Detailed view for TcCLB.506229.50

Basic information

TDR Targets ID: 34434
Trypanosoma cruzi, nucleoside phosphatase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.9334 | Length (AA): 636 | MW (Da): 70737 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01150   GDA1/CD39 (nucleoside phosphatase) family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016787   hydrolase activity  

Metabolic Pathways

Starch and sucrose metabolism (KEGG)
Folate biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
205 456 1u6z (A) 16 227 21.00 0.0000000079 0.74 0.41 0.73
48 190 5xg2 (A) 374 743 16.00 0 0.07 0.377243 -0.2
175 628 3agr (A) 16 580 21.00 0 1 0.696236 0.64
199 633 5u7x (F) 5 404 32.00 0 1 0.918862 -0.16
199 631 3zx3 (A) 47 460 27.00 0 1 0.948718 -0.29
201 625 5u7w (A) 7 399 35.00 0 1 0.880539 0.01

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127709)

Species Accession Gene Product
Arabidopsis thaliana AT3G04080   apyrase 1
Arabidopsis thaliana AT5G18280   apyrase 2
Candida albicans CaO19.7394   guanosine diphosphatase
Caenorhabditis elegans CELE_K08H10.4   Protein UDA-1
Drosophila melanogaster Dmel_CG3059   CG3059 gene product from transcript CG3059-RD
Homo sapiens 957   ectonucleoside triphosphate diphosphohydrolase 5
Homo sapiens ENSG00000197586   ectonucleoside triphosphate diphosphohydrolase 6 (putative)
Leishmania braziliensis LbrM.15.0030   ATP diphosphohydrolase
Leishmania braziliensis LbrM.10.0170   nucleoside phosphatase, putative,guanosine diphosphatase, putative
Leishmania donovani LdBPK_150030.1   ATP diphosphohydrolase
Leishmania donovani LdBPK_100150.1   nucleoside phosphatase, putative
Leishmania infantum LinJ.10.0150   nucleoside phosphatase, putative,guanosine diphosphatase, putative
Leishmania infantum LinJ.15.0030   ATP diphosphohydrolase
Leishmania major LmjF.15.0030   ATP diphosphohydrolase
Leishmania major LmjF.10.0170   nucleoside phosphatase, putative,guanosine diphosphatase, putative
Leishmania mexicana LmxM.15.0030   nucleoside diphosphatase, putative
Leishmania mexicana LmxM.10.0170   nucleoside phosphatase, putative,guanosine diphosphatase, putative
Mus musculus ENSMUSG00000033068   ectonucleoside triphosphate diphosphohydrolase 6
Mus musculus ENSMUSG00000021236   ectonucleoside triphosphate diphosphohydrolase 5
Neospora caninum NCLIV_060550   Ectonucleoside triphosphate diphosphohydrolase 2, related
Oryza sativa 4350421   Os11g0440200
Oryza sativa 4350420   Os11g0439600
Oryza sativa 4349645   Os11g0126400
Oryza sativa 4351367   Os12g0123500
Oryza sativa 4344315   Os07g0682800
Saccharomyces cerevisiae YEL042W   Gda1p
Schistosoma japonicum Sjp_0214380   ko:K01511 nucleoside-diphosphatase [EC3.6.1.6], putative
Schistosoma mansoni Smp_081450   adenosine diphosphatase
Schmidtea mediterranea mk4.003992.02  
Schmidtea mediterranea mk4.010067.00  
Schmidtea mediterranea mk4.000295.05   Nucleoside-diphosphatase uda-1
Schmidtea mediterranea mk4.001744.00  
Schmidtea mediterranea mk4.003743.01  
Schmidtea mediterranea mk4.002538.04  
Schmidtea mediterranea mk4.003743.03  
Trypanosoma brucei gambiense Tbg972.8.3510   nucleoside phosphatase, putative,guanosine diphosphatase, putative
Trypanosoma brucei gambiense Tbg972.7.1920   nucleoside diphosphatase, putative
Trypanosoma brucei Tb927.8.3800   nucleoside phosphatase, putative
Trypanosoma brucei Tb927.7.1930   nucleoside diphosphatase, putative
Trypanosoma congolense TcIL3000_0_35580   nucleoside diphosphatase, putative
Trypanosoma congolense TcIL3000_8_3670   nucleoside phosphatase, putative
Trypanosoma cruzi TcCLB.506229.50   nucleoside phosphatase, putative
Toxoplasma gondii TGME49_307800   GDA1/CD39 (nucleoside phosphatase) family protein

Essentiality

TcCLB.506229.50 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.3800 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.3800 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.3800 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.3800 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.7.1930 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.1930 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.1930 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.1930 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_307800 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
catalytic activity (GO:0003824) decreased (PATO:0000468) trypomastigote (BTO:0001398) inferred from animal model system (ECO:0000179) Trypanosoma cruzi 377156   377555   377687   378821   580635   912688  
Annotator: millerja@u.washington.edu. Comment: Note: GeneBank ID is AY540630.1; included 3 hits (98% or better homology in BLAST search) to Tc genes in column A. Note: drugs also appear to reduce infectivity. Note: unable to find ARL 67156 in TDR Targets.. References: 19255624

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Nucleoside triphosphate diphosphohydrolase 3 529 aa 23.2% 475 aa Compounds References
Bos taurus Ectonucleoside triphosphate diphosphohydrolase 1 513 aa 25.8% 477 aa Compounds References
Trypanosoma cruzi nucleoside phosphatase, putative 246 aa 98.8% 246 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0042 0.4127 1
0.0087 0.5 0.5
0.0087 0.2929 1
0.0056 0.4121 0.9554

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Reagent:
  • Target Type Source Notes
    TcCLB.506229.50 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Trypanosoma cruzi ( 1 )

Bibliographic References

213 literature references were collected for this gene.

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Gene identifier TcCLB.506229.50 (Trypanosoma cruzi), nucleoside phosphatase, putative
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