Detailed view for TcCLB.503477.20

Basic information

TDR Targets ID: 36694
Trypanosoma cruzi, transaldolase, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2603 | Length (AA): 331 | MW (Da): 36447 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00923   Transaldolase/Fructose-6-phosphate aldolase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0004801   transaldolase activity  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  
GO:0006098   pentose-phosphate shunt  
GO:0005975   carbohydrate metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 331 1onr (A) 3 317 57.00 0 1 1.62 -1.69
2 331 2cwn (A) 13 330 53.00 0 1 1.59 -1.75
2 331 3cwn (A) 3 317 57.00 0 1 1.75718 -1.61
2 331 1f05 (A) 13 330 53.00 0 1 1.71618 -1.56

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127238)

Species Accession Gene Product
Arabidopsis thaliana AT1G12230   transaldolase-like protein
Brugia malayi Bm1_04195   Transaldolase
Candida albicans CaO19.11849   Transaldolase
Candida albicans CaO19.4371   Transaldolase
Caenorhabditis elegans CELE_Y24D9A.8   Protein Y24D9A.8, isoform B
Chlamydia trachomatis CT_313   transaldolase
Dictyostelium discoideum DDB_G0280909   hypothetical protein
Drosophila melanogaster Dmel_CG2827   CG2827 gene product from transcript CG2827-RA
Escherichia coli b3946   fructose-6-phosphate aldolase 2
Escherichia coli b2464   transaldolase A
Escherichia coli b0008   transaldolase B
Escherichia coli b0825   fructose-6-phosphate aldolase 1
Echinococcus granulosus EgrG_000092800   transaldolase
Echinococcus multilocularis EmuJ_000092800   transaldolase
Homo sapiens ENSG00000177156   transaldolase 1
Leishmania braziliensis LbrM.16.0750   transaldolase, putative
Leishmania donovani LdBPK_160760.1   transaldolase, putative
Leishmania infantum LinJ.16.0760   transaldolase, putative
Leishmania major LmjF.16.0760   transaldolase, putative
Leishmania mexicana LmxM.16.0760   transaldolase, putative
Loa Loa (eye worm) LOAG_02901   transaldolase
Mus musculus ENSMUSG00000025503   transaldolase 1
Neospora caninum NCLIV_030290   transaldolase, putative
Oryza sativa 4344683   Os08g0154300
Onchocerca volvulus OVOC3446   Transaldolase homolog
Saccharomyces cerevisiae YGR043C   sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate transaldolase NQM1
Saccharomyces cerevisiae YLR354C   sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate transaldolase TAL1
Schistosoma japonicum Sjp_0213130   ko:K00616 transaldolase [EC2.2.1.2], putative
Schistosoma mansoni Smp_070600   transaldolase
Schmidtea mediterranea mk4.008991.00  
Schmidtea mediterranea mk4.010083.00   Probable transaldolase
Schmidtea mediterranea mk4.035731.04   Probable transaldolase
Schmidtea mediterranea mk4.035731.03  
Trypanosoma brucei gambiense Tbg972.8.5600   transaldolase, putative
Trypanosoma brucei Tb927.8.5600   transaldolase, putative
Trypanosoma congolense TcIL3000_0_10000   transaldolase, putative
Trypanosoma cruzi TcCLB.503477.20   transaldolase, putative
Trypanosoma cruzi TcCLB.507889.10   transaldolase, putative
Toxoplasma gondii TGME49_229360   transaldolase
Trichomonas vaginalis TVAG_272910   transaldolase total2, putative
Wolbachia endosymbiont of Brugia malayi Wbm0686   translaldolase

Essentiality

TcCLB.503477.20 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.5600 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5600 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.5600 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.5600 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0008 Escherichia coli non-essential goodall
b0825 Escherichia coli non-essential goodall
b2464 Escherichia coli non-essential goodall
b3946 Escherichia coli non-essential goodall
TGME49_229360 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Transaldolase (2.2.1.2 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.503477.20 (Trypanosoma cruzi), transaldolase, putative
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