Detailed view for LbrM.28.1850

Basic information

TDR Targets ID: 433911
Leishmania braziliensis, zinc finger protein kinase-like
Source Database / ID: 

pI: 6.5215 | Length (AA): 1313 | MW (Da): 142289 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00787   PX domain
PF13924   Lipocalin-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0035091   phosphoinositide binding  
GO:0005524   ATP binding  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 15 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
120 219 1xte (A) 4 96 27.00 0 0.76 0.197861 -0.3
122 243 4ikb (A) 20 134 26.00 0 1 0.270617 -0.72
124 280 5gw0 (A) 108 256 19.00 0 0.94 0.262273 -0.63
125 225 4az9 (A) 2 96 34.00 0.000011 1 0.315823 -0.87
135 219 3p0c (A) 28 100 38.00 0.0069 0.98 0.307037 0.27
280 389 2mqa (A) 29 138 10.00 0 0.01 0.157478 -0.57
481 890 2c0t (A) 83 493 19.00 0 1 0.457162 0.67
524 1014 4tnd (A) 54 541 26.00 0 1 0.483653 0.36
615 1085 3lij (A) 46 508 22.00 0 1 0.41662 0.44
626 934 2r5t (A) 84 374 41.00 0 1 0.504039 -0.23
628 982 4gv1 (A) 143 477 37.00 0 1 0.653273 -0.41
633 1079 5dvr (A) 49 494 28.00 0 1 0.367342 0.95
633 1079 4rgj (A) 68 515 23.00 0 1 0.393342 0.39
960 1052 2lhh (A) 2 110 17.00 0.13 0.08 0.0727302 -0.12
1120 1272 2dre (A) 4 148 30.00 0.026 0.06 0.266827 0.47

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_148825)

Species Accession Gene Product
Leishmania braziliensis LbrM.28.1850   zinc finger protein kinase-like
Leishmania donovani LdBPK_281800.1   differentiation inhibitory kinase, putative
Leishmania infantum LinJ.28.1800   zinc finger protein kinase-like
Leishmania major LmjF.28.1670   zinc finger protein kinase-like
Leishmania mexicana LmxM.28.1670   zinc finger protein kinase-like
Trypanosoma brucei gambiense Tbg972.11.10370   protein kinase,zinc finger protein kinase, putative
Trypanosoma brucei Tb927.11.9270   differentiation inhibitory kinase
Trypanosoma congolense TcIL3000.11.9670   differentiation inhibitory kinase, putative
Trypanosoma congolense TcIL3000_0_28060   differentiation inhibitory kinase
Trypanosoma cruzi TcCLB.510901.200   zinc finger protein kinase, putative

Essentiality

LbrM.28.1850 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.1030 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.1030 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.1030 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.1030 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 32.1% 318 aa Compounds References
Patiria pectinifera Cdc2 300 aa 27.5% 306 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 26.3% 293 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 24.6% 309 aa Compounds References
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 33.0% 318 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 24.7% 312 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 26.3% 297 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 33.3% 318 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LbrM.28.1850 (Leishmania braziliensis), zinc finger protein kinase-like
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