06650   ATP-citrate lyase B-1
Arabidopsis thaliana AT5G49460   ATP citrate lyase subunit B 2
Brugia malayi Bm1_26245   ATP-citrate synthase
Caenorhabditis elegans CELE_B0365.1   Protein ACLY-2
Caenorhabditis elegans CELE_D1005.1   Protein D1005.1
Dictyostelium discoideum DDB_G0278345   ATP citrate lyase
Drosophila melanogaster Dmel_CG8322   ATP citrate lyase
Homo sapiens ENSG00000131473   ATP citrate lyase
Loa Loa (eye worm) LOAG_00507   ATP-citrate synthase
Mus musculus ENSMUSG00000020917   ATP citrate lyase
Neospora caninum NCLIV_048670   cDNA FLJ55447, highly similar to ATP-citrate synthase (EC 2.3.3.8), related
Oryza sativa 4325777   Os01g0300200
Schistosoma japonicum Sjp_0027590   ATP-citrate synthase, putative
Schistosoma japonicum Sjp_0121000   ATP-citrate synthase, putative
Schistosoma japonicum Sjp_0027600   ko:K01648 ATP citrate (pro-S)-lyase [EC2.3.3.8], putative
Schistosoma mansoni Smp_127730   ATP-citrate synthase
Schmidtea mediterranea mk4.001305.09   ATP-citrate synthase
Schmidtea mediterranea mk4.002624.00  
Schmidtea mediterranea mk4.003068.00   ATP-citrate synthase
Schmidtea mediterranea mk4.001305.08   Putative atp-citrate synthase
Schmidtea mediterranea mk4.000221.08   ATP-citrate synthase
Schmidtea mediterranea mk4.000566.06  
Schmidtea mediterranea mk4.007549.00  
Schmidtea mediterranea mk4.003829.01   ATP-citrate synthase
Toxoplasma gondii TGME49_223840   ATP-citrate lyase, putative

Essentiality

TGME49_223840 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_223840 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus ATP-citrate synthase Compounds References
Homo sapiens ATP citrate lyase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0079 1 1
0.0243 0.5345 0.5
0.0233 1 0.5
0.0097 0.2872 1
0.0097 0.3948 0.3948
0.0169 1 1
0.0077 0.3943 0.4968
0.0064 1 1
0.0106 0.4949 1
0.0097 0.5163 0.5
0.0082 1 1
0.0216 0.5756 1
0.0314 1 1
0.0097 0.4804 0.5
0.0086 0.6672 1
0.0173 1 1
0.0164 1 1
0.0435 1 1
0.016 1 1
0.0159 1 1
0.0211 0.5457 1
0.0097 1 1
0.0443 1 1
0.0061 0.2934 0.5
0.0081 1 1
0.0238 0.2507 1
0.0289 1 1
0.0172 0.3479 1
0.0238 0.2507 1
0.0064 0.7297 1
0.0097 0.3671 0.1542
0.0173 1 1
0.0111 0.263 1
0.0208 1 1
0.016 1 1
0.009 0.6487 1
0.0439 0.2949 0.2949
0.0082 1 1
0.0224 1 1
0.0064 1 1
0.006 0.4057 1
0.0439 0.2949 0.2949
0.0097 1 1
0.0213 1 1
0.0063 1 1
0.0168 0.3649 1
0.0155 0.278 1
0.0097 1 0.5
0.0211 0.5457 1
0.0349 1 1
0.0233 1 0.5
0.0081 1 1
0.0116 0.7321 1
0.0233 1 0.5
0.0169 1 1
0.0097 1 1
0.0082 0.4319 1
0.0221 1 1
0.0079 0.3687 0.1542
0.0254 0.4881 1
0.0222 0.3721 1
0.0116 0.7321 1
0.0167 1 1
0.0064 0.7297 1
0.0443 1 1
0.0266 0.9546 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_223840 (Toxoplasma gondii), ATP-citrate lyase, putative
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