Detailed view for PF3D7_0322000

Basic information

TDR Targets ID: 3717
Plasmodium falciparum, peptidyl-prolyl cis-trans isomerase

Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.0416 | Length (AA): 171 | MW (Da): 18953 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00160   Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD

Gene Ontology

Mouse over links to read term descriptions.
GO:0016018   cyclosporin A binding  
GO:0005829   cytosol  
GO:0000413   protein peptidyl-prolyl isomerization  
GO:0003755   peptidyl-prolyl cis-trans isomerase activity  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 171 1qng (A) 2 171 99.99 0 1 2.21 -1.75
2 171 2hq6 (A) 10 167 42.00 0 1 1.4 -0.84
2 171 1qng (A) 2 171 99.99 0 1 2.28995 -2.03

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

  • 1QNG:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 1QNH:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, merozoite, sporozoite, early ring, early schizont, early trophozoite, late ring, late schizont, late trophozoite, Oocyst, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile gametocyte, Female Gametocyte, Male Gametocyte. PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Sporozoite. Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_126792)

Species Accession Gene Product
Arabidopsis thaliana AT3G55920   Cyclophilin-like peptidyl-prolyl cis-trans isomerase family protein
Arabidopsis thaliana AT2G21130   peptidyl-prolyl cis-trans isomerase CYP19-2
Arabidopsis thaliana AT4G34870   rotamase cyclophilin 5
Arabidopsis thaliana AT4G38740   peptidyl-prolyl cis-trans isomerase CYP1
Babesia bovis BBOV_IV005000   cyclophilin, putative
Brugia malayi Bm1_46745   cyclophilin-type peptidyl-prolyl cis-trans isomerase-3, Bmcyp-3
Brugia malayi Bm1_55850   cyclophilin-type peptidyl-prolyl cis-trans isomerase-2, Bmcyp-2
Candida albicans CaO19.13826   Cyclophilin type peptidyl-prolyl cis-trans isomerase similar to S. cerevisiae CPR1 (YDR155C)
Candida albicans CaO19_6472   hypothetical protein
Candida albicans CaO19.6472   Cyclophilin type peptidyl-prolyl cis-trans isomerase similar to S. cerevisiae CPR1 (YDR155C)
Caenorhabditis elegans CELE_Y75B12B.2   Protein CYN-7
Caenorhabditis elegans CELE_Y75B12B.5   Protein CYN-3
Caenorhabditis elegans CELE_Y49A3A.5   Protein CYN-1
Caenorhabditis elegans CELE_ZK520.5   Protein CYN-2
Cryptosporidium hominis Chro.20441   20k cyclophilin
Cryptosporidium parvum cgd2_4120   20k cyclophilin, putative
Dictyostelium discoideum DDB_G0282359   cyclophilin-type peptidylprolyl cis-trans isomerase
Drosophila melanogaster Dmel_CG7768   CG7768 gene product from transcript CG7768-RA
Drosophila melanogaster Dmel_CG9916   Cyclophilin 1
Echinococcus granulosus EgrG_000920600   expressed protein
Echinococcus multilocularis EmuJ_000920600   expressed protein
Homo sapiens 101060363   peptidyl-prolyl cis-trans isomerase A-like
Homo sapiens ENSG00000108179   peptidylprolyl isomerase F
Homo sapiens ENSG00000196262   peptidylprolyl isomerase A (cyclophilin A)
Homo sapiens 650157   peptidyl-prolyl cis-trans isomerase A-like
Homo sapiens 101928624   peptidyl-prolyl cis-trans isomerase A-like
Leishmania braziliensis LbrM.33.1900   cyclophilin 4, putative
Leishmania braziliensis LbrM.25.0790   cyclophilin a
Leishmania braziliensis LbrM.23.0060   cyclophilin 11, putative
Leishmania donovani LdBPK_331730.1   cyclophilin, putative
Leishmania donovani LdBPK_230060.1   cyclophilin, putative
Leishmania donovani LdBPK_250940.1   cyclophilin a, putative
Leishmania infantum LinJ.23.0060   cyclophilin 11, putative
Leishmania infantum LinJ.33.1730   cyclophilin 4, putative
Leishmania infantum LinJ.25.0940   cyclophilin a
Leishmania major LmjF.33.1630   cyclophilin 4, putative
Leishmania major LmjF.23.0050   cyclophilin 11, putative
Leishmania major LmjF.25.0910   cyclophilin a
Leishmania mexicana LmxM.32.1630   cyclophilin, putative
Leishmania mexicana LmxM.25.0910   cyclophilin a
Leishmania mexicana LmxM.23.0050   cyclophilin, putative,peptidyl-prolyl cis-trans isomerase, putative
Loa Loa (eye worm) LOAG_06740   cyclophilin-type peptidyl-prolyl cis-trans isomerase-2
Loa Loa (eye worm) LOAG_03625   cyclophilin Ovcyp-2
Mus musculus 268373   peptidylprolyl isomerase A
Mus musculus ENSMUSG00000021868   peptidylprolyl isomerase F (cyclophilin F)
Neospora caninum NCLIV_031040   Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8), related
Neospora caninum NCLIV_019970   Peptidyl-prolyl cis-trans isomerase A, related
Oryza sativa 4347920   Os09g0571400
Oryza sativa 4328121   Os02g0121300
Onchocerca volvulus OVOC9856  
Onchocerca volvulus OVOC11375  
Plasmodium berghei PBANKA_1216500   peptidyl-prolyl cis-trans isomerase, putative
Plasmodium falciparum PF3D7_0322000   peptidyl-prolyl cis-trans isomerase
Plasmodium knowlesi PKNH_0818800   peptidyl-prolyl cis-trans isomerase, putative
Plasmodium vivax PVX_095135   cyclophilin, putative
Plasmodium vivax PVX_220290   unspecified product
Plasmodium yoelii PY03668   peptidyl-prolyl cis-trans isomerase, cyclophilin-type
Saccharomyces cerevisiae YDR155C   peptidylprolyl isomerase CPR1
Schistosoma japonicum Sjp_0001030   ko:K01802 peptidylprolyl isomerase [EC5.2.1.8], putative
Schistosoma mansoni Smp_040130   cyclophilin
Schmidtea mediterranea mk4.002269.05   Peptidyl-prolyl cis-trans isomerase
Schmidtea mediterranea mk4.002976.00   Peptidyl-prolyl cis-trans isomerase
Schmidtea mediterranea mk4.002269.03   Peptidyl-prolyl cis-trans isomerase
Schmidtea mediterranea mk4.004974.02   Peptidyl-prolyl cis-trans isomerase
Schmidtea mediterranea mk4.006784.02   Peptidyl-prolyl cis-trans isomerase
Schmidtea mediterranea mk4.008473.01   Peptidyl-prolyl cis-trans isomerase
Schmidtea mediterranea mk4.002499.01   Peptidyl-prolyl cis-trans isomerase
Trypanosoma brucei gambiense Tbg972.11.920   cyclophilin a,cyclophilin type peptidyl-prolyl cis-trans isomerase
Trypanosoma brucei gambiense Tbg.972.2.250   cyclophilin-type peptidyl-prolyl cis-trans isomerase, putative,cyclophilin, putative
Trypanosoma brucei gambiense Tbg.972.2.170   cyclophilin-type peptidyl-prolyl cis-trans isomerase, putative,cyclophilin, putative
Trypanosoma brucei gambiense Tbg972.8.1650   cyclophilin type peptidyl-prolyl cis-trans isomerase, putative,peptidyl-prolyl cis-trans isomerase, putative
Trypanosoma brucei Tb927.11.880   cyclophilin a
Trypanosoma brucei Tb927.8.2000   cyclophilin, putative
Trypanosoma brucei Tb927.2.1560   peptidyl-prolyl cis-trans isomerase, putative
Trypanosoma brucei Tb927.2.1680   peptidyl-prolyl cis-trans isomerase, putative
Trypanosoma congolense TcIL3000_0_06330   cyclophilin a, putative
Trypanosoma congolense TcIL3000_8_2030   peptidyl-prolyl cis-trans isomerase, putative
Trypanosoma congolense TcIL3000_0_06320   cyclophilin a, putative
Trypanosoma congolense TcIL3000.11.810   cyclophilin a, putative
Trypanosoma cruzi TcCLB.509499.10   cyclophilin, putative
Trypanosoma cruzi TcCLB.509215.40   peptidyl-prolyl cis-trans isomerase, putative
Trypanosoma cruzi TcCLB.510259.50   21 kDa cyclophilin, putative
Trypanosoma cruzi TcCLB.506925.300   cyclophilin type peptidyl-prolyl cis-trans isomerase
Trypanosoma cruzi TcCLB.510947.50   rotamase, putative
Toxoplasma gondii TGME49_230520   cyclophilin 1, putative
Toxoplasma gondii TGME49_205700   cyclophilin precursor
Theileria parva TP01_0244   cyclophilin 1
Trichomonas vaginalis TVAG_137880   peptidyl-prolyl cis-trans isomerase A, ppia, putative

Essentiality

PF3D7_0322000 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.03.0250 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.03.0250 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.03.0250 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.03.0250 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.2000 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.2000 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.2000 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.2000 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y75B12B.2 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_1216500 Plasmodium berghei Slow plasmo
TGME49_205700 Toxoplasma gondii Probably non-essential sidik
TGME49_230520 Toxoplasma gondii Probably non-essential sidik
TGME49_205700 Toxoplasma gondii Essentiality uncertain sidik
TGME49_230520 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) multi-cellular organism (CARO:0000012) bloodstream stage (PLO:0040) in vivo inhibition (TDR:00016) Plasmodium falciparum 375742   378360   406919   572633   572634   572635  
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 9716503
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Plasmodium falciparum 375742   378360   406919   572633   572634   572635  
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 9716503

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6


Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens peptidylprolyl isomerase F Compounds References
Rattus norvegicus Cyclophilin A Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0172 0.407 0.8888
0.0166 0.4174 1
0.0047 1 1
0.016363 0.252693 0.252693
0.0071 0.297 1
0.0046 1 0.5
0.0097 0.2512 0.247

Assayability

Assay information

  • Assay for FK-Binding Protein Peptidyl-Prolyl Isomerase Activity (5.2.1.8 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Plasmodium falciparum ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    PF3D7_0322000 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Plasmodium falciparum ( 3 )

Bibliographic References

13 literature references were collected for this gene.

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Gene identifier PF3D7_0322000 (Plasmodium falciparum), peptidyl-prolyl cis-trans isomerase
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