Detailed view for TcCLB.508175.80

Basic information

TDR Targets ID: 33559
Trypanosoma cruzi, STE/STE11 serine/threonine-protein kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.0369 | Length (AA): 1733 | MW (Da): 188416 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 5

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00498   FHA domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 12 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1206 1680 1fmk () 87 529 22.00 0 1 0.3 0.87
1309 1733 2etr (A) 6 404 20.00 0 1 0.11 0.44
1375 1668 1t46 (A) 581 927 26.00 0 1 0.4 -0.62
987 1122 3pzd (A) 1502 1624 8.00 0 0 0.0654766 -0.71
1074 1116 2ds2 (B) 23 68 19.00 0 0.04 0.165812 -0.71
1213 1673 3i6u (A) 138 492 29.00 0 1 0.0917127 1.31
1294 1709 3pfq (A) 204 637 24.00 0 1 0.306746 0.7
1381 1716 4af3 (A) 76 338 30.00 0.000000012 0.98 0.0118834 1.12
1387 1655 1fot (A) 91 324 29.00 0 1 0.355922 0.16
1387 1715 3c0g (B) 16 324 28.00 0 1 0.458544 -0.22
1387 1722 4lgd (D) 31 476 28.00 0 1 0.438583 -0.18
1465 1600 4ian (A) 762 882 41.00 0.00075 0.99 0.205477 0.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_148284)

Species Accession Gene Product
Leishmania braziliensis LbrM.06.0640   protein kinase, putative
Leishmania donovani LdBPK_060660.1   protein kinase, putative
Leishmania infantum LinJ.06.0660   protein kinase, putative
Leishmania major LmjF.06.0640   protein kinase, putative
Leishmania mexicana LmxM.06.0640   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.7.6000   protein kinase, putative
Trypanosoma brucei Tb927.7.5220   Forkhead Kinase, putative
Trypanosoma cruzi TcCLB.508175.80   STE/STE11 serine/threonine-protein kinase, putative
Trypanosoma cruzi TcCLB.506825.180   STE/STE11 serine/threonine-protein kinase, putative

Essentiality

TcCLB.508175.80 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.5220 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.5220 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.5220 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.5220 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 24.7% 299 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 24.7% 215 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 26.4% 261 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 28.7% 244 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 27.0% 300 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 33.3% 162 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 27.3% 297 aa Compounds References
Patiria pectinifera Cdc2 300 aa 26.6% 305 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0069 0.3067 1
0.0012 0.5 0.5
0.0027 1 0.5
0.0032 0.5 0.5
0.0091 1 0.5
0.0026 0.5 0.5
0.0092 1 0.5
0.0007 0.5 0.5
0.0003 0.5 0.5
0.0061 0.6883 0.5304
0.0039 0.5 0.5
0.0063 0.7244 0.2543
0.0039 0.9485 0.5
0.0033 1 0.5
0.0036 0.5 0.5
0.0022 0.5 0.5
0.0008 0.5 0.5
0.0066 0.3101 0
0.0081 1 0.5
0.0067 0.5 0.5
0.0039 0.5 0.5
0.0042 0.5 0.5
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0059 1 1
0.0098 0.3242 0.3649
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0063 1 0.5
0.0088 0.4477 0.5
0.0056 1 0.5
0.0062 0.6935 0
0.0037 1 0.5
0.0018 0.5 0.5
0.0093 0.8828 0
0.0029 0.5 0.5
0.0011 1 0.5
0.0023 0.5 0.5
0.0059 1 1
0.0064 0.3377 0
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0032 0.5 0.5
0.0059 1 1
0.0016 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TcCLB.508175.80 (Trypanosoma cruzi), STE/STE11 serine/threonine-protein kinase, putative
Title for this comment
Comment