Detailed view for TcCLB.509599.130

Basic information

TDR Targets ID: 34685
Trypanosoma cruzi, NEK family Serine/threonine-protein kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.9616 | Length (AA): 495 | MW (Da): 54960 | Paralog Number: 3

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00169   PH domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0005524   ATP binding  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 369 1kob (A) 23 375 21.00 0 1 0.86 0.02
1 307 2g15 (A) 1047 1345 20.00 0 1 0.78 -0.53
31 488 1omw (A) 189 650 21.00 0 1 1.06 0.39
115 296 1t46 (A) 668 923 26.00 0.00000000029 1 0.81 -1.16
1 295 3cbl (A) 520 812 21.00 0 1 0.78286 0.2
31 300 3bhy (A) 11 276 29.00 0 1 0.795355 -0.33
31 489 3pvu (A) 189 651 19.00 0 1 0.951573 1.12
31 489 3v5w (A) 189 651 19.00 0 1 1.04757 0.67
123 297 5cem (A) 165 336 33.00 0.0000000000064 1 0.747835 -0.72
148 297 4x7q (A) 144 284 42.00 0 1 0.69433 -0.59

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_135887)

Species Accession Gene Product
Leishmania braziliensis LbrM.35.1660   hypothetical protein, conserved,nima-related protein kinase, putative
Leishmania donovani LdBPK_361580.1   hypothetical protein, conserved
Leishmania infantum LinJ.36.1580   hypothetical protein, conserved,nima-related protein kinase, putative
Leishmania major LmjF.36.1520   hypothetical protein, conserved,nima-related protein kinase, putative
Leishmania mexicana LmxM.36.1520   hypothetical protein, conserved,nima-related protein kinase, putative
Trypanosoma brucei gambiense Tbg972.10.7220   protein kinase, putative,(OTHER) NEK family, HsNEK1-like
Trypanosoma brucei gambiense Tbg972.10.7270   protein kinase, putative
Trypanosoma brucei Tb11.v5.0190   protein kinase, putative
Trypanosoma brucei Tb927.10.5930   NEK family Serine/threonine-protein kinase, putative
Trypanosoma brucei Tb927.10.5940   NEK family Serine/threonine-protein kinase, putative
Trypanosoma congolense TcIL3000_10_4970   protein kinase, putative
Trypanosoma cruzi TcCLB.509599.150   Serine/threonine-protein kinase NEK17, putative
Trypanosoma cruzi TcCLB.509599.130   NEK family Serine/threonine-protein kinase, putative
Trypanosoma cruzi TcCLB.506327.90   Serine/threonine-protein kinase NEK17, putative
Trypanosoma cruzi TcCLB.463451.10   NEK family Serine/threonine-protein kinase, putative

Essentiality

TcCLB.509599.130 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.5950 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.5950 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.5950 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.5950 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.5940 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.5940 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.5940 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.5940 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Xenopus laevis Aurora kinase B-A 361 aa 26.1% 307 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 25.7% 307 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 26.8% 280 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 32.5% 277 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0023 0.5 0.5
0.0011 1 0.5
0.0029 0.5 0.5
0.0093 0.8828 0
0.0018 0.5 0.5
0.0037 1 0.5
0.0062 0.6935 0
0.0016 0.5 0.5
0.0032 0.5 0.5
0.0059 1 1
0.0007 0.5 0.5
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0059 1 1
0.0064 0.3377 0
0.0012 0.5 0.5
0.0098 0.3242 0.3649
0.0056 1 0.5
0.0088 0.4477 0.5
0.0063 1 0.5
0.0016 0.5 0.5
0.0039 0.9485 0.5
0.0033 1 0.5
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0059 1 1
0.0039 0.5 0.5
0.0067 0.5 0.5
0.0042 0.5 0.5
0.0081 1 0.5
0.0066 0.3101 0
0.0008 0.5 0.5
0.0022 0.5 0.5
0.0036 0.5 0.5
0.0026 0.5 0.5
0.0091 1 0.5
0.0032 0.5 0.5
0.0012 0.5 0.5
0.0027 1 0.5
0.0069 0.3067 1
0.0063 0.7244 0.2543
0.0039 0.5 0.5
0.0061 0.6883 0.5304
0.0003 0.5 0.5
0.0092 1 0.5
0.0007 0.5 0.5

Assayability

Assay information

  • Assay for Protein Kinase C (2.7.1.37 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

144 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.509599.130 (Trypanosoma cruzi), NEK family Serine/threonine-protein kinase, putative
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