Detailed view for TcCLB.410049.20

Basic information

TDR Targets ID: 43029
Trypanosoma cruzi, cytochrome b5-like Heme/Steroid binding domain containing protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.0978 | Length (AA): 196 | MW (Da): 22141 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00173   Cytochrome b5-like Heme/Steroid binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0020037   heme binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
112 192 1eue (A) 1 81 31.00 0 1 0.92 -1.16
115 190 1cxy (A) 7 86 25.00 0 1 0.75 -1.34
13 55 2a9d (A) 426 465 18.00 0.15 0 0.370188 -0.6
112 194 2ibj (A) 1 83 31.00 0.0000000000064 1 0.861269 -0.47
115 193 3lf5 (A) 54 132 39.00 0 1 0.960861 -0.8
115 193 2keo (A) 23 101 25.00 0 0.99 0.868861 -1.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127870)

Species Accession Gene Product
Arabidopsis thaliana AT5G09680   reduced lateral root formation protein
Babesia bovis BBOV_I004720   cytochrome b5-like Heme/Steroid binding domain containing protein
Brugia malayi Bm1_26075   Cytochrome b5-like Heme/Steroid binding domain containing protein
Candida albicans CaO19.4522   similar to A. thaliana F17I14_130
Candida albicans CaO19.11997   similar to A. thaliana F17I14_130
Caenorhabditis elegans CELE_Y52B11A.3   Protein Y52B11A.3, isoform B
Dictyostelium discoideum DDB_G0275647   cytochrome b5 domain-containing protein
Drosophila melanogaster Dmel_CG11257   CG11257 gene product from transcript CG11257-RA
Giardia lamblia GL50803_9089   Cytochrome B5, outer mitochondrial membrane
Giardia lamblia GL50803_27747   Flavohemoprotein B5+B5R
Giardia lamblia GL50803_33870   Hypothetical protein
Homo sapiens 51167   cytochrome b5 reductase 4
Leishmania braziliensis LbrM.26.1450   hypothetical protein, conserved
Leishmania donovani LdBPK_261410.1   cytochrome b5-like Heme/Steroid binding domain containing protein, putative
Leishmania infantum LinJ.26.1410   hypothetical protein, conserved
Leishmania major LmjF.26.1430   hypothetical protein, conserved
Leishmania mexicana LmxM.26.1430   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_02505   cytochrome b5 reductase 4
Mus musculus ENSMUSG00000032872   cytochrome b5 reductase 4
Neospora caninum NCLIV_056850   hypothetical protein
Oryza sativa 4342780   Os07g0232200
Onchocerca volvulus OVOC1602  
Plasmodium berghei PBANKA_0819100   cytochrome b5, putative
Plasmodium falciparum PF3D7_0918100   cytochrome b5-like heme/steroid binding protein, putative
Plasmodium knowlesi PKNH_0716100   heme binding protein, putative
Plasmodium vivax PVX_099325   cytochrome b5-like Heme/Steroid binding domain containing protein
Plasmodium yoelii PY07000   cytochrome B5, outer mitochondrial membrane
Saccharomyces cerevisiae YMR073C   Irc21p
Schmidtea mediterranea mk4.002226.03   Putative cytochrome b5
Trypanosoma brucei gambiense Tbg972.7.390   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.520   cytochrome b5-like Heme/Steroid binding domain containing protein, putative
Trypanosoma congolense TcIL3000_7_210   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.410049.20   cytochrome b5-like Heme/Steroid binding domain containing protein, putative
Trypanosoma cruzi TcCLB.506287.30   cytochrome b5-like Heme/Steroid binding domain containing protein, putative
Toxoplasma gondii TGME49_313580   cytochrome b5 family heme/steroid binding domain-containing protein
Toxoplasma gondii TGME49_313590   hypothetical protein
Trichomonas vaginalis TVAG_203330   cytochrome B5, putative
Trichomonas vaginalis TVAG_063210   cytochrome B5 isoform 1, putative
Trichomonas vaginalis TVAG_032980   cytochrome B5 isoform 1, putative
Trichomonas vaginalis TVAG_329360   flavohemoprotein B5/b5r, putative
Trichomonas vaginalis TVAG_274060   fatty acid desaturase, putative

Essentiality

TcCLB.410049.20 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.520 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.520 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.520 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.520 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0819100 Plasmodium berghei Slow plasmo
TGME49_313590 Toxoplasma gondii Essentiality uncertain sidik
TGME49_313580 Toxoplasma gondii Essentiality uncertain sidik
TGME49_313590 Toxoplasma gondii Probably essential sidik
TGME49_313580 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TcCLB.410049.20 (Trypanosoma cruzi), cytochrome b5-like Heme/Steroid binding domain containing protein, putative
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