Detailed view for TcCLB.503677.10

Basic information

TDR Targets ID: 35768
Trypanosoma cruzi, phosphomannose isomerase, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2273 | Length (AA): 408 | MW (Da): 45462 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01238   Phosphomannose isomerase type I

Gene Ontology

Mouse over links to read term descriptions.
GO:0009298   GDP-mannose biosynthetic process  
GO:0008270   zinc ion binding  
GO:0004476   mannose-6-phosphate isomerase activity  
GO:0005975   carbohydrate metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 390 1pmi () 4 424 35.00 0 1 1.38 -0.81
1 397 2wfp (A) 0 388 30.00 0 1 1.30224 -0.37
3 388 1pmi (A) 5 424 33.00 0 1 1.31528 -0.37
4 368 1pmi (A) 6 396 37.00 0 1 1.27541 -0.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127555)

Species Accession Gene Product
Arabidopsis thaliana AT1G67070   mannose-6-phosphate isomerase
Arabidopsis thaliana AT3G02570   mannose-6-phosphate isomerase
Babesia bovis BBOV_III009640   phosphomannose isomerase type I family protein
Brugia malayi Bm1_52850   mannose-6-phosphate isomerase, class I family protein
Brugia malayi Bm1_32455   mannose-6-phosphate isomerase, class I family protein
Candida albicans CaO19.8968   mannose-6-phosphate isomerase capable of functionally substituting for S. cerevisiae PMI40 (YER003C)
Candida albicans CaO19.1390   mannose-6-phosphate isomerase capable of functionally substituting for S. cerevisiae PMI40 (YER003C)
Caenorhabditis elegans CELE_ZK632.4   Protein ZK632.4
Caenorhabditis elegans CELE_C05C8.7   Protein C05C8.7
Cryptosporidium hominis Chro.80224   hypothetical protein
Cryptosporidium parvum cgd8_1920   mannose-6-phosphate isomerase
Dictyostelium discoideum DDB_G0284685   mannose-6-phosphate isomerase
Drosophila melanogaster Dmel_CG8417   CG8417 gene product from transcript CG8417-RA
Escherichia coli b1613   mannose-6-phosphate isomerase
Echinococcus granulosus EgrG_000517700   mannose 6 phosphate isomerase
Entamoeba histolytica EHI_162210   phosphomannose isomerase, putative
Echinococcus multilocularis EmuJ_000517700   mannose 6 phosphate isomerase
Homo sapiens ENSG00000178802   mannose phosphate isomerase
Leishmania braziliensis LbrM.32.1750   phosphomannose isomerase
Leishmania donovani LdBPK_321660.1   phosphomannose isomerase
Leishmania infantum LinJ.32.1660   phosphomannose isomerase
Leishmania major LmjF.32.1580   phosphomannose isomerase
Leishmania mexicana LmxM.31.1580   phosphomannose isomerase, putative
Loa Loa (eye worm) LOAG_01312   mannose-6-phosphate isomerase
Loa Loa (eye worm) LOAG_01345   mannose-6-phosphate isomerase
Mycobacterium leprae ML0765   probable mannose-6-phosphate isomerase ManA (PHOSPHOMANNOSE ISOMERASE) (PHOSPHOMANNOISOMERASE) (PMI) (PHOSPHOHEXOISOMERASE) (PHO
Mus musculus ENSMUSG00000032306   mannose phosphate isomerase
Mycobacterium tuberculosis Rv3255c   Probable mannose-6-phosphate isomerase ManA (phosphomannose isomerase) (phosphomannoisomerase) (PMI) (phosphohexoisomerase) (pho
Mycobacterium ulcerans MUL_2600   mannose-6-phosphate isomerase ManA
Neospora caninum NCLIV_026860   hypothetical protein
Oryza sativa 4325991   Os01g0127900
Oryza sativa 4346957   Os09g0389000
Plasmodium berghei PBANKA_1228400   mannose-6-phosphate isomerase, putative
Plasmodium falciparum PF3D7_0801800   mannose-6-phosphate isomerase, putative
Plasmodium knowlesi PKNH_0117100   mannose-6-phosphate isomerase, putative
Plasmodium vivax PVX_093650   mannose-6-phosphate isomerase, putative
Plasmodium yoelii PY03463   Phosphomannose isomerase type I, putative
Saccharomyces cerevisiae YER003C   mannose-6-phosphate isomerase PMI40
Schistosoma japonicum Sjp_0210490   ko:K01809 mannose-6-phosphate isomerase [EC5.3.1.8], putative
Schistosoma mansoni Smp_089590   mannose-6-phosphate isomerase
Schmidtea mediterranea mk4.000517.14   Mannose-6-phosphate isomerase
Schmidtea mediterranea mk4.036709.00   Mannose-6-phosphate isomerase
Trypanosoma brucei gambiense Tbg972.11.16590   phosphomannose isomerase, putative
Trypanosoma brucei Tb927.11.14780   phosphomannose isomerase
Trypanosoma congolense TcIL3000.11.15070   phosphomannose isomerase, putative
Trypanosoma cruzi TcCLB.511717.90   phosphomannose isomerase, putative
Trypanosoma cruzi TcCLB.503677.10   phosphomannose isomerase, putative
Toxoplasma gondii TGME49_260140   phosphomannose isomerase type I protein
Theileria parva TP04_0879   mannose-6-phosphate isomerase, putative
Trichomonas vaginalis TVAG_343390   mannose-6-phosphate isomerase, putative

Essentiality

TcCLB.503677.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.6410 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.6410 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.6410 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.6410 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C05C8.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_C05C8.7 Caenorhabditis elegans larval arrest wormbase
CELE_C05C8.7 Caenorhabditis elegans larval lethal wormbase
CELE_C05C8.7 Caenorhabditis elegans slow growth wormbase
CELE_C05C8.7 Caenorhabditis elegans sterile wormbase
YER003C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1228400 Plasmodium berghei Slow plasmo
TGME49_260140 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens mannose phosphate isomerase Compounds References
Candida albicans mannose-6-phosphate isomerase capable of functionally substituting for S. cerevisiae PMI40 (YER003C) Compounds References
Saccharomyces cerevisiae mannose-6-phosphate isomerase PMI40 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.122 0.6801 0.6801
0.0117 0.7019 1
0.0117 0.263 1
0.0117 0.3252 0.5
0.0207 1 0.5
0.0191 0.3203 0.32
0.0129492 1 1
0.0222 0.6893 0.5
0.0107316 1 1
0.0117 0.2787 0.4368
0.0109 0.2844 0.3864
0.0302 0.4136 0.4395
0.01 1 1
0.1806 0.6683 0.6673
0.0235 0.2649 0.5611
0.0117 0.2677 0.5
0.0768 0.5578 0.595
0.0395 0.2634 0.9261
0.0694 0.8547 1
0.0736 0.7389 0.7653
0.0695 0.3228 1
0.0424 0.4977 1
0.1394 0.7918 0.7915
0.12 0.6098 0.6097
0.0113 0.3326 1
0.1296 0.5744 0.5968
0.0587 0.5881 0.6468
0.0995 0.572 0.5719
0.0204 0.2538 0.2501
0.0301 0.4174 1
0.0497 0.5256 0.5465
0.0294 0.2857 0.4517
0.1028 0.361 1
0.1103 0.6212 0.6212
0.0117 0.2985 0.2985
0.1748 0.4286 0.7019
0.0689 0.6191 0.6385
0.1296 0.5744 0.5968
0.0605 0.2665 0.4353
0.0117 0.3326 1
0.0405 0.4326 1
0.0802 0.3732 1
0.1121 0.5492 0.5491
0.0777 0.7036 0.7249
0.0117 1 1
0.1103 0.6212 0.6212
0.0117 1 1
0.0687 0.4898 0.5257
0.0201 1 0.5
0.1711 0.6608 0.6604
0.2057 0.5761 0.5752
0.01 0.3531 0.9018
0.0484 0.5888 0.7066
0.0587 0.623 0.6468
0.0894 0.6845 0.6845
0.1811 0.7374 0.737
0.0117 0.3952 1
0.0783 0.5998 0.5987
0.1491 0.5669 0.5667
0.0192 0.3053 0.3008
0.0117 0.3291 0.2509
0.0901 0.5168 1
0.1742 0.3685 0.7011
0.1806 0.6683 0.6673
0.0919 0.4232 1
0.0329 0.3558 1
0.2163 0.2668 0.3977
0.019 0.2614 0.4027
0.0096 1 1
0.0117 1 1
0.0094 0.2848 0.3864
0.1296 0.5744 0.5968
0.0798082 0.905042 1
0.0107 0.2615 0.8549
0.1082 0.6531 0.8418
0.0117 1 1
0.1075 0.4967 0.4951
0.2154 0.6817 0.6809
0.1478 0.6043 0.6042
0.0313 1 0.5
0.0117 0.275 1
0.0097 1 0.5
0.1608 0.5693 0.5688
0.0296 1 1
0.0117 0.5651 1
0.1381 0.7024 0.7021
0.0212 1 0.5
0.0096 0.2562 0.4707
0.039 0.449 0.5874
0.0989 0.5641 0.5641
0.0117 1 0.5
0.0095 1 0.5
0.0117 1 1
0.0737 0.7383 0.7667
0.0383 0.475 1
0.0384 0.4716 1
0.2057 0.5761 0.5752
0.1075 0.4967 0.4951
0.0299 0.3877 0.386
0.0117 0.2899 0.2624
0.199 0.6122 0.6122
0.0289 0.3592 0.355
0.0099 0.3291 0.0207
0.0508 0.351 0.7809
0.0192 0.2667 0.4186
0.0427 0.5694 1
0.0591 0.5467 0.5458
0.0117 0.2769 0.2769
0.0586 0.5751 0.598
0.0170735 0.480375 1
0.1371 0.6335 0.6335
0.1081 0.6151 0.6149
0.0205 1 0.5
0.1197 0.917 0.9169
0.0103 0.2747 0.5
0.22047 1 1
0.0115 0.2898 0.3864
0.01 0.3531 0.9018
0.03 0.3201 0.6709
0.0794 0.3737 1
0.0992 0.7494 0.7492
0.0388 0.3576 0.371
0.0919 0.8207 0.8204
0.0989 0.5641 0.5641
0.0194 1 0.5
0.0207 1 0.5
0.0099 0.3291 0.0207
0.0887 0.5095 0.5095
0.1434 0.6491 0.6491
0.0117 1 1
0.0117 0.3589 0.3744
0.0413 0.5662 1
0.0405 0.7742 1
0.1508 0.572 0.5705
0.0117 0.2895 0.415
0.0786 0.8057 1
0.0112 0.282 0.3864
0.0405 0.4326 1
0.0289 0.363 0.3629
0.0491 0.5118 0.5964
0.0689 0.6366 0.6354
0.0485 0.4561 0.4754
0.0215 1 0.5
0.1748 0.4286 0.7019
0.01 1 1
0.0117 0.2803 1
0.1186 0.6109 0.7851
0.0098 0.7866 0
0.0797 0.8868 0.9063
0.0685 0.6354 0.6347
0.0379 0.5478 1
0.247536 1 1
0.1003 1 1
0.0605 0.2665 0.4353
0.0117 1 1
0.0102 1 0.5
0.0491 0.5769 0.7665
0.0736 0.7389 0.7653
0.1422 0.6358 0.6357
0.0301 0.3672 1
0.0192 0.3837 1
0.14 0.6146 0.6387
0.0405 0.4326 1
0.0484 0.5888 0.7066
0.1748 0.4286 0.7019
0.0117 1 1
0.0117 0.3156 0.3767
0.2163 0.2668 0.3977
0.2032 0.7526 0.7521
0.0117 1 1
0.0877 0.6373 0.7998
0.0489 0.4671 0.4858
0.0416 0.5514 1
0.0605 0.2665 0.4353
0.111 0.731 0.7309
0.0292 0.3091 1
0.0117 1 1
0.0114 0.282 1
0.0117 1 1
0.1212 0.5883 0.5984
0.0405 0.5045 0.5289
0.0497 0.5008 0.5555
0.1942 0.7862 0.7856
0.0117 0.3718 1
0.1147 0.2599 1
0.0387 0.3503 0.3644
0.1103 0.6212 0.6212
0.0393 0.418 0.4334
0.0097 0.304 1
0.0095 0.3517 0.4835
0.0786 0.8057 1
0.1296 0.5744 0.5968
0.1008 1 1
0.1193 0.3321 1
0.2057 0.5761 0.5752
0.1628 0.4379 0.7157
0.03 0.3484 0.4051

Assayability

Assay information

  • Assay for Phosphomannose Isomerase (5.3.1.8 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.503677.10 (Trypanosoma cruzi), phosphomannose isomerase, putative
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