Detailed view for TcCLB.509495.30

Basic information

TDR Targets ID: 43846
Trypanosoma cruzi, trans-sialidase, Group I, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.4558 | Length (AA): 940 | MW (Da): 99501 | Paralog Number: 17

Signal peptide: Y | GPI Anchor: Y | Predicted trans-membrane segments: 2

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13385   Concanavalin A-like lectin/glucanases superfamily
PF13859   BNR repeat-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0004308   exo-alpha-sialidase activity  
GO:0009405   pathogenesis  

Metabolic Pathways

Chagas disease (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
30 662 1n1t (A) 1 634 71.00 0 1 1.62 -1.65
34 664 1ms9 (A) 2 632 95.00 0 1 1.85 -1.85
20 401 4fj6 (A) 153 533 18.00 0.00000075 0.98 0.518083 0.65
34 664 1ms9 (A) 2 632 95.00 0 1 1.85438 -1.63
667 911 2af5 (A) 42 275 13.00 0 0.05 0.263638 -0.34

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 1MR5:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1MS0:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1MS1:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1MS3:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1MS4:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1MS5:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1MS8:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1MS9:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1S0I:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1S0J:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1WCS:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2AH2:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3B69:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3OPZ:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3PJQ:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_130137)

Species Accession Gene Product
Schmidtea mediterranea mk4.000299.20  
Trypanosoma brucei gambiense Tbg972.7.8790   trans-sialidase, putative
Trypanosoma brucei gambiense Tbg972.7.7980   trans-sialidase, putative
Trypanosoma brucei gambiense Tbg972.8.7610   trans-sialidase, putative,neuraminidase, putative
Trypanosoma brucei gambiense Tbg972.5.850   trans-sialidase, putative,neuraminidase, putative
Trypanosoma brucei gambiense Tbg972.11.9090   trans-sialidase, putative
Trypanosoma brucei Tb927.7.6850   trans-sialidase
Trypanosoma brucei Tb927.7.7480   trans-sialidase, putative
Trypanosoma brucei Tb927.7.6830   trans-sialidase, putative
Trypanosoma brucei Tb927.8.7350   trans-sialidase, putative
Trypanosoma brucei Tb927.5.640   trans-sialidase, putative
Trypanosoma brucei Tb927.8.7340   trans-sialidase, putative
Trypanosoma congolense TcIL3000_0_42170   trans-sialidase
Trypanosoma congolense TcIL3000_0_01140   trans-sialidase
Trypanosoma congolense TcIL3000_0_35120   trans-sialidase
Trypanosoma congolense TcIL3000_7_5530   trans-sialidase
Trypanosoma congolense TcIL3000_0_09500   trans-sialidase
Trypanosoma congolense TcIL3000_0_17070   trans-sialidase
Trypanosoma congolense TcIL3000_0_12970   trans-sialidase
Trypanosoma congolense TcIL3000_0_33860   trans-sialidase
Trypanosoma congolense TcIL3000_0_07070   trans-sialidase
Trypanosoma cruzi TcCLB.507085.30   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.510055.10   trans-sialidase, putative
Trypanosoma cruzi TcCLB.508343.10   trans-sialidase, putative
Trypanosoma cruzi TcCLB.505931.30   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.509495.30   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.508089.10   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.509817.50   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.503993.10   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.508501.320   trans-sialidase, putative
Trypanosoma cruzi TcCLB.510787.10   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.506975.80   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.507979.30   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.508859.118   trans-sialidase, putative
Trypanosoma cruzi TcCLB.401569.10   trans-sialidase, putative
Trypanosoma cruzi TcCLB.511323.10   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.508857.30   trans-sialidase, Group I, putative
Trypanosoma cruzi TcCLB.506895.80   trans-sialidase, putative
Trypanosoma cruzi TcCLB.509481.10   trans-sialidase, putative

Essentiality

TcCLB.509495.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.7480 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.7480 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.7480 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.7480 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.7340 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.7340 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.7340 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.7340 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.5.640 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.640 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.640 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.640 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.7.6850 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.6850 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.6850 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.6850 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Clostridium perfringens Sialidase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0031 0.6594 0.6594
0.0324 0.2547 1
0.0144 0.3728 0.7803
0.0033 0.7063 0.6756
0.003 0.6974 0.6595
0.035 0.2815 1
0.0028 0.3979 0.5539
0.003 0.6594 0.6594
0.0034 1 1
0.0029 0.6512 0.6512
0.0028 0.669 0.441
0.003 0.4287 0.5659
0.003 0.268 0.4938
0.003 0.6974 0.6595
0.003 0.6333 0.6265
0.0029 0.6511 0.6511
0.0031 0.8786 0.8786
0.0031 0.4669 0.5777
0.0031 0.2619 0.4898
0.0031 0.2776 0.4998
0.0031 0.6594 0.6594
0.003 0.4431 0.5702
0.0144 0.3728 0.7803
0.0029 0.6234 0.6159
0.0031 0.6593 0.6593
0.0031 0.2622 0.489
0.0324 0.2704 0.6454
0.0029 0.6512 0.6512
0.0123 0.6504 1
0.0355 0.2766 1
0.0035 1 1
0.0324 0.2704 0.6454
0.0034 0.3485 0.5938
0.0031 0.2812 0.5019
0.003 0.4432 0.5702
0.003 0.6579 0.6579
0.0031 0.2776 0.4998
0.0306 0.2516 1
0.0033 0.7063 0.6756
0.003 0.7074 0.7074
0.0031 0.281 0.5018
0.003 0.4287 0.5658
0.0085 0.2595 1
0.0307 0.2501 1
0.0296 0.2628 1
0.003 0.4547 0.574
0.003 0.4408 0.5695

Assayability

Assay information

  • ChEMBL
  • Inhibition of Trypanosoma cruzi trans-Sialidase
  • ChEMBL
  • Inhibition of Trypanosoma cruzi trans-Sialidase at 1 mM by MuNANA assay
  • ChEMBL
  • Inhibition of Trypanosoma cruzi trans-Sialidase at 0.5 mM by MuNANA assay
  • ChEMBL
  • Inhibition of Trypanosoma cruzi trans-Sialidase at 0.125 mM by MuNANA assay
  • ChEMBL
  • Inhibition of Trypanosoma cruzi trans-Sialidase at 0.05 mM by MuNANA assay
  • ChEMBL
  • Inhibition of Trypanosoma cruzi trans-Sialidase at 0.1 mM by MuNANA assay
  • ChEMBL
  • Inhibition of Trypanosoma cruzi trans-Sialidase by MuNANA assay
  • ChEMBL
  • Inhibition of Trypanosoma cruzi trans-Sialidase by TIA assay
  • ChEMBL
  • Inhibition of Trypanosoma cruzi trans-Sialidase at 1 mM by TIA assay

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.509495.30 (Trypanosoma cruzi), trans-sialidase, Group I, putative
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