Detailed view for PF3D7_0516600

Basic information

TDR Targets ID: 4197
Plasmodium falciparum, sporozoite surface antigen MB2
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.5203 | Length (AA): 1610 | MW (Da): 187039 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00009   Elongation factor Tu GTP binding domain
PF11987   Translation-initiation factor 2

Gene Ontology

Mouse over links to read term descriptions.
GO:0020011   apicoplast  
GO:0005886   plasma membrane  
GO:0005634   nucleus  
GO:0005525   GTP binding  
GO:0003924   GTPase activity  
GO:0003723   RNA binding  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 20 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
979 1150 1fzq (A) 7 173 19.00 0.000005 0.93 0.4 -1.18
988 1361 1kk0 (A) 6 410 16.00 0 1 0.08 -0.04
104 1312 3ffz (A) 5 1209 21.00 0.0022 1 0.694032 1.48
890 1513 4kjz (D) 4 469 21.00 0 1 -0.0795224 1.77
942 1514 4kjz (A) 36 470 30.00 0 1 0.231001 1.18
987 1163 2lkc (A) 3 178 41.00 0 1 0.663038 -0.8
988 1605 1g7s (A) 1 546 20.00 0.000000000017 1 0.301951 0.95
990 1136 1ha3 (B) 9 174 32.00 0.014 1 0.477404 -0.95
990 1150 1d2e (A) 55 243 27.00 0.03 1 0.3451 -0.94
1380 1510 1z9b (A) 17 135 34.00 0.0000000039 0.76 0.268466 0.92
1518 1608 1d1n (A) 143 233 37.00 0 1 0.509622 -0.2
1520 1610 2crv (A) 7 101 31.00 0.000000017 1 0.432622 -0.43
908 1518 4kjz (D) 11 469 20.00 0 1 -0.169872 2.24
912 1296 4b3x (A) 1 363 26.00 0 1 0.29819 0.66
942 1519 4kjz (A) 14 470 28.00 0 1 0.239195 1.27
992 1168 2lkc (A) 3 178 41.00 0 1 0.667898 -0.8
995 1141 1ha3 (B) 9 174 32.00 0.013 1 0.476622 -0.95
1001 1612 1g7s (A) 9 548 19.00 0 1 -0.183253 1.78
1523 1613 1d1n (A) 143 233 37.00 0 1 0.514647 -0.2
1525 1615 2crv (A) 7 101 31.00 0.000000022 1 0.437647 -0.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile early schizont, late trophozoite. PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 32 hs, early trophozoite, Oocyst, Ring, Sporozoite, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte. Otto TD PlasmoDB
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_127126)

Species Accession Gene Product
Arabidopsis thaliana AT4G11160   translation initiation factor IF-2
Arabidopsis thaliana AT1G17220   translation initiation factor IF-2
Babesia bovis BBOV_II003130   elongation factor Tu GTP binding domain containing protein
Babesia bovis BBOV_I003420   elongation factor Tu GTP binding domain containing protein
Brugia malayi Bm1_34480   Elongation factor Tu GTP binding domain containing protein
Candida albicans CaO19.12634   Mitochondrial initiation factor 2
Candida albicans CaO19_5167   hypothetical protein
Candida albicans CaO19.5167   Mitochondrial initiation factor 2
Caenorhabditis elegans CELE_F46B6.6   Protein F46B6.6, isoform A
Chlamydia trachomatis CT_096   translation initiation factor IF-2
Dictyostelium discoideum DDB_G0275751   hypothetical protein
Drosophila melanogaster Dmel_CG12413   CG12413 gene product from transcript CG12413-RA
Escherichia coli b3168   translation initiation factor IF-2
Echinococcus granulosus EgrG_000227400   translation initiation factor IF 2
Echinococcus multilocularis EmuJ_000227400   translation initiation factor IF 2
Homo sapiens ENSG00000085760   mitochondrial translational initiation factor 2
Leishmania braziliensis LbrM.22.1120   translation initiation factor IF-2, putative
Leishmania donovani LdBPK_221060.1   translation initiation factor IF-2, putative
Leishmania infantum LinJ.22.1060   translation initiation factor IF-2, putative
Leishmania major LmjF.22.1240   translation initiation factor IF-2, putative
Leishmania mexicana LmxM.22.1240   translation initiation factor IF-2, putative
Loa Loa (eye worm) LOAG_01774   elongation factor Tu GTP binding domain-containing protein
Mycobacterium leprae ML1556c   PROBABLE TRANSLATION INITIATION FACTOR IF-2 INFB
Mus musculus ENSMUSG00000020459   mitochondrial translational initiation factor 2
Mycobacterium tuberculosis Rv2839c   Probable translation initiation factor if-2 InfB
Mycobacterium ulcerans MUL_2122   translation initiation factor IF-2
Neospora caninum NCLIV_067450   hypothetical protein
Oryza sativa 4339689   Os05g0575300
Oryza sativa 4347576   Os09g0515500
Plasmodium berghei PBANKA_0703600   translation initiation factor IF-2, putative
Plasmodium berghei PBANKA_1231400   sporozoite surface antigen MB2, putative
Plasmodium falciparum PF3D7_0516600   sporozoite surface antigen MB2
Plasmodium falciparum PF3D7_0827100   translation initiation factor IF-2, putative
Plasmodium knowlesi PKNH_1321200   translation initiation factor IF-2, putative
Plasmodium knowlesi PKNH_1016700   translation initiation factor IF-2, putative
Plasmodium vivax PVX_080420   MB2 protein, putative
Plasmodium vivax PVX_089010   translation initiation factor IF-2, putative
Plasmodium yoelii PY03311   Plasmodium falciparum MB2
Plasmodium yoelii PY06191   translation initiation factor IF-2
Saccharomyces cerevisiae YOL023W   translation initiation factor 2
Schistosoma japonicum Sjp_0211510   ko:K02519 translation initiation factor IF-2, putative
Schistosoma mansoni Smp_167220   mitochondrial translational initiation factor
Schmidtea mediterranea mk4.010079.01   Translation initiation factor IF-2, mitochondrial
Schmidtea mediterranea mk4.004315.04   Translation initiation factor IF-2, mitochondrial
Trypanosoma brucei gambiense Tbg972.7.3600   translation initiation factor IF-2, putative
Trypanosoma brucei Tb927.7.3280   translation initiation factor IF-2, putative
Trypanosoma congolense TcIL3000_7_2430   translation initiation factor IF-2, putative
Trypanosoma cruzi TcCLB.511821.110   translation initiation factor IF-2, putative
Trypanosoma cruzi TcCLB.510533.120   translation initiation factor IF-2, putative
Toxoplasma gondii TGME49_278550   elongation factor Tu GTP binding domain-containing protein
Treponema pallidum TP0891   translation initiation factor IF-2
Theileria parva TP04_0278   hypothetical protein
Theileria parva TP03_0192   translation initiation factor IF-2, putative
Theileria parva TP04_0279   hypothetical protein
Theileria parva TP04_0280   hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0537   translation initiation factor IF-2

Essentiality

PF3D7_0516600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.3280 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.3280 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.3280 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.3280 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3168 Escherichia coli essential goodall
CELE_F46B6.6 Caenorhabditis elegans slow growth wormbase
PBANKA_1231400 Plasmodium berghei Slow plasmo
TGME49_278550 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_0516600 (Plasmodium falciparum), sporozoite surface antigen MB2
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