Detailed view for TcCLB.511859.60

Basic information

TDR Targets ID: 35162
Trypanosoma cruzi, subtilisin peptidase
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.3541 | Length (AA): 1408 | MW (Da): 158016 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00082   Subtilase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0004252   serine-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
128 526 3afg (A) 61 397 25.00 0 1 0.238181 0.73
170 492 1thm (A) 6 237 34.00 0.0000025 1 0.172203 0.4
428 495 4i0w (B) 452 509 52.00 0.0045 0.11 0.168095 0.74

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_141540)

Species Accession Gene Product
Leishmania braziliensis LbrM.13.0860   subtilisin-like serine peptidase,serine peptidase, clan SB, family S8-like protein
Leishmania donovani LdBPK_130940.1   subtilisin peptidase
Leishmania infantum LinJ.13.0940   subtilisin-like serine peptidase,serine peptidase, clan SB, family S8-like protein
Leishmania infantum LinJ.13.0930   subtilisin-like serine peptidase,serine peptidase, clan SB, family S8-like protein
Leishmania major LmjF.13.1040   subtilisin-like serine peptidase
Leishmania mexicana LmxM.13.1040   subtilisin-like serine peptidase,serine peptidase, clan SB, family S8-like protein
Trypanosoma brucei gambiense Tbg972.11.4340   subtilisin-like serine peptidase,serine peptidase, clan SB, family S8-like protein, putative
Trypanosoma brucei Tb927.11.3780   subtilisin peptidase
Trypanosoma congolense TcIL3000.11.3740   subtilisin peptidase
Trypanosoma congolense TcIL3000_0_54310   subtilisin peptidase
Trypanosoma cruzi TcCLB.511859.60   subtilisin peptidase
Trypanosoma cruzi TcCLB.511045.40   serine peptidase, clan SB, family S8-like protein

Essentiality

TcCLB.511859.60 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.1280 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.1280 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.1280 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.1280 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Bacillus lentus Subtilisin 269 aa 28.0% 218 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.511859.60 (Trypanosoma cruzi), subtilisin peptidase
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