Detailed view for TcCLB.509777.100

Basic information

TDR Targets ID: 42980
Trypanosoma cruzi, ubiquitin-activating enzyme, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.8192 | Length (AA): 854 | MW (Da): 93950 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00899   ThiF family
PF14732   Ubiquitin/SUMO-activating enzyme ubiquitin-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0019948   SUMO activating enzyme activity  
GO:0016925   protein sumoylation  
GO:0016881   acid-amino acid ligase activity  
GO:0008641   small protein activating enzyme activity  
GO:0005524   ATP binding  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  
GO:0006464   protein modification process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
175 425 1zud (1) 3 245 24.00 0 1 0.6 -0.75
196 774 1y8q (B) 10 550 37.00 0 1 0.75 0.21
2 720 4ii2 (A) 229 1004 20.00 0 1 0.87942 1.23
173 340 1y8q (A) 11 164 23.00 0.00002 1 0.466221 -0.39
196 394 4gsk (A) 317 533 22.00 0 0.99 0.499521 -0.44
197 818 3kyc (B) 11 637 37.00 0 1 0.902837 0.88
198 573 4ii2 (A) 422 866 33.00 0 1 0.601481 0.82
199 792 3kyc (B) 13 623 40.00 0 1 0.90775 0.81
672 769 5fq2 (B) 447 547 20.00 0.0000000000064 0.93 0.380254 -0.62

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127838)

Species Accession Gene Product
Arabidopsis thaliana AT2G21470   SUMO-activating enzyme subunit 2
Babesia bovis BBOV_IV001050   ubiquitin-activating enzyme, putative
Brugia malayi Bm1_53850   ThiF family protein
Candida albicans CaO19.5074   similar to Uba1 (Ub-activating enzyme)
Candida albicans CaO19.12540   similar to Uba1 (Ub-activating enzyme)
Cryptosporidium hominis Chro.20160   SUMO-1 activating enzyme subunit 2
Cryptosporidium parvum cgd2_1460   SUMO-1 activating enzyme subunit 2, putative
Dictyostelium discoideum DDB_G0286919   sumo-activating enzyme subunit 2
Drosophila melanogaster Dmel_CG7528   Smt3 activating enzyme 2
Echinococcus granulosus EgrG_001145300   sumo-activating enzyme subunit 2
Entamoeba histolytica EHI_035540   ubiquitin-activating enzyme, putative
Echinococcus multilocularis EmuJ_001145300   SUMO activating enzyme subunit 2
Giardia lamblia GL50803_6288   Ubiquitin-activating enzyme E1 1
Homo sapiens ENSG00000126261   ubiquitin-like modifier activating enzyme 2
Leishmania braziliensis LbrM.08.0230   ubiquitin-activating enzyme-like protein
Leishmania donovani LdBPK_080230.1   ubiquitin-activating enzyme-like protein
Leishmania infantum LinJ.08.0230   ubiquitin-activating enzyme-like protein
Leishmania major LmjF.08.0220   ubiquitin-activating enzyme-like protein
Leishmania mexicana LmxM.08.0220   ubiquitin-activating enzyme-like protein
Loa Loa (eye worm) LOAG_07505   ThiF family protein
Mus musculus ENSMUSG00000052997   ubiquitin-like modifier activating enzyme 2
Neospora caninum NCLIV_055390   AFR138Wp, related
Oryza sativa 4343750   Os07g0586500
Plasmodium berghei PBANKA_1451600   SUMO-activating enzyme subunit 2, putative
Plasmodium falciparum PF3D7_1237000   SUMO-activating enzyme subunit 2
Plasmodium knowlesi PKNH_1456700   SUMO-activating enzyme subunit 2, putative
Plasmodium vivax PVX_100800   SUMO-activating enzyme subunit 2, putative
Plasmodium yoelii PY05539   related to ubiquitin-activating enzyme homolog UBA2-related
Saccharomyces cerevisiae YDR390C   E1 ubiquitin-activating protein UBA2
Schistosoma japonicum Sjp_0101860   ko:K10685 ubiquitin-like 1-activating enzyme E1 B, putative
Schistosoma mansoni Smp_166220   ubiquitin-activating enzyme E1b
Schmidtea mediterranea mk4.026733.00   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.002146.08   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.003668.01   SUMO-activating enzyme subunit 2
Schmidtea mediterranea mk4.000051.00   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.021673.00   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.015346.00   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.002412.00   SUMO-activating enzyme subunit uba-2
Schmidtea mediterranea mk4.016507.01  
Schmidtea mediterranea mk4.000092.00   SUMO-activating enzyme subunit uba-2
Trypanosoma brucei gambiense Tbg972.5.4810   ubiquitin-activating enzyme e1, putative
Trypanosoma brucei Tb927.5.3430   ubiquitin-activating enzyme E1, putative
Trypanosoma congolense TcIL3000_5_3890   ubiquitin-activating enzyme E1, putative
Trypanosoma cruzi TcCLB.509117.10   ubiquitin-activating enzyme, putative
Trypanosoma cruzi TcCLB.511655.69   ubiquitin-activating enzyme-like protein
Trypanosoma cruzi TcCLB.509777.100   ubiquitin-activating enzyme, putative
Toxoplasma gondii TGME49_311500   ThiF family protein
Theileria parva TP01_0127   ubiquitin-protein ligase, putative
Trichomonas vaginalis TVAG_211530   molybdopterin biosynthesis moeb protein, putative

Essentiality

TcCLB.509777.100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.3430 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.3430 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.3430 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.5.3430 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
YDR390C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1451600 Plasmodium berghei Essential plasmo
TGME49_311500 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens ubiquitin-like modifier activating enzyme 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0057 1 1
0.0038 0.5276 1
0.0038 0.4012 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.509777.100 (Trypanosoma cruzi), ubiquitin-activating enzyme, putative
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