Detailed view for TcCLB.510719.260

Basic information

TDR Targets ID: 34146
Trypanosoma cruzi, hslVU complex proteolytic subunit, threonine peptidase, Clan T(1), family T1B

Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1162 | Length (AA): 209 | MW (Da): 22881 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00227   Proteasome subunit

Gene Ontology

Mouse over links to read term descriptions.
GO:0009376   HslUV protease complex  
GO:0005839   proteasome core complex  
GO:0004298   threonine endopeptidase activity  
GO:0051603   proteolysis involved in cellular protein catabolic process  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 209 1ryp (D) 8 241 15.00 0 1 1.06 -0.39
20 192 1g3k (A) 1 173 50.00 0 1 1.55 -1.68
20 194 1e94 (A) 1 174 49.00 0 1 1.56 -1.59
8 209 1ryp (C) 21 234 19.00 0.0000029 1 1.17051 0.2
20 192 4hnz (A) 1 173 92.00 0.00000000059 1 2.00375 -1.62

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_130468)

Species Accession Gene Product
Dictyostelium discoideum DDB_G0269282   hypothetical protein
Escherichia coli b3932   peptidase component of the HslUV protease
Leishmania braziliensis LbrM.35.4230   hs1vu complex proteolytic subunit-like,hs1vu complex proteolytic subunit-like, threonine peptidase, Clan T(1), family T1B
Leishmania donovani LdBPK_364180.1   ATP-dependent protease subunit HslV, putative
Leishmania infantum LinJ.36.4180   hs1vu complex proteolytic subunit-like,hs1vu complex proteolytic subunit-like, threonine peptidase, Clan T(1), family T1B
Leishmania major LmjF.36.3990   hs1vu complex proteolytic subunit-like,hs1vu complex proteolytic subunit-like, threonine peptidase, Clan T(1), family T1B
Leishmania mexicana LmxM.36.3990   hs1vu complex proteolytic subunit-like,hs1vu complex proteolytic subunit-like, threonine peptidase, Clan T(1), family T1B
Neospora caninum NCLIV_035050   heat shock protein hslv, putative
Plasmodium berghei PBANKA_1445100   ATP-dependent protease subunit ClpQ, putative
Plasmodium falciparum PF3D7_1230400   ATP-dependent protease subunit ClpQ
Plasmodium knowlesi PKNH_1449800   ATP-dependent protease subunit ClpQ, putative
Plasmodium vivax PVX_124160   ATP-dependent protease subunit ClpQ, putative
Plasmodium yoelii PY03772   hypothetical protein
Trypanosoma brucei gambiense Tbg972.11.11500   hslVU complex proteolytic subunit, putative,hslVU complex proteolytic subunit, threonine peptidase, Clan T(1), family T1B
Trypanosoma brucei Tb927.11.10240   ATP-dependent protease subunit HslV
Trypanosoma congolense TcIL3000.11.10760   ATP-dependent protease subunit HslV, putative
Trypanosoma congolense TcIL3000_0_34240   hslVU complex proteolytic subunit, putative
Trypanosoma cruzi TcCLB.510719.260   hslVU complex proteolytic subunit, threonine peptidase, Clan T(1), family T1B
Trypanosoma cruzi TcCLB.506275.20   ATP-dependent protease subunit HslV, putative
Toxoplasma gondii TGME49_272230   heat shock protein hslv, putative
Wolbachia endosymbiont of Brugia malayi Wbm0722   ATP-dependent protease peptidase subunit

Essentiality

TcCLB.510719.260 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.2000 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.2000 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.2000 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.2000 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3932 Escherichia coli non-essential goodall
TGME49_272230 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0195 0.2531 0.3506
0.0094 0.2614 0.276
0.0096 0.2611 0.2775

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.510719.260 (Trypanosoma cruzi), hslVU complex proteolytic subunit, threonine peptidase, Clan T(1), family T1B
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