Detailed view for TcCLB.510243.100

Basic information

TDR Targets ID: 37815
Trypanosoma cruzi, chloride channel protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.2159 | Length (AA): 967 | MW (Da): 107357 | Paralog Number: 4

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 12

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00571   CBS domain
PF00654   Voltage gated chloride channel

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0005247   voltage-gated chloride channel activity  
GO:0005216   ion channel activity  
GO:0055085   transmembrane transport  
GO:0006821   chloride transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 12 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
110 629 1ots (A) 20 450 21.00 0 1 0.68 -1.28
126 634 1kpl (A) 37 457 22.00 0 0.91 0.71 -1.45
780 895 1y5h (A) 3 123 15.00 0 0.4 0.32 -0.97
111 451 1kpl (B) 22 331 21.00 0.0000094 0.75 0.390937 1.31
122 654 3org (A) 88 544 30.00 0 1 0.587489 0.76
837 892 2j9l (A) 674 729 34.00 0.0023 0.84 0.376211 0.98
844 951 2o16 (A) 25 144 16.00 0.008 0.18 0.233986 -0.2
844 956 5awe (A) 5 129 19.00 0.00021 0.14 0.259156 -0.14
845 892 3gby (A) 70 118 17.00 0 0.39 0.116038 1.09
845 892 2ef7 (A) 72 120 27.00 0 0.98 0.296038 0.88
847 900 2yzi (A) 80 132 25.00 0 0.83 0.266243 0.91
850 965 1zfj (A) 101 218 15.00 0.011 0.17 0.220259 0.01

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_128607)

Species Accession Gene Product
Arabidopsis thaliana AT5G26240   chloride channel protein CLC-d
Arabidopsis thaliana AT5G40890   chloride channel protein CLC-a
Arabidopsis thaliana AT5G33280   putative chloride channel-like protein CLC-g
Arabidopsis thaliana AT5G49890   chloride channel protein CLC-c
Arabidopsis thaliana AT3G27170   chloride channel protein CLC-b
Brugia malayi Bm1_09345   chloride channel protein 7
Caenorhabditis elegans CELE_R07B7.1   Protein CLH-6
Dictyostelium discoideum DDB_G0278639   CLC 6/7 family protein
Dictyostelium discoideum DDB_G0276229   CLC 6/7 family protein
Dictyostelium discoideum DDB_G0294096   CLC 6/7 family protein
Dictyostelium discoideum DDB_G0276865   CLC 6/7 family protein
Drosophila melanogaster Dmel_CG8594   Chloride channel-b
Echinococcus granulosus EgrG_000706100   H:Cl exchange transporter 7
Echinococcus multilocularis EmuJ_000706100   H(+):Cl() exchange transporter 7
Homo sapiens ENSG00000103249   chloride channel, voltage-sensitive 7
Leishmania braziliensis LbrM.04.1010   chloride channel protein, putative
Leishmania braziliensis LbrM.32.3670   chloride channel protein, putative
Leishmania donovani LdBPK_323570.1   chloride channel protein, putative
Leishmania donovani LdBPK_041010.1   chloride channel protein, putative
Leishmania infantum LinJ.04.1010   chloride channel protein, putative
Leishmania infantum LinJ.32.3570   chloride channel protein, putative
Leishmania major LmjF.32.3370   chloride channel protein, putative
Leishmania major LmjF.04.1000   chloride channel protein, putative
Leishmania mexicana LmxM.04.1000   chloride channel protein, putative
Leishmania mexicana LmxM.31.3370   chloride channel protein, putative
Loa Loa (eye worm) LOAG_09330   chloride channel protein 7
Loa Loa (eye worm) LOAG_05653   hypothetical protein
Mus musculus ENSMUSG00000036636   chloride channel 7
Oryza sativa 4329678   Os02g0558100
Oryza sativa 9267520   Os01g0876100
Oryza sativa 4352132   Os12g0438600
Oryza sativa 4337190   Os04g0644800
Onchocerca volvulus OVOC2472  
Schistosoma japonicum Sjp_0039340   ko:K05016 chloride channel 7, putative
Schistosoma mansoni Smp_058360.1   chloride channel protein
Schistosoma mansoni Smp_058360.2   chloride channel protein
Schmidtea mediterranea mk4.005367.01   H()/Cl(-) exchange transporter 7
Schmidtea mediterranea mk4.005367.02   H()/Cl(-) exchange transporter 7
Schmidtea mediterranea mk4.005367.03  
Trypanosoma brucei gambiense Tbg972.9.4670   chloride channel protein, putative
Trypanosoma brucei gambiense Tbg972.11.18820   chloride channel protein, putative
Trypanosoma brucei Tb927.11.16690   Voltage-gated Chloride channel Like 3
Trypanosoma brucei Tb927.9.8540   Voltage-gated Chloride channel Like 1
Trypanosoma congolense TcIL3000_9_2970   chloride channel protein, putative
Trypanosoma congolense TcIL3000.11.16580   chloride channel protein, putative
Trypanosoma cruzi TcCLB.510243.100   chloride channel protein, putative
Trypanosoma cruzi TcCLB.510575.130   chloride channel protein, putative
Trypanosoma cruzi TcCLB.507089.200   chloride channel protein, putative
Trypanosoma cruzi TcCLB.503839.5   chloride channel protein, putative
Trypanosoma cruzi TcCLB.511027.10   chloride channel protein, putative

Essentiality

TcCLB.510243.100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.0430 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.0430 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.0430 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.0430 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.01.8420 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.8420 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.8420 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.8420 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0008 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.510243.100 (Trypanosoma cruzi), chloride channel protein, putative
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