Detailed view for TcCLB.509649.30

Basic information

TDR Targets ID: 38251
Trypanosoma cruzi, 19S proteasome non-atpase subunit 8
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.4579 | Length (AA): 406 | MW (Da): 45608 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01398   JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PF13012   Maintenance of mitochondrial structure and function

Gene Ontology

Mouse over links to read term descriptions.
GO:0005838   proteasome regulatory particle  
GO:0005515   protein binding  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
78 266 2o95 (A) 1 183 43.00 0 1 0.989317 -0.61
81 369 4d10 (F) 36 316 21.00 0 1 0.919623 0.35
82 261 5w83 (A) 4 177 40.00 0 1 0.92965 -0.69
246 406 4uxv (A) 236 416 28.00 0 0.3 0.477852 0.54
313 401 5jsb (B) 18 106 19.00 0.14 0.05 0.486012 -0.79

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127933)

Species Accession Gene Product
Arabidopsis thaliana AT3G11270   protein MATERNAL EFFECT EMBRYO ARREST 34
Arabidopsis thaliana AT5G05780   probable 26S proteasome non-ATPase regulatory subunit 8A
Babesia bovis BBOV_II003480   26S proteasome regulatory particle non-ATPase subunit8, putative
Brugia malayi Bm1_36060   26S proteasome non-ATPase regulatory subunit 7, putative
Candida albicans CaO19.3168   similar to S. cerevisiae RPN8 (YOR261C) subunit of the 19S regulatory particle of the 26S proteasome
Candida albicans CaO19.10677   similar to S. cerevisiae RPN8 (YOR261C) subunit of the 19S regulatory particle of the 26S proteasome
Caenorhabditis elegans CELE_R12E2.3   Protein RPN-8
Cryptosporidium hominis Chro.70327   26S proteasome regulatory particle non-ATPase subunit8
Cryptosporidium parvum cgd7_2900   26S proteasome regulatory subunit, inactive JAB domain protein
Dictyostelium discoideum DDB_G0279633   26S proteasome non-ATPase regulatory subunit 7
Drosophila melanogaster Dmel_CG3416   Regulatory particle non-ATPase 8
Echinococcus granulosus EgrG_000687300   26S proteasome non ATPase regulatory subunit 7
Entamoeba histolytica EHI_030170   26S proteasome non-ATPase regulatory subunit, putative
Echinococcus multilocularis EmuJ_000687300   26S proteasome non ATPase regulatory subunit 7
Giardia lamblia GL50803_7896   26S proteasome non-ATPase regulatory subunit 7
Homo sapiens ENSG00000103035   proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
Leishmania braziliensis LbrM.32.0460   proteasome regulatory non-ATP-ase subunit 8, putative,26S proteasome regulatory subunit, putative
Leishmania donovani LdBPK_320400.1   19S proteasome non-atpase subunit 8
Leishmania infantum LinJ.32.0400   proteasome regulatory non-ATP-ase subunit 8, putative,26S proteasome regulatory subunit, putative
Leishmania major LmjF.32.0390   proteasome regulatory non-ATP-ase subunit 8, putative,26S proteasome regulatory subunit, putative
Leishmania mexicana LmxM.31.0390   proteasome regulatory non-ATP-ase subunit 8, putative,26S proteasome regulatory subunit, putative
Loa Loa (eye worm) LOAG_01378   26S proteasome non-ATPase regulatory subunit 7
Mus musculus ENSMUSG00000039067   proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
Neospora caninum NCLIV_037150   hypothetical protein
Oryza sativa 4337291   Os04g0661900
Plasmodium berghei PBANKA_0813900   26S proteasome regulatory subunit RPN8, putative
Plasmodium falciparum PF3D7_0912900   26S proteasome regulatory subunit RPN8, putative
Plasmodium knowlesi PKNH_0710800   26S proteasome regulatory subunit RPN8, putative
Plasmodium vivax PVX_099080   26S proteasome regulatory subunit RPN8, putative
Plasmodium yoelii PY05051   probable 26s proteasome regulatory subunit s12
Saccharomyces cerevisiae YOR261C   proteasome regulatory particle lid subunit RPN8
Schistosoma japonicum Sjp_0303130   ko:K03038 26S proteasome regulatory subunit N8, putative
Schistosoma mansoni Smp_026630   26S proteasome non-ATPase regulatory subunit 7
Schmidtea mediterranea mk4.004239.01   26S proteasome non-ATPase regulatory subunit 7
Schmidtea mediterranea mk4.021047.00   26S proteasome non-ATPase regulatory subunit 7
Schmidtea mediterranea mk4.026926.00   26S proteasome non-ATPase regulatory subunit 7
Schmidtea mediterranea mk4.009761.00   26S proteasome non-ATPase regulatory subunit 7
Trypanosoma brucei gambiense Tbg972.10.17670   proteasome regulatory non-ATPase subunit 8,26S proteasome regulatory subunit, putative
Trypanosoma brucei Tb927.10.14530   19S proteasome non-atpase subunit 8
Trypanosoma congolense TcIL3000_10_12430   19S proteasome non-atpase subunit 8
Trypanosoma cruzi TcCLB.509649.30   19S proteasome non-atpase subunit 8
Trypanosoma cruzi TcCLB.511285.90   19S proteasome non-atpase subunit 8
Toxoplasma gondii TGME49_269250   Mov34/MPN/PAD-1 family protein
Theileria parva TP04_0236   26S proteasome regulatory subunit, putative
Trichomonas vaginalis TVAG_124160   Clan MP, family M67, Poh1-like metallopeptidase
Trichomonas vaginalis TVAG_015750   Clan MP, family M67, Poh1-like metallopeptidase

Essentiality

TcCLB.509649.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.14530 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.14530 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.14530 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.14530 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_R12E2.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_R12E2.3 Caenorhabditis elegans larval arrest wormbase
CELE_R12E2.3 Caenorhabditis elegans larval lethal wormbase
CELE_R12E2.3 Caenorhabditis elegans slow growth wormbase
YOR261C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0813900 Plasmodium berghei Essential plasmo
TGME49_269250 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TcCLB.509649.30 (Trypanosoma cruzi), 19S proteasome non-atpase subunit 8
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