Detailed view for TcCLB.511809.70

Basic information

TDR Targets ID: 37617
Trypanosoma cruzi, DNA primase small subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.066 | Length (AA): 412 | MW (Da): 47187 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01896   DNA primase small subunit

Gene Ontology

Mouse over links to read term descriptions.
GO:0006269   DNA replication, synthesis of RNA primer  
GO:0003896   DNA primase activity  

Metabolic Pathways

Purine metabolism (KEGG)
Pyrimidine metabolism (KEGG)
Global map (KEGG)
DNA replication (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 400 1g71 (A) 6 344 20.00 0 1 0.89 -0.13
46 401 1v33 (A) 15 345 21.00 0 0.9 1.07 -0.39
3 412 4lim (A) 11 390 29.00 0 1 1.22835 -0.11
4 412 4lik (A) 10 406 35.00 0 1 1.27392 -0.09
59 375 4lik (A) 35 346 40.00 0 1 1.18712 -0.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128062)

Species Accession Gene Product
Arabidopsis thaliana AT5G41880   DNA primase POLA3
Babesia bovis BBOV_II005330   DNA primase small subunit, putative
Brugia malayi Bm1_44765   DNA primase, eukaryotic-type, small subunit family protein
Candida albicans CaO19.11513   DNA primase small subunit
Candida albicans CaO19.4030   DNA primase small subunit
Caenorhabditis elegans CELE_T24C4.5   Protein T24C4.5
Caenorhabditis elegans CELE_F58A4.4   Protein PRI-1, isoform B
Cryptosporidium hominis Chro.40171   DNA primase small subunit
Cryptosporidium hominis Chro.40170   small subunit DNA primase
Cryptosporidium parvum cgd4_1490   DNA primase small subunit
Dictyostelium discoideum DDB_G0285853   DNA polymerase alpha primase subunit
Drosophila melanogaster Dmel_CG7108   DNA polymerase alpha 50kD
Echinococcus granulosus EgrG_001186000   DNA primase small subunit
Entamoeba histolytica EHI_129630   DNA primase small subunit, putative
Entamoeba histolytica EHI_010140   DNA primase small subunit, putative
Entamoeba histolytica EHI_103530   DNA primase small subunit, putative
Echinococcus multilocularis EmuJ_001186000   DNA primase small subunit
Giardia lamblia GL50803_114670   DNA pol/primase, small sub
Homo sapiens ENSG00000198056   primase, DNA, polypeptide 1 (49kDa)
Leishmania braziliensis LbrM.22.0370   DNA primase small subunit, putative
Leishmania donovani LdBPK_220270.1   DNA primase small subunit, putative
Leishmania infantum LinJ.22.0270   DNA primase small subunit, putative
Leishmania major LmjF.22.0390   DNA primase small subunit, putative
Leishmania mexicana LmxM.22.0390   DNA primase small subunit, putative
Loa Loa (eye worm) LOAG_03041   DNA primase
Mus musculus ENSMUSG00000025395   DNA primase, p49 subunit
Neospora caninum NCLIV_026380   DNA primase small subunit, putative
Oryza sativa 9267485   Os05g0358200
Onchocerca volvulus OVOC8556   DNA primase small subunit homolog
Plasmodium berghei PBANKA_1302600   DNA primase small subunit, putative
Plasmodium falciparum PF3D7_1438700   DNA primase small subunit
Plasmodium knowlesi PKNH_1243700   DNA primase small subunit, putative
Plasmodium yoelii PY00667   DNA primase small subunit
Saccharomyces cerevisiae YIR008C   DNA primase subunit PRI1
Schistosoma japonicum Sjp_0099880   DNA primase small subunit, putative
Schistosoma mansoni Smp_160190   hypothetical protein
Schmidtea mediterranea mk4.015509.00   DNA primase small subunit
Schmidtea mediterranea mk4.001348.02   DNA primase small subunit
Trypanosoma brucei gambiense Tbg972.7.2570   DNA primase small subunit, putative
Trypanosoma brucei Tb927.7.2310   DNA primase small subunit, putative
Trypanosoma congolense TcIL3000_7_1640   DNA primase small subunit, putative
Trypanosoma cruzi TcCLB.511809.70   DNA primase small subunit, putative
Toxoplasma gondii TGME49_260680   small subunit DNA primase, putative
Theileria parva TP02_0837   DNA primase small subunit, putative
Trichomonas vaginalis TVAG_099330   DNA primase, putative

Essentiality

TcCLB.511809.70 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.2310 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.2310 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.2310 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.2310 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F58A4.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F58A4.4 Caenorhabditis elegans larval arrest wormbase
CELE_F58A4.4 Caenorhabditis elegans larval lethal wormbase
CELE_F58A4.4 Caenorhabditis elegans sterile wormbase
CELE_T24C4.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_T24C4.5 Caenorhabditis elegans sterile wormbase
YIR008C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1302600 Plasmodium berghei Essential plasmo
TGME49_260680 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

156 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.511809.70 (Trypanosoma cruzi), DNA primase small subunit, putative
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