Detailed view for LbrM.35.0400

Basic information

TDR Targets ID: 430928
Leishmania braziliensis, proteasome beta 2 subunit, putative
Source Database / ID: 

pI: 6.2164 | Length (AA): 206 | MW (Da): 22849 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00227   Proteasome subunit

Gene Ontology

Mouse over links to read term descriptions.
GO:0005839   proteasome core complex  
GO:0004298   threonine endopeptidase activity  
GO:0051603   proteolysis involved in cellular protein catabolic process  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 203 1ryp (K) 2 192 37.00 0 1 1.43217 -0.6
13 199 5le5 (J) 12 188 43.00 0 1 1.38787 -0.18

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127700)

Species Accession Gene Product
Arabidopsis thaliana AT3G22630   20S proteasome beta subunit D1
Arabidopsis thaliana AT4G14800   20S proteasome beta subunit D2
Babesia bovis BBOV_I004450   proteasome beta 2 subunit, putative
Brugia malayi Bm1_29265   proteasome subunit beta type 2
Candida albicans CaO19.11508   endopeptidase required for mitosis and sporulation
Candida albicans CaO19.4025   endopeptidase required for mitosis and sporulation
Caenorhabditis elegans CELE_T20F5.2   Protein PBS-4
Cryptosporidium hominis Chro.10054   20S proteasome beta subunit D2 (PBD2)
Cryptosporidium parvum cgd1_420   20S proteasome beta subunit D2 (PBD2) , putative
Dictyostelium discoideum DDB_G0269472   20S proteasome subunit beta-2
Drosophila melanogaster Dmel_CG17302   Proteasome beta4 subunit-related 2
Drosophila melanogaster Dmel_CG17331   Proteasome beta4 subunit
Echinococcus granulosus EgrG_000481200   proteasome prosome macropain subunit beta
Entamoeba histolytica EHI_078710   probable proteasome subunit beta type 2, putative
Echinococcus multilocularis EmuJ_000481200   proteasome (prosome, macropain) subunit, beta
Giardia lamblia GL50803_3209   Proteasome subunit beta type 2
Homo sapiens ENSG00000126067   proteasome (prosome, macropain) subunit, beta type, 2
Leishmania braziliensis LbrM.35.0400   proteasome beta 2 subunit, putative
Leishmania donovani LdBPK_360340.1   proteasome subunit beta type-2, putative
Leishmania infantum LinJ.36.0340   proteasome beta 2 subunit, putative
Leishmania major LmjF.36.0320   proteasome beta 2 subunit, putative
Leishmania mexicana LmxM.36.0320   proteasome beta 2 subunit, putative
Loa Loa (eye worm) LOAG_05258   proteasome subunit beta type 2
Mus musculus ENSMUSG00000028837   proteasome (prosome, macropain) subunit, beta type 2
Neospora caninum NCLIV_009170   Proteasome (Prosome, macropain) subunit, beta type, 1, related
Oryza sativa 4333803   Os03g0695600
Plasmodium berghei PBANKA_1334100   proteasome subunit beta type-2, putative
Plasmodium falciparum PF3D7_1470900   proteasome subunit beta type-2, putative
Plasmodium knowlesi PKNH_1210100   proteasome subunit beta type-2, putative
Plasmodium vivax PVX_116925   proteasome subunit beta type-2, putative
Plasmodium yoelii PY06665   proteasome subunit beta type 2
Saccharomyces cerevisiae YER012W   proteasome core particle subunit beta 4
Schistosoma japonicum Sjp_0075650   ko:K02734 20S proteasome subunit beta 4, putative
Schistosoma mansoni Smp_074500.1   proteasome subunit beta 2 (T01 family)
Schistosoma mansoni Smp_074500.2   proteasome subunit beta 2 (T01 family)
Schmidtea mediterranea mk4.000953.08   Proteasome subunit beta type-2
Schmidtea mediterranea mk4.000953.07   Proteasome subunit beta type-2
Trypanosoma brucei gambiense Tbg972.10.5730   proteasome beta 2 subunit, putative,20S proteasome subunit
Trypanosoma brucei Tb927.10.4710   proteasome subunit beta type-2, putative
Trypanosoma congolense TcIL3000_10_3920   proteasome subunit beta type-2, putative
Trypanosoma cruzi TcCLB.503891.100   proteasome subunit beta type-2, putative
Trypanosoma cruzi TcCLB.510287.30   20S proteasome subunit
Toxoplasma gondii TGME49_254900   proteasome subunit beta type 2, putative
Theileria parva TP01_0187   proteasome subunit beta type 2, putative
Trichomonas vaginalis TVAG_408570   Family T1, proteasome beta subunit, threonine peptidase
Trichomonas vaginalis TVAG_403280   Family T1, proteasome beta subunit, threonine peptidase

Essentiality

LbrM.35.0400 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.4710 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.4710 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.4710 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.4710 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T20F5.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_T20F5.2 Caenorhabditis elegans slow growth wormbase
CELE_T20F5.2 Caenorhabditis elegans sterile wormbase
YER012W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1334100 Plasmodium berghei Essential plasmo
TGME49_254900 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) subunit, beta type, 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LbrM.35.0400 (Leishmania braziliensis), proteasome beta 2 subunit, putative
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