Detailed view for TcCLB.503899.130

Basic information

TDR Targets ID: 32733
Trypanosoma cruzi, glutathione peroxidase-like protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.9036 | Length (AA): 178 | MW (Da): 19596 | Paralog Number: 4

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00255   Glutathione peroxidase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004602   glutathione peroxidase activity  
GO:0055114   oxidation reduction  
GO:0006979   response to oxidative stress  

Metabolic Pathways

Glutathione metabolism (KEGG)
Arachidonic acid metabolism (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 170 2he3 (A) 3 187 31.00 0 1 1.34 -1.71
7 169 3dwv (A) 9 171 73.00 0.000001 1 1.84703 -1.28
11 173 3e0u (A) 14 176 79.00 0.0000056 1 1.80903 -1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3E0U:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_126711)

Species Accession Gene Product
Arabidopsis thaliana AT3G63080   glutathione peroxidase 5
Arabidopsis thaliana AT1G63460   glutathione peroxidase 8
Arabidopsis thaliana AT4G11600   glutathione peroxidase 6
Arabidopsis thaliana AT2G25080   phospholipid hydroperoxide glutathione peroxidase 1
Arabidopsis thaliana AT2G31570   glutathione peroxidase GPx
Arabidopsis thaliana AT2G48150   glutathione peroxidase 4
Arabidopsis thaliana AT4G31870   glutathione peroxidase 7
Arabidopsis thaliana AT2G43350   glutathione peroxidase 3
Brugia malayi Bm1_30395   Probable phospholipid hydroperoxide glutathione peroxidase
Candida albicans CaO19.11915   similar to S. cerevisiae GPX2 (YBR244W) phospholipid hydroperoxide glutathione peroxidase
Candida albicans CaO19.4436   similar to S. cerevisiae GPX2 (YBR244W) phospholipid hydroperoxide glutathione peroxidase
Candida albicans CaO19.7731   one of four potential glutathione peroxidase genes
Candida albicans CaO19.86   one of four potential glutathione peroxidase genes
Candida albicans CaO19.85   one of four potential glutathione peroxidase genes
Candida albicans CaO19.7730   one of four potential glutathione peroxidase genes
Caenorhabditis elegans CELE_R05H10.5   Protein GPX-2
Caenorhabditis elegans CELE_R03G5.5   Protein GPX-7, isoform B
Caenorhabditis elegans CELE_T09A12.2   Protein GPX-6, isoform A
Caenorhabditis elegans CELE_F26E4.12   Protein GPX-1
Cryptosporidium hominis Chro.30063   glutathione peroxidase
Cryptosporidium parvum cgd3_460   glutathione peroxidase
Drosophila melanogaster Dmel_CG12013   CG12013 gene product from transcript CG12013-RC
Escherichia coli b1710   glutathione peroxidase
Echinococcus granulosus EgrG_000374800   phospholipid hydroperoxide glutathione
Homo sapiens 493869   glutathione peroxidase 8 (putative)
Homo sapiens ENSG00000116157   glutathione peroxidase 7
Homo sapiens ENSG00000167468   glutathione peroxidase 4
Leishmania braziliensis LbrM.26.0830   glutathione peroxidase-like protein, putative
Leishmania braziliensis LbrM.26.0820   glutathione peroxidase-like protein, putative
Leishmania donovani LdBPK_260780.1   glutathione peroxidase-like protein, putative
Leishmania infantum LinJ.26.0770   glutathione peroxidase-like protein, putative
Leishmania infantum LinJ.26.0780   glutathione peroxidase-like protein, putative
Leishmania major LmjF.26.0810   type II (glutathione peroxidase-like) tryparedoxin peroxidase
Leishmania major LmjF.26.0800   type II (glutathione peroxidase-like) tryparedoxin peroxidase
Leishmania major LmjF.26.0820   type II (glutathione peroxidase-like) tryparedoxin peroxidase
Leishmania mexicana LmxM.26.0800   type II (glutathione peroxidase-like) tryparedoxin peroxidase
Leishmania mexicana LmxM.26.0810   type II (glutathione peroxidase-like) tryparedoxin peroxidase
Loa Loa (eye worm) LOAG_10617   hypothetical protein
Loa Loa (eye worm) LOAG_10618   glutathione peroxidase
Loa Loa (eye worm) LOAG_05492   hypothetical protein
Mus musculus ensembl-mmu:ENSMUSG00000021760   glutathione peroxidase 8 (putative)
Mus musculus 67305   glutathione peroxidase 7
Mus musculus ENSMUSG00000075706   glutathione peroxidase 4
Neospora caninum NCLIV_048080   Glutathione peroxidase, related
Oryza sativa 4332876   Os03g0358100
Oryza sativa 4336627   Os04g0556300
Oryza sativa 4330235   Os02g0664000
Oryza sativa 4340341   Os06g0185900
Oryza sativa 4350287   Os11g0284900
Onchocerca volvulus OVOC53   Phospholipid hydroperoxide glutathione peroxidase, mitochondrial homolog
Plasmodium berghei PBANKA_0610500   glutathione peroxidase-like thioredoxin peroxidase
Plasmodium falciparum PF3D7_1212000   glutathione peroxidase-like thioredoxin peroxidase
Plasmodium knowlesi PKNH_1234400   glutathione peroxidase-like thioredoxin peroxidase, putative
Plasmodium knowlesi PKNH_1312100   glutathione peroxidase-like thioredoxin peroxidase, putative
Plasmodium vivax PVX_084630   glutathione peroxidase-like thioredoxin peroxidase, putative
Saccharomyces cerevisiae YBR244W   glutathione peroxidase GPX2
Saccharomyces cerevisiae YIR037W   peroxiredoxin HYR1
Saccharomyces cerevisiae YKL026C   glutathione peroxidase GPX1
Schistosoma japonicum Sjp_0218220   ko:K00432 glutathione peroxidase [EC1.11.1.9], putative
Schmidtea mediterranea mk4.015133.00   Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
Schmidtea mediterranea mk4.003305.03  
Trypanosoma brucei gambiense Tbg972.7.1100   trypanothione/tryparedoxin dependent peroxidase 3,glutathione peroxidase-like protein 3, putative
Trypanosoma brucei Tb927.7.1140   trypanothione/tryparedoxin dependent peroxidase 3
Trypanosoma brucei Tb927.7.1130   trypanothione/tryparedoxin dependent peroxidase 2
Trypanosoma congolense TcIL3000_0_04430   trypanothione/tryparedoxin dependent peroxidase 1, cytosolic
Trypanosoma congolense TcIL3000_0_04420   trypanothione/tryparedoxin dependent peroxidase 1, cytosolic
Trypanosoma cruzi TcCLB.507515.10   glutathione peroxidase-like protein, putative
Trypanosoma cruzi TcCLB.503899.110   glutathione peroxidase-like protein, putative
Trypanosoma cruzi TcCLB.507515.4   trypanothione/tryparedoxin dependent peroxidase 2, putative
Trypanosoma cruzi TcCLB.503899.119   trypanothione/tryparedoxin dependent peroxidase 2, putative
Trypanosoma cruzi TcCLB.503899.130   glutathione peroxidase-like protein, putative
Toxoplasma gondii TGME49_266130   peroxiredoxin PRX2
Toxoplasma gondii TGME49_266120   thioredoxin-dependent peroxidase TPX1/2
Trichomonas vaginalis TVAG_248810   glutathione peroxidase, putative

Essentiality

TcCLB.503899.130 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b1710 Escherichia coli non-essential goodall
CELE_F26E4.12 Caenorhabditis elegans embryonic lethal wormbase
CELE_F26E4.12 Caenorhabditis elegans larval arrest wormbase
TGME49_266120 Toxoplasma gondii Essentiality uncertain sidik
TGME49_266130 Toxoplasma gondii Essentiality uncertain sidik
TGME49_266120 Toxoplasma gondii Probably non-essential sidik
TGME49_266130 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Glutathione Peroxidase (1.11.1.9 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma cruzi ( 2 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    TcCLB.503899.130 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma cruzi ( 2 )

Bibliographic References

14 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.503899.130 (Trypanosoma cruzi), glutathione peroxidase-like protein, putative
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