Detailed view for TcCLB.511859.170

Basic information

TDR Targets ID: 42284
Trypanosoma cruzi, NADH-cytochrome b5 reductase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.1608 | Length (AA): 306 | MW (Da): 34632 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00175   Oxidoreductase NAD-binding domain
PF00970   Oxidoreductase FAD-binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0009055   electron carrier activity  
GO:0055114   oxidation reduction  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
27 306 5gv8 (A) 1001 1272 46.00 0 1 1.49043 -0.79
32 306 2eix (A) 43 281 47.00 0 1 1.37269 -0.62
34 306 1qx4 (A) 37 300 45.00 0 1 1.45296 -1.16
34 306 1umk (A) 37 300 47.00 0 1 1.49896 -1.17

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126955)

Species Accession Gene Product
Arabidopsis thaliana AT1G37130   nitrate reductase [NADH] 2
Arabidopsis thaliana AT1G77760   nitrate reductase [NADH] 1
Arabidopsis thaliana AT5G17770   NADH--cytochrome B5 reductase 1
Babesia bovis BBOV_II005800   hypothetical protein
Brugia malayi Bm1_53060   diaphorase
Candida albicans CaO19.9367   cytochrome b reductase
Candida albicans CaO19.1801   cytochrome b reductase
Caenorhabditis elegans CELE_T05H4.5   Protein HPO-19
Caenorhabditis elegans CELE_T05H4.4   Protein T05H4.4
Dictyostelium discoideum DDB_G0285399   NADH-cytochrome b5 reductase
Drosophila melanogaster Dmel_CG5946   CG5946 gene product from transcript CG5946-RB
Escherichia coli b1392   ring 1,2-phenylacetyl-CoA epoxidase, NAD(P)H oxidoreductase component
Echinococcus granulosus EgrG_000865900   NADH cytochrome b5 reductase 3
Echinococcus multilocularis EmuJ_000865900   NADH cytochrome b5 reductase 3
Homo sapiens ENSG00000100243   cytochrome b5 reductase 3
Homo sapiens ENSG00000159348   cytochrome b5 reductase 1
Homo sapiens ENSG00000166394   cytochrome b5 reductase 2
Leishmania braziliensis LbrM.13.0880   NADH-cytochrome b5 reductase, putative
Leishmania donovani LdBPK_130960.1   NADH-cytochrome b5 reductase, putative
Leishmania infantum LinJ.13.0960   NADH-cytochrome b5 reductase, putative
Leishmania major LmjF.13.1060   NADH-cytochrome b5 reductase, putative
Leishmania mexicana LmxM.13.1060   NADH-cytochrome b5 reductase, putative
Loa Loa (eye worm) LOAG_10211   diaphorase
Mus musculus ENSMUSG00000018042   cytochrome b5 reductase 3
Mus musculus ENSMUSG00000026456   cytochrome b5 reductase 1
Mus musculus ENSMUSG00000048065   cytochrome b5 reductase 2
Neospora caninum NCLIV_025010   hypothetical protein
Oryza sativa 4345798   Os08g0468700
Oryza sativa 4345795   Os08g0468100
Oryza sativa 4327480   Os01g0814900
Oryza sativa 4330867   Os02g0770800
Oryza sativa 4339153   Os05g0488900
Plasmodium berghei PBANKA_1143400   NADH-cytochrome b5 reductase, putative
Plasmodium falciparum PF3D7_1367500   NADH-cytochrome b5 reductase, putative
Plasmodium knowlesi PKNH_1103700   NADH-cytochrome b5 reductase, putative
Plasmodium vivax PVX_115335   oxidoreductase NAD-binding domain containing protein
Plasmodium yoelii PY03083   FAD/NAD-binding Cytochrome reductase, putative
Saccharomyces cerevisiae YIL043C   Cbr1p
Schistosoma japonicum Sjp_0303830   ko:K00326 cytochrome-b5 reductase [EC1.6.2.2], putative
Schistosoma mansoni Smp_053230   NADH-cytochrome B5 reductase
Schmidtea mediterranea mk4.013765.00   NADH-cytochrome b5 reductase, putative
Schmidtea mediterranea mk4.003572.00  
Trypanosoma brucei gambiense Tbg972.11.4290   NADH-cytochrome b5 reductase, putative
Trypanosoma brucei Tb927.11.3750   NADH-cytochrome b5 reductase, putative
Trypanosoma congolense TcIL3000.11.3700   NADH-cytochrome b5 reductase, putative
Trypanosoma cruzi TcCLB.511859.170   NADH-cytochrome b5 reductase, putative
Trypanosoma cruzi TcCLB.511047.40   NADH-cytochrome b5 reductase, putative
Toxoplasma gondii TGME49_262910   NADH-cytochrome b5 reductase 1, putative
Theileria parva TP02_0545   NADH-cytochrome B5 reductase, putative

Essentiality

TcCLB.511859.170 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.1230 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.1230 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.1230 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.1230 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b1392 Escherichia coli non-essential goodall
CELE_T05H4.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_T05H4.4 Caenorhabditis elegans larval arrest wormbase
CELE_T05H4.4 Caenorhabditis elegans slow growth wormbase
CELE_T05H4.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_T05H4.5 Caenorhabditis elegans larval arrest wormbase
CELE_T05H4.5 Caenorhabditis elegans slow growth wormbase
PBANKA_1143400 Plasmodium berghei Dispensable plasmo
TGME49_262910 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.511859.170 (Trypanosoma cruzi), NADH-cytochrome b5 reductase, putative
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