Detailed view for PF3D7_0304500

Basic information

TDR Targets ID: 3546
Plasmodium falciparum, 1-cys-glutaredoxin-like protein-1
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.6326 | Length (AA): 171 | MW (Da): 19920 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00462   Glutaredoxin

Gene Ontology

Mouse over links to read term descriptions.
GO:0015038   glutathione disulfide oxidoreductase activity  
GO:0015035   protein disulfide oxidoreductase activity  
GO:0009055   electron carrier activity  
GO:0045454   cell redox homeostasis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
66 171 1z7p (A) 3 105 22.00 0 1 1 -2.41
70 171 1yka (A) 1 101 39.00 0 1 1.3 -2.27
74 171 2fls (A) 17 111 23.00 0 1 1 -2.76
50 171 2mmz (A) 1 139 41.00 0 1 1.09465 -0.78
67 171 3ipz (A) 65 168 41.00 0 1 1.30414 -2.03

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Oocyst, Sporozoite. Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, late trophozoite. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont, early trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, Female Gametocyte, Male Gametocyte. Otto TD Lasonder E
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_127295)

Species Accession Gene Product
Arabidopsis thaliana AT3G15660   glutaredoxin 4
Arabidopsis thaliana AT3G54900   CAX interacting protein 1
Babesia bovis BBOV_IV010730   hypothetical protein
Brugia malayi Bm1_28350   Hypothetical UPF0055 protein YPL059w
Candida albicans CaO19.10298   glutaredoxin-like orf similar to S. cerevisiae GRX5 (YPL059W) mitochondrial matrix enzyme
Candida albicans CaO19.2782   glutaredoxin-like orf similar to S. cerevisiae GRX5 (YPL059W) mitochondrial matrix enzyme
Caenorhabditis elegans CELE_Y49E10.2   Protein GLRX-5
Dictyostelium discoideum DDB_G0274657   glutaredoxin-related family protein
Drosophila melanogaster Dmel_CG14407   CG14407 gene product from transcript CG14407-RA
Escherichia coli b1654   glutaredoxin-4
Giardia lamblia GL50803_2013   Glutaredoxin-related protein
Homo sapiens 51218   glutaredoxin 5
Leishmania braziliensis LbrM.01.0140   glutaredoxin-like protein
Leishmania donovani LdBPK_010110.1   glutaredoxin-like protein
Leishmania infantum LinJ.01.0110   glutaredoxin-like protein
Leishmania major LmjF.01.0110   glutaredoxin-like protein
Leishmania mexicana LmxM.01.0110   glutaredoxin-like protein
Loa Loa (eye worm) LOAG_09509   hypothetical protein
Mus musculus ENSMUSG00000021102   glutaredoxin 5 homolog (S. cerevisiae)
Neospora caninum NCLIV_037620   Glutaredoxin, related
Neospora caninum NCLIV_065200   hypothetical protein, conserved
Oryza sativa 4334801   Os03g0851200
Oryza sativa 4327130   Os01g0174900
Oryza sativa 4327794   Os01g0530400
Plasmodium berghei PBANKA_0403100   1-cys-glutaredoxin-like protein-1, putative
Plasmodium berghei PBANKA_1219700   glutaredoxin-like protein
Plasmodium falciparum PF3D7_0709200   glutaredoxin-like protein
Plasmodium falciparum PF3D7_0304500   1-cys-glutaredoxin-like protein-1
Plasmodium knowlesi PKNH_0838600   1-cys-glutaredoxin-like protein-1, putative
Plasmodium knowlesi PKNH_0107800   glutaredoxin-like protein
Plasmodium vivax PVX_119350   1-cys-glutaredoxin-like protein-1, putative
Plasmodium vivax PVX_087990   glutaredoxin-like protein
Plasmodium yoelii PY05059   glutaredoxin like-protein-related
Plasmodium yoelii PY03169   1-cys-glutaredoxin-like protein-1
Plasmodium yoelii PY07597   Plasmodium falciparum CG6
Saccharomyces cerevisiae YPL059W   monothiol glutaredoxin GRX5
Schmidtea mediterranea mk4.000071.06   Glutaredoxin-related protein 5, mitochondrial
Trypanosoma brucei gambiense Tbg972.9.1840   glutaredoxin-like protein, putative
Trypanosoma brucei Tb11.v5.0904   glutaredoxin-like protein, putative
Trypanosoma brucei Tb927.9.3590   monothiol glutaredoxin, putative
Trypanosoma congolense TcIL3000_9_1080   monothiol glutaredoxin, Grx4 family, putative
Trypanosoma congolense TcIL3000_0_30490   monothiol glutaredoxin, putative
Trypanosoma cruzi TcCLB.506893.80   monothiol glutaredoxin, putative
Trypanosoma cruzi TcCLB.508501.230   monothiol glutaredoxin, putative
Toxoplasma gondii TGME49_268730   glutaredoxin-related protein
Toxoplasma gondii TGME49_247580   glutaredoxin domain-containing protein
Theileria parva TP01_0735   hypothetical protein, conserved
Theileria parva TP04_0177   1-Cys-glutaredoxin-like protein, putative
Wolbachia endosymbiont of Brugia malayi Wbm0676   glutaredoxin-like protein

Essentiality

PF3D7_0304500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.2210 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.160.2210 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.160.2210 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.2210 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y49E10.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y49E10.2 Caenorhabditis elegans slow growth wormbase
PBANKA_1219700 Plasmodium berghei Dispensable plasmo
TGME49_268730 Toxoplasma gondii Essentiality uncertain sidik
TGME49_247580 Toxoplasma gondii Essentiality uncertain sidik
TGME49_268730 Toxoplasma gondii Probably essential sidik
TGME49_247580 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0304500 (Plasmodium falciparum), 1-cys-glutaredoxin-like protein-1
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