Detailed view for PF3D7_0212200

Basic information

TDR Targets ID: 3386
Plasmodium falciparum, mitochondrial ribosomal protein L12 precursor, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.9744 | Length (AA): 255 | MW (Da): 29547 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00542   Ribosomal protein L7/L12 C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0022625   cytosolic large ribosomal subunit  
GO:0020011   apicoplast  
GO:0005840   ribosome  
GO:0005739   mitochondrion  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
81 255 1dd3 (A) 3 128 36.00 0 1 0.62 0.75
159 255 1dd3 (A) 30 128 37.00 0 1 0.92 -1.15
81 255 1dd3 (A) 3 128 34.00 0 1 0.626875 0.54
158 255 1dd3 (A) 33 128 34.00 0.0000021 1 0.874014 -1.43
183 255 2zjq (5) 52 122 45.00 0 1 0.932875 -1.22

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, late trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile early trophozoite, Oocyst, Male Gametocyte. PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Female Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Sporozoite. Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_126884)

Species Accession Gene Product
Arabidopsis thaliana AT3G27840   50S ribosomal protein L12-2
Arabidopsis thaliana AT1G70190   ribosomal protein L7/L12 domain-containing protein
Arabidopsis thaliana AT4G36420   ribosomal protein L12 family protein
Arabidopsis thaliana AT3G27850   50S ribosomal protein L12-3
Arabidopsis thaliana AT3G27830   50S ribosomal protein L12-1
Arabidopsis thaliana AT4G37660   ribosomal protein L12/ ATP-dependent Clp protease adaptor protein
Babesia bovis BBOV_IV003600   ribosomal protein L7/L12
Babesia bovis BBOV_III003310   hypothetical protein
Brugia malayi Bm1_20670   60S ribosomal protein L12, mitochondrial precursor, putative
Candida albicans CaO19.2275   likely mitochondrial ribosomal large subunit protein similar to S. cerevisiae YGL068W (E. coli L7/L12)
Candida albicans CaO19.9815   likely mitochondrial ribosomal large subunit protein similar to S. cerevisiae YGL068W (E. coli L7/L12)
Caenorhabditis elegans CELE_W09D10.3   Protein MRPL-12
Chlamydia trachomatis CT_316   50S ribosomal protein L7/L12
Dictyostelium discoideum DDB_G0274407   hypothetical protein
Drosophila melanogaster Dmel_CG5012   mitochondrial ribosomal protein L12
Escherichia coli b3986   50S ribosomal subunit protein L7/L12
Echinococcus granulosus EgrG_000891700   39S ribosomal protein L12 mitochondrial
Echinococcus multilocularis EmuJ_000891700   39S ribosomal protein L12, mitochondrial
Homo sapiens ENSG00000262814   mitochondrial ribosomal protein L12
Leishmania braziliensis LbrM.31.3210   ribosomal protein l7/l12-like protein
Leishmania braziliensis LbrM.14.0150   60S ribosomal protein, putative
Leishmania donovani LdBPK_140150.1   60S ribosomal protein, putative
Leishmania donovani LdBPK_312950.1   ribosomal protein l7/l12-like protein
Leishmania infantum LinJ.14.0150   60S ribosomal protein, putative
Leishmania infantum LinJ.31.2950   ribosomal protein l7/l12-like protein
Leishmania major LmjF.31.2850   ribosomal protein l7/l12-like protein
Leishmania major LmjF.14.0150   60S ribosomal protein, putative
Leishmania mexicana LmxM.14.0150   60S ribosomal protein, putative
Leishmania mexicana LmxM.30.2850   ribosomal protein l7/l12-like protein
Mycobacterium leprae ML1895c   PROBABLE 50S RIBOSOMAL PROTEIN L7/L12 RPLL (SA1)
Mus musculus 56282   mitochondrial ribosomal protein L12
Mycobacterium tuberculosis Rv0652   50S ribosomal protein L7/L12 RplL (SA1)
Mycobacterium ulcerans MUL_0742   50S ribosomal protein L7/L12
Neospora caninum NCLIV_066990   hypothetical protein
Oryza sativa 9271252   Os01g0662200
Oryza sativa 4339552   Os05g0554100
Oryza sativa 4343966   Os07g0626100
Oryza sativa 4334016   Os03g0734400
Oryza sativa 4339648   Os05g0568300
Plasmodium berghei PBANKA_0309000   mitochondrial ribosomal protein L12 precursor, putative
Plasmodium berghei PBANKA_1239400   50S ribosomal protein L12, apicoplast, putative
Plasmodium falciparum PF3D7_0212200   mitochondrial ribosomal protein L12 precursor, putative
Plasmodium falciparum PF3D7_0524600   50S ribosomal protein L12, apicoplast, putative
Plasmodium knowlesi PKNH_0409000   ribosomal protein L7/L12, putative
Plasmodium knowlesi PKNH_1008300   50S ribosomal protein L12, apicoplast, putative
Plasmodium vivax PVX_002920   50S ribosomal protein L7/L12, putative
Plasmodium vivax PVX_080020   50S ribosomal protein L12, apicoplast, putative
Plasmodium yoelii PY03559   ribosomal protein L7/L12
Saccharomyces cerevisiae YGL068W   mitochondrial nucleoid protein MNP1
Schistosoma japonicum Sjp_0005850   39S ribosomal protein L12, mitochondrial precursor, putative
Schistosoma mansoni Smp_066740   mitochondrial 60S ribosomal protein L7/L12
Schmidtea mediterranea mk4.000210.00  
Trypanosoma brucei gambiense Tbg972.7.5150   60S ribosomal protein, putative
Trypanosoma brucei gambiense Tbg972.4.5360   ribosomal protein L7/L12, putative
Trypanosoma brucei Tb927.4.5170   ribosomal protein L7/L12, putative
Trypanosoma brucei Tb927.7.4550   Mitochondrial ribosomal protein L7/L12
Trypanosoma congolense TcIL3000_7_3780   60S ribosomal protein-like, putative
Trypanosoma congolense TcIL3000_8_7310   ribosomal protein L7/L12, putative
Trypanosoma congolense TcIL3000_0_46970   ribosomal protein L7/L12, putative
Trypanosoma congolense TcIL3000_0_18300   ribosomal protein L7/L12, putative
Trypanosoma cruzi TcCLB.506605.150   60S ribosomal protein, putative
Trypanosoma cruzi TcCLB.506419.10   ribosomal protein L7/L12, putative
Trypanosoma cruzi TcCLB.511239.140   60S ribosomal protein, putative
Toxoplasma gondii TGME49_251950   ribosomal protein l7/l12 c-terminal domain-containing protein
Toxoplasma gondii TGME49_289230   50S ribosomal protein L12, putative
Treponema pallidum TP0240   50S ribosomal protein L7/L12
Theileria parva TP03_0328   hypothetical protein
Theileria parva TP02_0775   60S ribosomal protein L7/L12, putative
Wolbachia endosymbiont of Brugia malayi Wbm0648   50S ribosomal protein L7/L12

Essentiality

PF3D7_0212200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu664 Mycobacterium tuberculosis essential nmpdr
Tb927.4.5170 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.5170 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.5170 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.5170 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.7.4550 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.4550 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.4550 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.4550 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3986 Escherichia coli essential goodall
CELE_W09D10.3 Caenorhabditis elegans larval arrest wormbase
CELE_W09D10.3 Caenorhabditis elegans slow growth wormbase
YGL068W Saccharomyces cerevisiae inviable yeastgenome
TGME49_251950 Toxoplasma gondii Probably essential sidik
TGME49_289230 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0212200 (Plasmodium falciparum), mitochondrial ribosomal protein L12 precursor, putative
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