Detailed view for PF3D7_0204500

Basic information

TDR Targets ID: 3313
Plasmodium falciparum, aspartate transaminase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.8007 | Length (AA): 405 | MW (Da): 47005 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00155   Aminotransferase class I and II

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0003824   catalytic activity  
GO:0030170   pyridoxal phosphate binding  
GO:0008483   transaminase activity  
GO:0009058   biosynthetic process  
GO:0006520   amino acid metabolic process  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 405 1vp4 (A) 5 406 13.00 0 1 1.09 -0.68
4 402 1ars () 5 409 32.00 0 1 1.56 -1.8
14 398 7aat (A) 15 404 31.00 0 1 1.54 -2.04
1 405 3k7y (A) 1 405 99.99 0 1 2.2084 -1.2
1 405 3k7y (A) 1 405 99.99 0 1 2.207 -1.18
27 402 2q7w (A) 7 396 32.00 0 1 1.4282 -1.19

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3K7Y:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 24 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, sporozoite, early ring, early schizont, early trophozoite, late ring, late trophozoite, Ring, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 48 hs, merozoite, Oocyst. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Female Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Male Gametocyte. Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_126737)

Species Accession Gene Product
Arabidopsis thaliana AT2G30970   aspartate aminotransferase 1
Arabidopsis thaliana AT5G11520   aspartate aminotransferase 3
Arabidopsis thaliana AT5G19550   aspartate aminotransferase
Arabidopsis thaliana AT1G62800   aspartate aminotransferase 4
Arabidopsis thaliana AT4G31990   aspartate aminotransferase
Babesia bovis BBOV_III010960   aminotransferase, classes I and II family protein
Brugia malayi Bm1_30820   aspartate aminotransferase, identical
Candida albicans CaO19.13666   similar to S. cerevisiae AAT2 (YLR027C) aspartate aminotransferase, cytosolic
Candida albicans CaO19.6287   similar to S. cerevisiae AAT2 (YLR027C) aspartate aminotransferase, cytosolic
Candida albicans CaO19.11037   similar to mammalian mitochondrial aspartate aminotransferase
Candida albicans CaO19.3554   similar to mammalian mitochondrial aspartate aminotransferase
Caenorhabditis elegans CELE_C44E4.3   Protein GOT-2.1
Caenorhabditis elegans CELE_T01C8.5   Protein GOT-1.2
Caenorhabditis elegans CELE_C14F11.1   Protein GOT-2.2, isoform B
Chlamydia trachomatis CT_637   aromatic amino acid aminotransferase
Dictyostelium discoideum DDB_G0268664   aspartate aminotransferase
Dictyostelium discoideum DDB_G0282493   aspartate aminotransferase
Drosophila melanogaster Dmel_CG4233   Glutamate oxaloacetate transaminase 2
Drosophila melanogaster Dmel_CG8430   Glutamate oxaloacetate transaminase 1
Escherichia coli b0928   aspartate aminotransferase, PLP-dependent
Escherichia coli b4054   tyrosine aminotransferase, tyrosine-repressible, PLP-dependent
Echinococcus granulosus EgrG_000778900   aspartate aminotransferase
Echinococcus granulosus EgrG_001134100   aspartate aminotransferase mitochondrial
Echinococcus multilocularis EmuJ_001134100   aspartate aminotransferase, mitochondrial
Echinococcus multilocularis EmuJ_000778900   aspartate aminotransferase
Giardia lamblia GL50803_91056   Aspartate aminotransferase, cytoplasmic
Homo sapiens ENSG00000120053   glutamic-oxaloacetic transaminase 1, soluble
Homo sapiens ENSG00000125166   glutamic-oxaloacetic transaminase 2, mitochondrial
Leishmania braziliensis LbrM.34.0810   aspartate aminotransferase, putative
Leishmania braziliensis LbrM.24.0370   aspartate aminotransferase, putative
Leishmania donovani LdBPK_240370.1   aspartate aminotransferase, putative
Leishmania donovani LdBPK_350840.1   aspartate aminotransferase, putative
Leishmania infantum LinJ.35.0840   aspartate aminotransferase, putative
Leishmania infantum LinJ.24.0370   aspartate aminotransferase, putative
Leishmania major LmjF.24.0370   aspartate aminotransferase, putative
Leishmania major LmjF.35.0820   aspartate aminotransferase, putative
Leishmania mexicana LmxM.34.0820   aspartate aminotransferase, putative
Leishmania mexicana LmxM.24.0370   aspartate aminotransferase, putative
Loa Loa (eye worm) LOAG_10955   aspartate aminotransferase
Mus musculus ensembl-mmu:ENSMUSG00000031672   glutamatic-oxaloacetic transaminase 2, mitochondrial
Mus musculus 102643267   aspartate aminotransferase, cytoplasmic-like
Mus musculus ENSMUSG00000025190   glutamic-oxaloacetic transaminase 1, soluble
Neospora caninum NCLIV_064760   Contig An16c0190, complete genome. (EC 2.6.1.1) (Precursor), related
Oryza sativa 4331017   Os02g0797500
Oryza sativa 4328828   Os02g0236000
Oryza sativa 4341252   Os06g0548000
Oryza sativa 4325621   Os01g0760600
Plasmodium berghei PBANKA_0302300   aspartate aminotransferase, putative
Plasmodium falciparum PF3D7_0204500   aspartate transaminase
Plasmodium knowlesi PKNH_0416400   aspartate aminotransferase, putative
Plasmodium vivax PVX_003655   aspartate aminotransferase, mitochondrial precursor, putative
Plasmodium yoelii PY00897   aminotransferase, classes I and II, putative
Saccharomyces cerevisiae YKL106W   aspartate transaminase AAT1
Saccharomyces cerevisiae YLR027C   aspartate transaminase AAT2
Schistosoma japonicum Sjp_0212390   Aspartate aminotransferase, cytoplasmic, putative
Schistosoma japonicum Sjp_0079220   Aspartate aminotransferase, mitochondrial, putative
Schistosoma japonicum Sjp_0035970   ko:K00813 aspartate aminotransferase [EC2.6.1.1B], putative
Schistosoma mansoni Smp_049150   aspartate aminotransferase
Schistosoma mansoni Smp_064380   branched-chain amino acid aminotransferase
Schmidtea mediterranea mk4.001752.02   Aspartate aminotransferase
Schmidtea mediterranea mk4.001752.01   Aspartate aminotransferase
Schmidtea mediterranea mk4.001208.01  
Schmidtea mediterranea mk4.002005.00   Aspartate aminotransferase, mitochondrial
Schmidtea mediterranea mk4.034998.00   Aspartate aminotransferase, mitochondrial
Trypanosoma brucei gambiense Tbg972.10.4560   aspartate aminotransferase, putative
Trypanosoma brucei gambiense Tbg972.11.5750   aspartate aminotransferase, mitochondrial, putative
Trypanosoma brucei Tb927.10.3660   aspartate aminotransferase
Trypanosoma brucei Tb927.11.5090   aspartate aminotransferase, mitochondrial
Trypanosoma congolense TcIL3000_10_2990   aspartate aminotransferase, putative
Trypanosoma congolense TcIL3000.11.5260   aspartate aminotransferase, mitochondrial, putative
Trypanosoma cruzi TcCLB.503841.70   aspartate aminotransferase, putative
Trypanosoma cruzi TcCLB.503679.10   aspartate aminotransferase, putative
Trypanosoma cruzi TcCLB.510945.70   aspartate aminotransferase, mitochondrial, putative
Toxoplasma gondii TGME49_248600   aspartate aminotransferase
Theileria parva TP02_0046   aspartate aminotransferase, putative
Trichomonas vaginalis TVAG_431780   aspartate aminotransferase, putative
Trichomonas vaginalis TVAG_268020   aspartate aminotransferase, putative
Trichomonas vaginalis TVAG_020800   aspartate aminotransferase, putative

Essentiality

PF3D7_0204500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.3660 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.3660 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.3660 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.3660 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.02.2740 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.2740 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.2740 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.2740 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0928 Escherichia coli non-essential goodall
b4054 Escherichia coli non-essential goodall
CELE_C44E4.3 Caenorhabditis elegans sterile wormbase
CELE_T01C8.5 Caenorhabditis elegans slow growth wormbase
YLR027C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0302300 Plasmodium berghei Slow plasmo
TGME49_248600 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Glutamic-Oxalacetic Transaminase (2.6.1.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

54 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_0204500 (Plasmodium falciparum), aspartate transaminase
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