Detailed view for PF3D7_0214600

Basic information

TDR Targets ID: 3409
Plasmodium falciparum, serine/threonine protein kinase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.8466 | Length (AA): 1714 | MW (Da): 204289 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 18 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1219 1369 1yrp (A) 10 163 27.00 0 1 0.5 -1.19
1220 1505 2eu9 (A) 154 451 17.00 0 1 0.28 0.16
1466 1713 1cm8 (A) 55 314 14.00 0 0.2 0.1 -0.05
1033 1118 1ux6 (A) 825 923 21.00 0.91 0.01 0.117475 -0.93
1066 1423 4xi2 (A) 223 610 13.00 0.45 0.71 0.111168 1.02
1142 1413 3kvw (A) 0 350 17.00 0.026 0.8 0.152993 0.59
1177 1356 4xi2 (A) 371 532 22.00 0.21 0.91 0.173118 1.08
1213 1371 2vd5 (A) 62 217 26.00 0 0.94 0.312065 0.58
1220 1506 3lij (A) 57 381 18.00 0.0000044 1 0.0687446 0.82
1610 1707 4j8m (A) 289 380 36.00 0.024 0.64 0.388276 -0.12
1610 1650 3udb (C) 158 218 49.00 0.58 0.62 0.352021 -0.11
1211 1501 1kob (A) 34 335 20.00 0.5 1 0.0904877 1.08
1218 1376 2pml (X) 17 196 36.00 0.015 0.78 0.0133881 2.23
1228 1390 3cok (A) 9 201 18.00 0 0.73 0.236612 0.75
1509 1717 2rku (A) 91 305 18.00 0 0.29 0.110341 0.64
1617 1701 4fr4 (A) 180 265 38.00 0.089 0.99 0.36939 0.05
1617 1657 3udb (C) 158 218 49.00 0.59 0.62 0.352823 -0.11
1617 1714 4j8m (A) 289 380 36.00 0.024 0.64 0.388944 -0.12

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile merozoite, sporozoite, late schizont, Sporozoite. PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, early schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 16 hs, late trophozoite. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, Oocyst, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_162068)

Species Accession Gene Product
Plasmodium berghei PBANKA_0311400   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_0214600   serine/threonine protein kinase, putative
Plasmodium knowlesi PKNH_0406200   serine/threonine protein kinase, putative
Plasmodium vivax PVX_002805   calcium-dependent protein kinase 4, putative
Plasmodium yoelii PY06391   Protein kinase domain, putative
Plasmodium yoelii PY03306   hypothetical protein

Essentiality

PF3D7_0214600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0311400 Plasmodium berghei Essential plasmo
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0098 0.3242 0.2614
0.0069 0.3067 1
0.0026 0.5 0.5
0.0056 1 0.5
0.0037 1 0.5
0.0039 0.5 0.5
0.0029 0.5 0.5
0.0012 0.5 0.5
0.0093 0.8828 0
0.0022 0.5 0.5
0.0059 1 1
0.0081 1 0.5
0.0018 0.5 0.5
0.0088 0.4477 0.5
0.0059 1 1
0.0012 0.5 0.5
0.0067 0.5 0.5
0.0091 1 0.5
0.0027 1 0.5
0.0081 0.5 0.5
0.0016 0.5 0.5
0.0032 0.5 0.5
0.0033 1 1
0.0062 0.6935 0
0.0007 0.5 0.5
0.0039 0.9485 0.5
0.0036 0.5 0.5
0.0003 0.5 0.5
0.0059 1 1
0.0004 0.5 0.5
0.0064 0.3377 0
0.0042 0.5 0.5
0.0032 0.5 0.5
0.0061 0.6883 1
0.0033 0.5 0.5
0.0011 1 0.5
0.0008 0.5 0.5
0.0066 0.3101 0
0.0063 0.7244 0.7244
0.0007 0.5 0.5
0.0092 1 0.5
0.0039 0.5 0.5
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0063 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_0214600 (Plasmodium falciparum), serine/threonine protein kinase, putative
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