Detailed view for cgd6_20

Basic information

TDR Targets ID: 379467
Cryptosporidium parvum, inosine-5-monophosphate dehydrogenase
Source Database / ID: 

pI: 5.5537 | Length (AA): 402 | MW (Da): 43283 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00478   IMP dehydrogenase / GMP reductase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0016491   oxidoreductase activity  
GO:0003938   IMP dehydrogenase activity  
GO:0055114   oxidation reduction  
GO:0006164   purine nucleotide biosynthetic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 394 4ixh (A) 1 392 99.00 0 1 1.95712 -0.58
143 214 3gnn (A) 210 277 19.00 0 0.97 0.500004 -1.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3FFS:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3KHJ:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4IXH:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126887)

Species Accession Gene Product
Arabidopsis thaliana AT1G16350   inosine-5'-monophosphate dehydrogenase 2
Arabidopsis thaliana AT1G79470   inosine-5'-monophosphate dehydrogenase
Babesia bovis BBOV_IV006900   inosine-5'-monophosphate dehydrogenase, putative
Brugia malayi Bm1_31240   inosine-5'-monophosphate dehydrogenase family protein
Candida albicans CaO19.7689   guanosine nucleotide biosynthesis
Candida albicans CaO19.18   guanosine nucleotide biosynthesis
Candida albicans CaO19.19   N terminus of Inosine-5'-monophosphate dehydrogenase
Candida albicans CaO19.7690   N terminus of Inosine-5'-monophosphate dehydrogenase
Caenorhabditis elegans CELE_T22D1.3   Protein T22D1.3, isoform B
Cryptosporidium hominis Chro.60012   inosine-5-monophosphate dehydrogenase
Cryptosporidium parvum cgd6_20   inosine-5-monophosphate dehydrogenase
Dictyostelium discoideum DDB_G0283701   IMP dehydrogenase
Drosophila melanogaster Dmel_CG1799   raspberry
Escherichia coli b2508   IMP dehydrogenase
Echinococcus granulosus EgrG_000120200   inosine 5' monophosphate dehydrogenase 2
Echinococcus multilocularis EmuJ_000120200   inosine 5' monophosphate dehydrogenase 2
Homo sapiens ENSG00000106348   IMP (inosine 5'-monophosphate) dehydrogenase 1
Homo sapiens ENSG00000178035   IMP (inosine 5'-monophosphate) dehydrogenase 2
Leishmania braziliensis LbrM.19.1820   inosine-5-monophosphate dehydrogenase
Leishmania braziliensis LbrM.17.0790   guanosine monophosphate reductase, putative
Leishmania donovani LdBPK_191590.1   inosine-5'-monophosphate dehydrogenase
Leishmania donovani LdBPK_170870.1   GMP reductase
Leishmania infantum LinJ.19.1590   inosine-5-monophosphate dehydrogenase
Leishmania infantum LinJ.17.0870   guanosine monophosphate reductase, putative
Leishmania major LmjF.17.0725   guanosine monophosphate reductase
Leishmania major LmjF.19.1560   inosine-5-monophosphate dehydrogenase
Leishmania mexicana LmxM.17.0725   guanosine monophosphate reductase
Leishmania mexicana LmxM.19.1560   inosine-5-monophosphate dehydrogenase
Loa Loa (eye worm) LOAG_00767   IMP dehydrogenase 1
Mycobacterium leprae ML0388   Probable inosine-5'-monophosphate dehydrogenase GuaB3 (IMP dehydrogenase 2) (inosinic acid dehydrogenase) (inosinate dehydrogena
Mycobacterium leprae ML0387   Probable inosine-5'-monophosphate dehydrogenase GuaB2 (IMP dehydrogenase) (IMPDH) (IMPD)
Mus musculus ENSMUSG00000062867   inosine 5'-phosphate dehydrogenase 2
Mus musculus 100042069   predicted gene 15210
Mus musculus ENSMUSG00000003500   inosine 5'-phosphate dehydrogenase 1
Mycobacterium tuberculosis Rv3411c   Probable inosine-5'-monophosphate dehydrogenase GuaB2 (imp dehydrogenase) (inosinic acid dehydrogenase) (inosinate dehydrogenase
Mycobacterium tuberculosis Rv3410c   Probable inosine-5'-monophosphate dehydrogenase GuaB3 (imp dehydrogenase) (inosinic acid dehydrogenase) (inosinate dehydrogenase
Mycobacterium ulcerans MUL_0901   inosine 5-monophosphate dehydrogenase
Mycobacterium ulcerans MUL_0900   inosine 5'-monophosphate dehydrogenase
Neospora caninum NCLIV_032920   hypothetical protein
Oryza sativa 4334308   Os03g0780500
Plasmodium berghei PBANKA_0821700   inosine-5'-monophosphate dehydrogenase, putative
Plasmodium falciparum PF3D7_0920800   inosine-5'-monophosphate dehydrogenase
Plasmodium knowlesi PKNH_0718800   inosine-5'-monophosphate dehydrogenase, putative
Plasmodium vivax PVX_099455   inosine-5'-monophosphate dehydrogenase, putative
Plasmodium yoelii PY03601   inosine-5'-monophosphate dehydrogenase
Saccharomyces cerevisiae YHR216W   IMP dehydrogenase IMD2
Saccharomyces cerevisiae YML056C   IMP dehydrogenase IMD4
Saccharomyces cerevisiae YLR432W   IMP dehydrogenase IMD3
Schistosoma japonicum Sjp_0061360   ko:K00088 IMP dehydrogenase [EC1.1.1.205], putative
Schistosoma mansoni Smp_012930   inosine-5-monophosphate dehydrogenase
Schmidtea mediterranea mk4.000519.05   Inosine-5'-monophosphate dehydrogenase
Schmidtea mediterranea mk4.000519.04  
Schmidtea mediterranea mk4.037673.02   Inosine-5'-monophosphate dehydrogenase
Trypanosoma brucei gambiense Tbg972.10.19740   inosine-5-monophosphate dehydrogenase,IMP dehydrogenase, putative
Trypanosoma brucei gambiense Tbg972.5.2930   inosine-5-monophosphate dehydrogenase, putative
Trypanosoma brucei Tb927.10.16120   inosine-5'-monophosphate dehydrogenase
Trypanosoma brucei Tb927.5.2080   GMP reductase
Trypanosoma congolense TcIL3000_5_1940   GMP reductase
Trypanosoma cruzi TcCLB.508909.20   GMP reductase
Trypanosoma cruzi TcCLB.506519.130   GMP reductase
Trypanosoma cruzi TcCLB.507211.40   inosine-5'-monophosphate dehydrogenase, putative
Trypanosoma cruzi TcCLB.511351.9   inosine-5'-monophosphate dehydrogenase, putative
Trypanosoma cruzi TcCLB.511301.110   inosine-5'-monophosphate dehydrogenase, putative
Toxoplasma gondii TGME49_233110   IMP dehydrogenas
Theileria parva TP03_0220   Inosine-5'-monophosphate dehydrogenase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0527   IMP dehydrogenase

Essentiality

cgd6_20 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu3472 Mycobacterium tuberculosis essential nmpdr
Tb927.5.2080 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.2080 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.2080 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.2080 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.16120 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.16120 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.16120 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.16120 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b2508 Escherichia coli essential goodall
PBANKA_0821700 Plasmodium berghei Essential plasmo
TGME49_233110 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

231 chemical compounds are associated with this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens IMP (inosine 5'-monophosphate) dehydrogenase 2 Compounds References
Bacillus anthracis Inosine-5'-monophosphate dehydrogenase Compounds References
Homo sapiens IMP (inosine 5'-monophosphate) dehydrogenase 1 Compounds References
Plasmodium falciparum inosine-5'-monophosphate dehydrogenase Compounds References
Escherichia coli IMP dehydrogenase Compounds References
Mus musculus inosine 5'-phosphate dehydrogenase 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • ChEMBL
  • Inhibition of Cryptosporidium parvum recombinant IMPDH expressed in Escherichia coli TX685 assessed as NADH production by fluorescence assay in absence of 0.05% fatty acid free bovine serum albumin
  • ChEMBL
  • Inhibition of Cryptosporidium parvum recombinant IMPDH expressed in Escherichia coli TX685 assessed as NADH production by fluorescence assay in presence of 0.05% fatty acid free bovine serum albumin
  • ChEMBL
  • Ratio of IC50 for Cryptosporidium parvum recombinant IMPDH in presence of 0.05% fatty acid free bovine serum albumin to IC50 for Cryptosporidium parvum recombinant IMPDH in absence of 0.05% fatty acid free bovine serum albumin
  • ChEMBL
  • Ratio of campaign IC50 to compound IC50 for Cryptosporidium parvum recombinant IMPDH expressed in Escherichia coli TX685 in absence of 0.05% fatty acid free bovine serum albumin
  • ChEMBL
  • Inhibition of Cryptosporidium parvum recombinant IMPDH expressed in Escherichia coli guaB assessed as NADH production by fluorescence assay
  • ChEMBL
  • Inhibition of recombinant Cryptosporidium parvum IMPDH expressed in Escherichia coli assessed as NADH production preincubated for 5 mins by fluorescence assay in presence of bovine serum albumin
  • ChEMBL
  • Inhibition of recombinant Cryptosporidium parvum IMPDH expressed in Escherichia coli assessed as NADH production preincubated for 5 mins by fluorescence assay
  • ChEMBL
  • Inhibition of Cryptosporidium parvum recombinant IMPDH expressed in Escherichia coli assessed as production of NADH in presence of 0.05% fatty acid free BSA incubated for 5 mins by fluorescence based assay
  • ChEMBL
  • Inhibition of Cryptosporidium parvum recombinant IMPDH expressed in Escherichia coli assessed as production of NADH incubated for 5 mins by fluorescence based assay
  • ChEMBL
  • Mixed type inhibition of recombinant Cryptosporidium parvum IMPDH with respect to NAD+
  • ChEMBL
  • Inhibition of recombinant Cryptosporidium parvum IMPDH assessed as production of NADH by fluorescence assay in presence of 0.05% BSA
  • ChEMBL
  • Inhibition of recombinant Cryptosporidium parvum IMPDH assessed as production of NADH by fluorescence assay
  • ChEMBL
  • Competitive inhibition of Cryptosporidium parvum N-terminal His6-tagged IMPDH-mediated NADH production expressed in Escherichia coli BL21(DE3) using NAD as substrate by Lineweaver-Burk plot analysis
  • ChEMBL
  • Noncompetitive inhibition of Cryptosporidium parvum N-terminal His6-tagged IMPDH-mediated NADH production expressed in Escherichia coli BL21(DE3) using IMP as substrate by Lineweaver-Burk plot analysis
  • ChEMBL
  • Inhibition of Cryptosporidium parvum N-terminal His6-tagged IMPDH-mediated NADH production expressed in Escherichia coli BL21(DE3) preincubated for 5 mins prior to substrate addition by fluorescence assay in presence of BSA
  • ChEMBL
  • Inhibition of Cryptosporidium parvum N-terminal His6-tagged IMPDH-mediated NADH production expressed in Escherichia coli BL21(DE3) preincubated for 5 mins prior to substrate addition by fluorescence assay
  • ChEMBL
  • Inhibition of Cryptosporidium IMPDH preincubated for 10 mins before substrate addition by spectrophotometry
  • ChEMBL
  • Un-competitive inhibition of Cryptosporidium IMPDH preincubated for 10 mins before IMP substrate addition by spectrophotometry
  • ChEMBL
  • Competitive inhibition of Cryptosporidium IMPDH preincubated for 10 mins before NAD+ substrate addition by spectrophotometry
  • ChEMBL
  • Non-competitive inhibition of Cryptosporidium IMPDH preincubated for 10 mins before NAD+ substrate addition by spectrophotometry
  • ChEMBL
  • Inhibition of Cryptosporidium parvum IMPDH assessed as reduction in NADH production

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier cgd6_20 (Cryptosporidium parvum), inosine-5-monophosphate dehydrogenase
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