Detailed view for PF3D7_0527300

Basic information

TDR Targets ID: 4304
Plasmodium falciparum, methionine aminopeptidase 1a, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.5231 | Length (AA): 327 | MW (Da): 37411 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00557   Metallopeptidase family M24

Gene Ontology

Mouse over links to read term descriptions.
GO:0004177   aminopeptidase activity  
GO:0008235   metalloexopeptidase activity  
GO:0006508   proteolysis  
GO:0009987   cellular process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 235 1y1n (A) 50 283 40.00 0 1 1.6 -1.43
1 235 2b3h (A) 148 381 43.00 0 1 1.63 -1.37
1 327 2zsg (A) 47 350 20.00 0 1 1.1647 0.23
38 327 3pka (A) 2 283 38.00 0 1 1.45715 -1.18
81 327 1o0x (A) 6 252 35.00 0 1 1.30005 -1.04
83 327 4fo7 (A) 4 248 43.00 0 1 1.32194 -0.55

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, merozoite, sporozoite, early ring, early trophozoite, late ring, late trophozoite, Ring, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, early schizont, late schizont, Oocyst, Sporozoite, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_126721)

Species Accession Gene Product
Arabidopsis thaliana AT2G45240   methionine aminopeptidase 1A
Arabidopsis thaliana AT1G13270   methionine aminopeptidase 1B
Arabidopsis thaliana AT4G37040   methionine aminopeptidase 1D
Babesia bovis BBOV_IV006760   methionine aminopeptidase, putative
Babesia bovis BBOV_III008650   methionine aminopeptidase, putative
Brugia malayi Bm1_31345   Methionine aminopeptidase protein type I
Candida albicans CaO19.10636   methionine aminopeptidase
Candida albicans CaO19.3124   methionine aminopeptidase
Caenorhabditis elegans CELE_Y37E11AL.7   Protein MAP-1
Cryptosporidium hominis Chro.10305   methionine aminopeptidase 1 (MetAP 1) (MAP 1) (Peptidase M 1)
Cryptosporidium parvum cgd1_2700   methionine aminopeptidase with MYND finger at N-terminus
Chlamydia trachomatis CT_851   methionine aminopeptidase
Dictyostelium discoideum DDB_G0279433   methionine aminopeptidase 1
Dictyostelium discoideum DDB_G0280127   methionine aminopeptidase
Drosophila melanogaster Dmel_CG13630   CG13630 gene product from transcript CG13630-RA
Drosophila melanogaster Dmel_CG5188   CG5188 gene product from transcript CG5188-RA
Escherichia coli b0168   methionine aminopeptidase
Echinococcus granulosus EgrG_000923200   methionyl aminopeptidase 1 M24 family
Echinococcus multilocularis EmuJ_000923200   methionyl aminopeptidase 1 (M24 family)
Echinococcus multilocularis EmuJ_000025800   methionyl aminopeptidase 1 (M24 family)
Homo sapiens 254042   methionyl aminopeptidase type 1D (mitochondrial)
Homo sapiens ENSG00000164024   methionyl aminopeptidase 1
Leishmania braziliensis LbrM.19.0860   methionine aminopeptidase, putative,metallo-peptidase, Clan MG, Family M24
Leishmania donovani LdBPK_190540.1   metallo- peptidase, Clan MG, Family M24
Leishmania infantum LinJ.19.0540   methionine aminopeptidase, putative,metallo-peptidase, Clan MG, Family M24
Leishmania major LmjF.19.0550   methionine aminopeptidase, putative,metallo-peptidase, Clan MG, Family M24
Leishmania mexicana LmxM.19.0550   methionine aminopeptidase
Loa Loa (eye worm) LOAG_04834   methionine aminopeptidase type I
Mycobacterium leprae ML1576c   PROBABLE METHIONINE AMINOPEPTIDASE MAPB (MAP) (PEPTIDASE M)
Mycobacterium leprae ML1831c   PROBABLE METHIONINE AMINOPEPTIDASE MAPA (MAP) (PEPTIDASE M) (MetAP)
Mus musculus ENSMUSG00000041921   methionyl aminopeptidase type 1D (mitochondrial)
Mus musculus ENSMUSG00000005813   methionyl aminopeptidase 1
Mycobacterium tuberculosis Rv2861c   Methionine aminopeptidase MapB (map) (peptidase M)
Mycobacterium tuberculosis Rv0734   Methionine aminopeptidase MapA (map) (peptidase M) (MetAP)
Mycobacterium ulcerans MUL_2091   methionine aminopeptidase MapB
Mycobacterium ulcerans MUL_0830   methionine aminopeptidase
Neospora caninum NCLIV_028520   methionine aminopeptidase, putative
Neospora caninum NCLIV_064990   methionine aminopeptidase, putative
Neospora caninum NCLIV_011650   methionine aminopeptidase, putative
Oryza sativa 4330811   Os02g0761700
Oryza sativa 4336948   Os04g0610500
Oryza sativa 4349075   Os10g0508400
Plasmodium berghei PBANKA_1242100   methionine aminopeptidase 1a, putative
Plasmodium berghei PBANKA_1213700   methionine aminopeptidase 1b, putative
Plasmodium falciparum PF3D7_1015300   methionine aminopeptidase 1b, putative
Plasmodium falciparum PF3D7_0527300   methionine aminopeptidase 1a, putative
Plasmodium knowlesi PKNH_1005400   methionine aminopeptidase 1a, putative
Plasmodium knowlesi PKNH_0815400   methionine aminopeptidase 1b, putative
Plasmodium vivax PVX_079875   methionine aminopeptidase 1a, putative
Plasmodium vivax PVX_094985   methionine aminopeptidase 1b, putative
Plasmodium yoelii PY01653   methionine aminopeptidase, type I
Plasmodium yoelii PY04617   methionine aminopeptidase, type I, putative
Saccharomyces cerevisiae YLR244C   Map1p
Schistosoma japonicum Sjp_0097930   ko:K01265 methionyl aminopeptidase [EC3.4.11.18], putative
Schistosoma japonicum Sjp_0305740   ko:K01265 methionyl aminopeptidase [EC3.4.11.18], putative
Schistosoma japonicum Sjp_0108750   Methionine aminopeptidase 1, putative
Schistosoma mansoni Smp_142010.1   methionyl aminopeptidase 1 (M24 family)
Schistosoma mansoni Smp_142010.2   methionyl aminopeptidase 1 (M24 family)
Schmidtea mediterranea mk4.044698.02  
Schmidtea mediterranea mk4.034199.00  
Schmidtea mediterranea mk4.003233.01   Methionine aminopeptidase 1
Trypanosoma brucei gambiense Tbg972.10.18570   methionine aminopeptidase, putative,metallo- peptidase, Clan MG, Family M24
Trypanosoma brucei Tb11.v5.0998   methionine aminopeptidase, putative
Trypanosoma brucei Tb927.10.15270   metallo- peptidase, Clan MG, Family M24
Trypanosoma brucei Tb11.1240   methionine aminopeptidase, type I, putative
Trypanosoma congolense TcIL3000_10_13060   metallo- peptidase, Clan MG, Family M24
Trypanosoma cruzi TcCLB.507031.120   metallo- peptidase, Clan MG, Family M24
Trypanosoma cruzi TcCLB.504111.20   metallo- peptidase, Clan MG, Family M24
Toxoplasma gondii TGME49_248850   methionine aminopeptidase
Toxoplasma gondii TGME49_211330   methionine aminopeptidase
Toxoplasma gondii TGME49_257730   methionine aminopeptidase, type i, putative
Treponema pallidum TP0842   methionine aminopeptidase (map)
Theileria parva TP04_0770   methionine aminopeptidase, type I, putative
Theileria parva TP04_0063   methionine aminopeptidase, type I, putative
Wolbachia endosymbiont of Brugia malayi Wbm0112   methionine aminopeptidase

Essentiality

PF3D7_0527300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu2908 Mycobacterium tuberculosis non-essential nmpdr
Tb927.10.15270 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.15270 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.15270 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.10.15270 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0168 Escherichia coli essential goodall
PBANKA_1213700 Plasmodium berghei Dispensable plasmo
PBANKA_1242100 Plasmodium berghei Essential plasmo
TGME49_211330 Toxoplasma gondii Probably essential sidik
TGME49_248850 Toxoplasma gondii Probably essential sidik
TGME49_257730 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.8

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Staphylococcus aureus (strain COL) Methionine aminopeptidase Compounds References
Saccharomyces cerevisiae Map1p Compounds References
Escherichia coli methionine aminopeptidase Compounds References
Homo sapiens methionyl aminopeptidase 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0086 0.4653 0.795
0.0155 0.7688 0.7688
0.027 0.5909 0.5812
0.0029 0.422 0.422
0.0809 0.5124 0.6018
0.117043 0.766654 0.766654
0.1527 0.8043 0.7907
0.0052 1 1
0.0179 0.6191 0.5
0.0836 0.3527 0.6308
0.0863 0.5004 0.6288
0.0272 0.9254 0.9254
0.1007 0.6223 0.6018
0.0179 0.6191 0.5
0.0036 0.3367 0
0.0873 0.8442 0.8339
0.0041 0.2986 0
0.0122 0.422 0.422
0.1114 0.8416 0.8414
0.0266 0.2931 1
0.091 0.8096 0.8088
0.091 0.8096 0.8088
0.0861968 0.703479 0.763016
0.0816 0.4824 0.4824
0.0262 0.8064 0.8064
0.0146 0.2633 0.2633
0.0044 0.3031 0.5
0.0052 0.6416 0.5
0.1007 0.6223 0.6018
0.1558 0.8094 0.8067
0.1103 0.8442 0.8409
0.0861968 0.703479 0.763016
0.022 0.8017 0.7987
0.076 0.7688 0.7688
0.0861968 0.703479 0.763016
0.0052 1 1
0.022 0.9 1
0.0052 0.5 0.5
0.0148 0.3878 0.7914
0.1052 0.839 0.8278
0.0835 0.5616 0.6259
0.0086 0.2877 0.7907
0.1267 0.8025 0.8025
0.0373 0.2967 0
0.0809 0.5124 0.6018
0.0224 0.8442 0
0.0908 0.8275 0.8268
0.0803 0.5976 0.6402
0.009 0.2643 0.2643
0.013 0.3738 0
0.1133 0.3469 0.8362
0.1089 0.8377 0.8377
0.0861968 0.703479 0.763016
0.1007 0.6223 0.6018
0.0658 0.2999 0.9181
0.0798 0.8363 0.8353
0.0326 0.9411 0.9411
0.0654 0.8166 0.8166
0.0501 0.7898 0.7898
0.0034 0.3356 0
0.0281736 1 1
0.1007 0.6223 0.6018
0.0256 0.2879 0.929
0.0916 0.3717 0
0.0086 0.7688 0.7688
0.0816 0.4824 0.4824
0.0798 0.8363 0.8353
0.0086 0.7688 0.7688
0.0086 0.4078 0.7688
0.0272 0.2994 0.883
0.1356 0.8027 0.8016
0.0036 0.3367 0
0.0932 0.7907 0.7907
0.1144 0.6801 0.8313
0.1007 0.6223 0.6018
0.0148 0.3878 0.7914
0.0093 0.5562 1
0.0046 0.3031 0.5
0.0509 0.2932 0.7688
0.1052 0.839 0.8278
0.0136 0.8555 0.8555
0.1162 0.8339 0.8176
0.0131 1 1
0.003 0.5244 0.422
0.0348 0.4215 1
0.0086 0.3107 0.7688
0.0279 0.5085 0.9322
0.1007 0.6223 0.6018
0.0036 0.3367 0
0.0975 0.8303 0.8303
0.0509 0.2932 0.7688
0.0124 0.4028 0.4028
0.0034 0.3174 0
0.0039 0.3716 0.5141
0.0997 0.8198 0.8198
0.1073 0.6014 0.626
0.0179 0.6191 0.5
0.0028 0.5623 0.422
0.3659 0.8067 0.8067
0.0861968 0.703479 0.763016
0.0146 0.2633 0.2633
0.0052 0.5 0.5
0.0871 0.422 0.422
0.0662 0.8083 0.8061
0.0279 0.5085 0.9322
0.1007 0.6223 0.6018
0.0065 0.2855 0.2855
0.0129 0.411 1
0.0179 0.484 1
0.0145 0.3595 1
0.0153 0.4174 0.5382
0.1021 0.3056 0.8369
0.0156 0.4585 0.7907
0.0676 0.2779 0.8495
0.0835 0.5616 0.6259
0.0052 0.3649 0.5
0.1055 0.3203 0.8193
0.0301 0.6233 0.6218
0.0654 0.8166 0.8166
0.0028 0.5488 0.422
0.0192 0.738 1
0.0333 0.373 0.7907
0.0262 0.3235 1
0.1045 0.8398 0.8351
0.0283 0.3008 1
0.0936 0.3807 0
0.0084 0.512 1
0.0733 0.9039 0.9134
0.0838 0.6654 0.8085
0.0058 0.3999 0.5121
0.0044 0.3031 0.5
0.076 0.7688 0.7688
0.1056 0.3317 0.6018
0.0811 0.3205 0
0.0041 1 0.5
0.0279 0.5085 0.9322
0.0937 0.5217 0.6228
0.0932 0.7907 0.7907
0.0279 0.5085 0.9322
0.0086 0.7457 0
0.0086 0.2874 0
0.0819 0.3163 0.6309
0.0676 0.2779 0.8495
0.0103 0.5 0.5
0.0103 0.5 0.5
0.0085 1 0.5
0.0084 0.2654 1
0.0205 0.5304 0.422
0.0333 0.373 0.7907
0.0047 0.3324 0.5
0.0356 0.3672 1
0.0058 0.5106 0.422
0.0676 0.2779 0.8495
0.0112 0.2881 0.8555
0.0205 0.4007 0.6174
0.0086 0.4653 0.795
0.0088 0.6223 0.6018
0.0367 0.422 0.422
0.0086 0.762 0
0.1049 0.8171 0.8171
0.0037 0.5141 0.5141
0.091 0.8096 0.8088
0.0676 0.2779 0.8495
0.0086 0.4115 0.7688
0.0094 0.4559 0.4559
0.1031 0.8274 0.8274
0.0288 0.2872 0.9383
0.0078 0.7907 0.7907
0.0816 0.4824 0.4824
0.0279 0.5085 0.9322
0.0872 0.7292 0
0.0127 0.5354 1
0.0272 0.2994 0.883
0.0271 0.9236 0.9236
0.0677 0.8495 0.8495
0.0086 0.6778 0
0.1113 0.2847 0.422
0.0114 0.2756 0.8552
0.0086 0.7457 0
0.1162 0.8339 0.8176
0.0029 0.422 0.422
0.0155 0.387 0.422
0.0229 0.3032 0.7688
0.0227 0.2565 1
0.0041 0.2986 0
0.0267 0.2578 1
0.0856 0.4866 0.6209
0.0041 0.7902 0.7688
0.0267 0.2578 1
0.1073 0.5043 0.6018
0.0556 0.422 0.422
0.1055 0.3203 0.8193
0.0066 0.2802 0.2802
0.0816 0.4824 0.4824
0.0836 0.3527 0.6308
0.0148 0.3878 0.7914
0.0205 0.5304 0.422
0.042 0.2954 0.8053
0.1318 0.838 0.8199
0.0034 0.422 0.422
0.0133 0.3326 1
0.0997 0.8198 0.8198
0.2604 0.3145 0.8568
0.0132 0.8332 1
0.0434 0.3695 0
0.0508 0.6886 0.8068
0.022 0.9 1
0.0302 0.6231 0.9423
0.0029 0.2853 0.2443
0.103 0.6135 1
0.1356 0.8027 0.8016
0.0164 0.3646 0.3496
0.1007 0.6223 0.6018
0.0088 0.2994 0.5
0.0863 0.5004 0.6288
0.1045 0.8398 0.8351
0.0809 0.5124 0.6018
0.0029 0.422 0.422
0.0256 0.2879 0.929
0.0863 0.5004 0.6288
0.0039 0.5141 0.5141
0.076 0.7688 0.7688
0.0508 0.6886 0.8068
0.0348 0.4215 1

Assayability

Assay information

  • Assay for Leucine Aminopeptidase, Cytosol (3.4.11.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

9 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_0527300 (Plasmodium falciparum), methionine aminopeptidase 1a, putative
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