Detailed view for PF3D7_0604700
Basic information
Plasmodium falciparum, glyoxalase I
Source Database / ID: PlasmoDB | GeneDB | MPMP
pI: 8.5086 |
Length (AA): 307 |
MW (Da): 35838 |
Paralog Number:
0
Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Network
Pfam domains
Gene Ontology
GO:0009438 methylglyoxal metabolic process
GO:0004462 lactoylglutathione lyase activity
Metabolic Pathways
Structural information
Modbase 3D models:
There are 6 models calculated for this protein. More info on
these models, including the
models themselves is available at:
Modbase
| Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
|---|---|---|---|---|---|---|---|---|---|
| 183 | 305 | 2c21 (A) | 4 | 122 | 18.00 | 0 | 0.48 | 0.62 | -0.64 |
| 193 | 307 | 1f9z (A) | 11 | 126 | 20.00 | 0 | 1 | 0.5 | -0.44 |
| 45 | 90 | 2c21 (A) | 3 | 43 | 37.00 | 0.0072 | 0.04 | 0.268537 | 1.14 |
| 52 | 81 | 2rk0 (A) | 5 | 34 | 33.00 | 0.77 | 0.28 | 0.37642 | 0.58 |
| 56 | 113 | 3ghj (A) | 11 | 68 | 24.00 | 0.29 | 0.11 | 0.479325 | -0.59 |
| 58 | 306 | 5d7z (A) | 30 | 281 | 26.00 | 0.0000043 | 1 | 0.999975 | 0.14 |
Help me make sense of these data.
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
Expression
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Upper 80-100% percentile | Oocyst. | Zanghi G |
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Upper 60-80% percentile | intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Female Gametocyte, Male Gametocyte. | Otto TD Lasonder E |
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Mid 40-60% percentile | intra-erythrocytic - 24 hs, Ring. | Otto TD Zanghi G |
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Lower 20-40% percentile | intra-erythrocytic - 0 hs, intra-erythrocytic - 16 hs, Sporozoite. | Otto TD Zanghi G |
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Lower 0-20% percentile | intra-erythrocytic - 8 hs. | Otto TD |
-
Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection. -
Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq. -
Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
Orthologs
Ortholog group members (OG5_166095)
| Species | Accession | Gene Product |
|---|---|---|
| Plasmodium berghei | PBANKA_0103400 | glyoxalase I, putative |
| Plasmodium falciparum | PF3D7_0604700 | glyoxalase I |
| Plasmodium knowlesi | PKNH_1145800 | glyoxalase I, putative |
| Plasmodium vivax | PVX_113400 | glyoxalase I, putative |
| Plasmodium yoelii | PY04823 | hypothetical protein |
Essentiality
Phenotypes and Validation (curated)
Annotated phenotypes:
| Affected Entity | Phenotypic quality | Occurs in | Occurs at | Evidence | Observed in | Drugs/Inhibitors |
|---|---|---|---|---|---|---|
| growth (GO:0040007) | lethal (sensu genetics) (PATO:0000718) | multi-cellular organism (CARO:0000012) | bloodstream stage (PLO:0040) | in vivo inhibition (TDR:00016) | Plasmodium falciparum | 604195 |
| Annotator: | saralph@unimelb.edu.au. | Comment: | 012/Mar/09. | References: | 15843146 8304987 8359410 | |
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Annotated validation
Associated compounds / Druggability
Known modulators for this target
| Compound | Source | Reference |
|---|---|---|
| Curated by TDRTargets | References |
Predicted associations
By orthology with druggable targets
By sequence similarity to non orthologous druggable targets
| Species | Target | Length | Identity | Alignment span | Linked Drugs | Reference |
|---|---|---|---|---|---|---|
| Trypanosoma cruzi | lactoylglutathione lyase-like protein, putative | 141 aa | 29.5% | 129 aa | Compounds | References |
Obtained from network model
Ranking Plot
Putative Drugs List
Assayability
Assay information
- Assay for Glyoxalase I (4.4.1.5 ) Sigma-Aldrich
- Automatic link to known assays based on EC numbers.
- BRENDA Assay
- An enzyme with this EC number or name or sequence has been assayed in Plasmodium falciparum ( 2 )
Reagent availability
- Reagent:
-
Target Type Source Notes PF3D7_0604700 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Plasmodium falciparum ( 1 )
Bibliographic References
7 literature references were collected for this gene.