Detailed view for TcCLB.510879.220

Basic information

TDR Targets ID: 43954
Trypanosoma cruzi, rhodanese-like domain containing protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.7698 | Length (AA): 250 | MW (Da): 28413 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00581   Rhodanese-like domain

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
80 224 2a2k (A) 389 545 12.00 0 0.98 0.52 -0.28
83 193 1e0c (A) 2 121 17.00 0 0.57 0.54 -0.87
29 85 1dov (A) 156 205 38.00 0.42 0.29 0.4701 0.17
103 195 3flh (A) 30 113 24.00 0 0.5 0.5677 -0.68
103 195 3i2v (A) 14 118 22.00 0 0.42 0.5737 -1.12
104 200 2ouc (A) 160 284 23.00 0 1 0.4487 -0.63

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_132602)

Species Accession Gene Product
Echinococcus granulosus EgrG_001179400   centrosomal protein of 41 kDa
Echinococcus multilocularis EmuJ_001179400   centrosomal protein of 41 kDa
Leishmania braziliensis LbrM.20.2590   hypothetical protein, conserved
Leishmania donovani LdBPK_342840.1   rhodanese-like domain containing protein, putative
Leishmania infantum LinJ.34.2840   hypothetical protein, conserved
Leishmania major LmjF.34.3010   hypothetical protein, conserved
Leishmania mexicana LmxM.33.3010   hypothetical protein, conserved
Mus musculus ENSMUSG00000029790   centrosomal protein 41
Schistosoma japonicum Sjp_0000840   similar to Centrosomal protein of 41 kDa, putative
Schistosoma mansoni Smp_144950   Centrosomal protein of 41 kDa (Cep41 protein) (Testis-specificprotein A14 protein)
Schmidtea mediterranea mk4.000977.06   CEP41
Trypanosoma brucei gambiense Tbg972.4.1620   hypothetical protein, conserved
Trypanosoma brucei Tb927.4.1710   rhodanese-like domain containing protein, putative
Trypanosoma congolense TcIL3000_4_1350   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510879.220   rhodanese-like domain containing protein, putative
Trypanosoma cruzi TcCLB.504057.178   rhodanese-like domain containing protein, putative
Trichomonas vaginalis TVAG_017380   conserved hypothetical protein

Essentiality

TcCLB.510879.220 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.1710 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.1710 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.1710 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.1710 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0005 0.3625 0.3625

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.510879.220 (Trypanosoma cruzi), rhodanese-like domain containing protein, putative
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