Detailed view for TcCLB.437545.10

Basic information

TDR Targets ID: 44140
Trypanosoma cruzi, 3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.1255 | Length (AA): 300 | MW (Da): 33743 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 4

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02544   3-oxo-5-alpha-steroid 4-dehydrogenase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016627   oxidoreductase activity, acting on the CH-CH group of donors  
GO:0016021   integral to membrane  
GO:0005737   cytoplasm  
GO:0006629   lipid metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
40 97 3a5p (A) 40 104 33.00 0 0.14 0.273732 1.61

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127767)

Species Accession Gene Product
Arabidopsis thaliana AT3G55360   very-long-chain enoyl-CoA reductase
Babesia bovis BBOV_III001830   3-oxo-5-alpha-steroid 4-dehydrogenase family protein
Brugia malayi Bm1_42555   Synaptic glycoprotein SC2
Candida albicans CaO19.10803   potential steroid 5-alpha-reductase similar to S. pombe SPBC646.07c and to S. cerevisiae TSC13 (YDL015C) ER protein involved in
Candida albicans CaO19.3293   potential steroid 5-alpha-reductase similar to S. pombe SPBC646.07c and to S. cerevisiae TSC13 (YDL015C) ER protein involved in
Caenorhabditis elegans CELE_C15F1.6   Protein ART-1
Cryptosporidium hominis Chro.20132   hypothetical protein
Cryptosporidium parvum cgd2_1200   steroid reductase like intregral membrane protein with 4x transmembrane domains and an ubiquitin domain at its N-terminus
Dictyostelium discoideum DDB_G0270270   synaptic glycoprotein SC2-like protein
Drosophila melanogaster Dmel_CG10849   CG10849 gene product from transcript CG10849-RA
Echinococcus granulosus EgrG_001150000   synaptic glycoprotein sc2
Entamoeba histolytica EHI_076870   3-oxo-5-alpha-steroid 4-dehydrogenase domain-containing protein
Entamoeba histolytica EHI_045030   trans-2,3-enoyl-CoA reductase, putative
Echinococcus multilocularis EmuJ_001150000   synaptic glycoprotein sc2
Giardia lamblia GL50803_88581   Synaptic glycoprotein SC2
Homo sapiens ENSG00000099797   trans-2,3-enoyl-CoA reductase
Leishmania braziliensis LbrM.25.2220   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Leishmania donovani LdBPK_251850.1   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Leishmania infantum LinJ.25.1850   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Leishmania mexicana LmxM.25.1770   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Mus musculus ENSMUSG00000031708   trans-2,3-enoyl-CoA reductase
Neospora caninum NCLIV_014950   trans-2,3-enoyl-CoA reductase, putative
Oryza sativa 4324516   Os01g0150000
Plasmodium berghei PBANKA_0912700   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Plasmodium falciparum PF3D7_1135900   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Plasmodium knowlesi PKNH_0934100   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Plasmodium vivax PVX_092410   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Plasmodium yoelii PY03009   hypothetical protein
Saccharomyces cerevisiae YDL015C   trans-2-enoyl-CoA reductase (NADPH) TSC13
Schistosoma japonicum Sjp_0213210   ko:K10258 enoyl reductase, putative
Schistosoma mansoni Smp_081820.3   synaptic glycoprotein sc2 related
Schistosoma mansoni Smp_081820.1   synaptic glycoprotein sc2 related
Schistosoma mansoni Smp_081820.4   synaptic glycoprotein sc2 related
Schmidtea mediterranea mk4.000087.20   Probable very-long-chain enoyl-CoA reductase art-1
Trypanosoma brucei gambiense Tbg972.3.1700   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Trypanosoma brucei Tb927.3.1840   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Trypanosoma congolense TcIL3000_3_970   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Trypanosoma cruzi TcCLB.437545.10   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Toxoplasma gondii TGME49_285240   3-oxo-5-alpha-steroid 4-dehydrogenase
Theileria parva TP03_0656   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Trichomonas vaginalis TVAG_080870   synaptic glycoprotein sc2, putative
Trichomonas vaginalis TVAG_231320   synaptic glycoprotein sc2, putative
Trichomonas vaginalis TVAG_032770   synaptic glycoprotein sc2, putative

Essentiality

TcCLB.437545.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.1840 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.1840 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.1840 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.1840 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YDL015C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0912700 Plasmodium berghei Essential plasmo
TGME49_285240 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0283 0.3836 0.2554
0.0496 0.3368 0.3368
0.0309 0.5 0.5
0.0159 0.266 0.2658
0.0996 0.3364 0.3812
0.0404 0.4171 0.4519
0.0097 1 0.5
0.1554 0.3544 0.5809
0.1682 0.5565 0.5565
0.0155 1 0.5
0.0304 0.4297 1
0.01 1 0.5
0.0048 0.7671 0
0.005 0.5523 0.5523
0.0135 0.2869 0.2869
0.0211 0.5639 0
0.0844 0.5869 0.5869
0.008 0.3061 0
0.0043 1 0.5
0.1483 0.6596 0.6588
0.0143 0.5042 0.5042
0.4138 0.2589 0.2588
0.1554 0.3544 0.5809
0.0101 1 0.5
0.0105 1 0.5
0.4138 0.2589 0.2588
0.0359 0.3037 0.2911
0.0043 1 0.5
0.4138 0.2589 0.2588
0.0223 0.4122 0.3919
0.1629 0.5572 0.5572
0.0404 0.4171 0.4519
0.0256 0.4166 0.4163
0.0151 0.5 0.5
0.0307 0.2535 0.46
0.1687 0.5672 0.5668
0.0933 0.3572 0.5129
0.0104 1 0.5
0.0122 0.3443 0.3513
0.008 0.3061 0
0.0102 1 1
0.1571 0.5874 0.5871
0.0392 0.322 0.322
0.0269 0.4728 0.4507
0.4318 0.2522 0.2522
0.0088 0.2846 0.3194
0.0342 0.2831 0.4277
0.0307 0.2535 0.46
0.0047 1 0.5
0.0266 0.3291 0.3247
0.0113 0.4246 0
0.1581 0.5481 0
0.1096 0.5261 0
0.0044 1 0.5
0.0933 0.3572 0.5129
0.0444 0.6168 0.6164
0.3311 0.2651 0.2651
0.0201 0.4631 1
0.0496 0.3494 0.3469
0.0043 1 0.5
0.1703 0.5236 0
0.0338 0.3879 0.5121
0.0051 1 0.5
0.0309 0.5 0.5
0.0184 0.2514 1
0.4318 0.252 0.2517
0.0338 0.3879 0.5121
0.0309 0.5 0.5
0.032 0.2562 0.4639
0.009 0.297 1
0.1711 0.5682 0.5682
0.3121 0.2582 0.2579
0.008 0.3061 0
0.062 0.2871 0.4445
0.1629 0.5572 0.5572
0.1476 0.6522 0.6522
0.0255 0.3177 0.3294
0.1682 0.5565 0.5565
0.4035 0.2725 0.2723
0.1622 0.5875 0.5875
0.009 0.3115 0.2183
0.0044 1 1
0.0201 1 0.5
0.0044 1 1
0.0194 1 0.5
0.0053 0.5 0.5
0.0098 1 0.5
0.0279 0.4126 0.4126
0.0043 0.5 0.5
0.0119 0.3398 0.3033
0.0307 0.4445 0.5186
0.0195 0.2748 1
0.1554 0.3544 0.5809
0.1687 0.5672 0.5668
0.0366 0.456 0.5582
0.4138 0.2589 0.2588
0.1591 0.424 0.4237
0.0433 0.612 1
0.0207 1 0.5
0.0538 0.5231 0
0.0053 0.3812 0.5
0.0555 0.3152 0.3234
0.0259 0.3916 0
0.0658 0.3619 0.3617
0.0308 0.6156 0
0.0044 1 0.5
0.1682 0.5565 0.5565
0.051 0.578 0
0.0053 1 0.5
0.0045 1 0.5
0.0044 0.5 0.5
0.1581 0.5481 0
0.0996 0.3364 0.3812
0.0042 1 0.5
0.1571 0.5874 0.5871
0.0097 1 0.5
0.0129 0.289 0.2637
0.079 0.4926 0
0.4035 0.2725 0.2723
0.0192 0.2683 1
0.0066 0.3029 0
0.071 0.3797 0.3767
0.0444 1 1
0.1682 0.5565 0.5565
0.0044 1 1
0.1711 0.5682 0.5682
0.005 0.257 0.5
0.2756 0.2602 0.2616
0.0305 0.3463 0.3912
0.0499 0.2718 0.3593
0.1554 0.3544 0.5809
0.0386 0.5441 1
0.022 1 1
0.0044 1 0.5
0.0989 0.3014 0.6243
0.0076 0.3029 0
0.1554 0.3544 0.5809
0.0198 1 0.5
0.0253 0.3363 1
0.2295 0.6163 0.6163
0.0051 1 0.5
0.0933 0.3572 0.5129
0.0538 0.5231 0
0.0043 1 0.5
0.0304 0.4297 1
0.015 0.4363 0
0.0043 1 0.5
0.0126 0.4083 0
0.35 0.2629 0.2629
0.1687 0.5672 0.5668
0.1046 0.4051 0.4046
0.005 0.5344 0
0.0301 0.5 0.5
0.0378 0.4707 0.4766
0.0051 1 0.5
0.0366 0.3132 0.3019
0.1554 0.3544 0.5809
0.0137 0.2726 1
0.3121 0.2582 0.2579
0.0291 0.3261 0.3713
0.0043 0.3597 0
0.1096 0.5261 0
0.03 0.2511 0.3936
0.0051 1 0.5
0.0488 0.2858 0.3267
0.1629 0.5572 0.5572
0.0088 0.2846 0.3194
0.1516 0.286 0.6184
0.0211 0.5639 0
0.0255 0.3177 0.3294
0.049 0.2727 0.3285
0.0555 0.3152 0.3234
0.1058 0.3216 0.3958
0.0043 1 0.5
0.0042 1 0.5
0.0044 0.2692 0.5
0.0195 0.5927 0
0.0513 0.7658 0.7658
0.0844 0.5869 0.5869
0.0433 0.612 1
0.0093 0.5 0.5
0.0355 0.5 0.5
0.4259 0.2536 0.2526
0.1554 0.3544 0.5809
0.1554 0.3544 0.5809
0.0359 0.3037 0.2911
0.0229 0.5 0.5
0.0126 0.4331 0.4331
0.4138 0.2589 0.2588
0.049 0.2727 0.3285
0.0394 0.681 0
0.0401 0.2707 0.2707
0.0668 0.2557 0.3998
0.0043 1 0.5
0.0295 0.5 0.5
0.1554 0.3544 0.5809
0.1243 0.5928 0
0.0213 0.6298 0
0.0066 0.301 0.2866
0.005 1 0.5
0.1654 0.4908 0
0.0053 0.5 0.5
0.0132 0.4213 0.5
0.023 0.2518 0.4039
0.0051 1 0.5
0.0113 0.2725 0.4208
0.02 0.2564 0.3448
0.0053 1 0.5
0.0299 0.3146 0.3146
0.0153 1 0.5
0.0099 1 0.5
0.0044 0.5714 0.5
0.0045 0.2685 0.5
0.0135 0.3062 1
0.0051 0.8747 0.5
0.0052 0.4576 0.5
0.0342 0.5715 0
0.0042 0.3015 0.5
0.1554 0.3544 0.5809
0.0066 0.3029 0
0.0652 0.5389 0
0.0411 0.5047 0.5901

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.437545.10 (Trypanosoma cruzi), 3-oxo-5-alpha-steroid 4-dehydrogenase, putative
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