Detailed view for TcCLB.507509.60

Basic information

TDR Targets ID: 45058
Trypanosoma cruzi, WLM domain containing protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.4442 | Length (AA): 539 | MW (Da): 59887 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00641   Zn-finger in Ran binding protein and others
PF08325   WLM domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0008270   zinc ion binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
13 129 5jig (A) 5 127 31.00 0.000000023 0.99 0.681369 -1.24
185 215 4f0a (B) 108 135 46.00 0.85 0.29 0.337814 1
235 264 1nj3 (A) 2 31 43.00 0.077 0.73 0.542959 0.07
237 269 2mxv (A) 7 39 27.00 0 0.49 0.446624 -0.81
466 496 2wwz (C) 662 691 40.00 0.011 0.57 0.383814 0.92

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_131387)

Species Accession Gene Product
Arabidopsis thaliana AT1G55915   zinc ion binding protein
Candida albicans CaO19.7923   weak suppressor of smt3
Candida albicans CaO19.291   weak suppressor of smt3
Leishmania braziliensis LbrM.32.1470   hypothetical protein, conserved
Leishmania donovani LdBPK_321370.1   WLM domain containing protein, putative
Leishmania infantum LinJ.32.1370   hypothetical protein, conserved
Leishmania major LmjF.32.1310   hypothetical protein, conserved
Leishmania mexicana LmxM.31.1310   hypothetical protein, conserved
Oryza sativa 4340366   Os06g0191200
Plasmodium berghei PBANKA_1207800   metalloprotease, putative
Plasmodium falciparum PF3D7_1009500   metalloprotease, putative
Plasmodium knowlesi PKNH_0809200   metalloprotease, putative
Plasmodium vivax PVX_094690   hypothetical protein, conserved
Plasmodium yoelii PY02534   hypothetical protein
Plasmodium yoelii PY02535   hypothetical protein
Saccharomyces cerevisiae YHR134W   Wss1p
Trypanosoma brucei gambiense Tbg972.11.16260   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.14530   WLM domain containing protein, putative
Trypanosoma congolense TcIL3000.11.14810   WLM domain containing protein, putative
Trypanosoma cruzi TcCLB.506871.110   WLM domain containing protein, putative
Trypanosoma cruzi TcCLB.507509.60   WLM domain containing protein, putative

Essentiality

TcCLB.507509.60 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.6130 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.6130 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.6130 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.6130 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1207800 Plasmodium berghei Dispensable plasmo
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0273 0.3426 1
0.0577 0.3257 0.7453
0.049 0.3322 0.5
0.0396 0.3457 0.447
0.0311 0.3115 0.8719
0.0259 0.3438 0.5
0.0169 0.2843 0.5
0.0273 0.2964 0.933
0.0445 0.3486 1
0.0368 0.2849 0.5
0.0492 0.35 1
0.0353 0.3484 1
0.0334 0.3513 1
0.0455 0.2669 1
0.0109 0.3407 0.5
0.0307 0.3438 1
0.0381 0.3359 0.5
0.0113 0.3091 1
0.0209 0.3348 0.5645
0.0476 0.3438 1
0.0273 0.3426 1
0.0343 0.3529 1
0.016 0.3422 1
0.0331 0.3483 1
0.0343 0.3464 0.7623
0.0037 0.2654 1
0.0396 0.3457 0.447
0.0349 0.3246 1
0.0285 0.3113 0
0.0577 0.3352 1
0.0285 0.3113 0
0.0093 0.2786 1
0.0063 0.3204 0.5
0.0521 0.34 1
0.0203 0.2977 0.5
0.0349 0.3246 1
0.0361 0.3397 1
0.0129 0.2612 0.5
0.014 0.315 1
0.0024 0.2575 1
0.0256 0.2563 0.5
0.0085 0.2846 1
0.0418 0.3438 1
0.0131 0.2515 0.5
0.0214 0.2745 0.5
0.0176 0.3438 1
0.0068 0.264 1
0.0296 0.3477 1
0.0117 0.2688 1
0.0347 0.3424 0.5
0.0219 0.2903 0.3343
0.0129 0.2501 1
0.0453 0.3529 1
0.0273 0.2964 0.933
0.0416 0.3472 1
0.0048 0.2849 1
0.0085 0.2648 0.7612
0.0354 0.345 1
0.0082 0.3426 1
0.049 0.3157 1
0.0337 0.3431 0.5
0.0521 0.3438 1
0.054 0.2542 0.5
0.0212 0.3148 1
0.0229 0.3337 1
0.0256 0.2563 0.5
0.013 0.3049 1
0.0127 0.3436 1
0.0179 0.2669 0.826
0.041 0.3428 1
0.0579 0.3385 1
0.0538 0.269 0.269
0.0538 0.269 0.269
0.0138 0.3225 1
0.0527 0.3375 1
0.0445 0.3486 1
0.0556 0.3051 0.5
0.0445 0.3486 1
0.0433 0.3324 1
0.0036 0.2947 1
0.0493 0.352 1
0.0368 0.2526 1
0.0403 0.3537 1
0.0533 0.2913 0.5
0.0451 0.324 1
0.0219 0.2903 0.3343
0.0538 0.269 0.269
0.0493 0.352 1
0.0488 0.3448 1
0.0467 0.305 1
0.0448 0.3057 1
0.0349 0.3246 1
0.0043 0.3043 0.5
0.0238 0.3438 0.5
0.0189 0.3334 0.5
0.054 0.3513 1
0.0139 0.2681 0.5
0.0396 0.3457 0.447
0.0117 0.2688 1
0.0056 0.2663 1
0.0343 0.3464 0.7623
0.0552 0.2987 0.5
0.0261 0.3061 0.8257
0.0155 0.2763 0.5
0.0185 0.3331 1
0.0255 0.321 1
0.0232 0.3204 1
0.0293 0.2686 0.5
0.0448 0.3057 1
0.0459 0.3397 1
0.0355 0.3433 1
0.0504 0.3096 1
0.0394 0.2708 1
0.0232 0.3204 1
0.0443 0.334 0.5
0.0396 0.3457 0.447
0.0432 0.3438 1
0.0493 0.352 1
0.0247 0.3217 1
0.032 0.3438 1
0.0076 0.2765 1
0.0493 0.352 1
0.012 0.3388 1
0.0521 0.34 1
0.0347 0.3424 0.5
0.0273 0.2964 0.933
0.0375 0.3505 1
0.0319 0.3391 0.5
0.0534 0.3528 1
0.0039 0.2989 1
0.0304 0.3509 0.6713
0.00665706 0.325018 1
0.0534 0.3528 1
0.0138 0.2666 0.5
0.0152 0.2714 0.5
0.0416 0.3472 1
0.0378 0.3425 1
0.0469 0.3466 1
0.0365 0.2915 1
0.0092 0.3412 0.939
0.049 0.3157 1
0.0219 0.2903 0.3343
0.0489 0.345 1
0.0505 0.3488 0.858
0.0509 0.3457 1
0.0476 0.3254 0.5
0.0445 0.3486 1
0.0164 0.3128 0.5
0.0023 0.3148 1
0.0219 0.2903 0.3343
0.0542 0.3099 1
0.0129 0.3347 0.5
0.012 0.3388 1
0.0286 0.3142 1
0.0207 0.2745 1
0.0466 0.2741 1
0.0544 0.3142 1
0.0489 0.306 0.5
0.0273 0.2964 0.933
0.0219 0.2903 0.3343
0.0127 0.3436 1
0.0138 0.3225 1
0.0311 0.3115 0.8719
0.0228 0.3438 1
0.0111 0.2753 0.5
0.0521 0.3438 1
0.0071 0.2779 1
0.0355 0.3433 1
0.0029 0.2659 1
0.0134 0.2618 0.5
0.0339 0.3438 1
0.0311 0.3239 0.5
0.0129 0.2501 1
0.0209 0.3348 0.5645
0.0351 0.3239 0.5
0.0422 0.2829 1
0.0044 0.2766 0
0.0133 0.3027 1
0.0201 0.282 1
0.0022 0.2793 0.5
0.0566 0.3159 1
0.0133 0.3027 1
0.0138 0.3349 1
0.008 0.3346 0.5
0.0443 0.334 0.5
0.0175 0.3372 1
0.033 0.3178 1
0.0037 0.2654 1
0.0123 0.293 1
0.0505 0.3488 0.858
0.0259 0.2982 1
0.0142 0.3105 1
0.0176 0.3337 1
0.0273 0.2964 0.933
0.0282 0.3434 0.5
0.0401 0.2719 1
0.0492 0.3407 1
0.0058 0.3143 0.5
0.0313 0.3521 1
0.0323 0.3234 1
0.0354 0.345 1
0.0304 0.2781 1
0.0445 0.3486 1
0.0129 0.2501 1
0.0228 0.2543 1
0.0516 0.3074 1
0.0203 0.2647 0.5
0.0486 0.3438 1
0.0521 0.34 1
0.0288 0.352 1
0.038 0.3496 1
0.0568 0.3496 1
0.0311 0.3264 1
0.0455 0.2669 1
0.0313 0.285 1
0.032 0.3438 1
0.0036 0.2882 1
0.0102475 0.280464 1
0.0311 0.3264 1
0.0258 0.3422 0.5
0.017 0.2828 0.5
0.0219 0.2903 0.3343
0.0127 0.2969 0.5
0.043 0.2664 1
0.0532 0.2616 0.5
0.0382 0.3427 1
0.0254 0.3313 1
0.041 0.3449 1
0.0043 0.2685 0.6993
0.0247 0.2525 0.5
0.0319 0.3438 1
0.0327 0.3261 1
0.0469 0.3466 1
0.032 0.3438 1
0.0274 0.296 1
0.032 0.3438 1
0.0254 0.2634 1
0.0034 0.2564 0.5
0.0245 0.258 0.2536
0.0304 0.3509 0.6713
0.0254 0.2634 1
0.0113 0.3091 1
0.0123 0.3321 0.5
0.0558 0.2532 1
0.0133 0.3394 0
0.0498 0.3475 1
0.0273 0.2964 0.933
0.0501 0.3438 1
0.0579 0.3402 1
0.0155 0.2818 0.5
0.0363 0.3359 1
0.0382 0.3427 1
0.0336 0.3214 1
0.0349 0.3468 1
0.0395 0.3288 1
0.0331 0.3483 1
0.0189 0.3334 0.5
0.0307 0.3438 1
0.0375 0.3505 1
0.0209 0.3348 0.5645
0.0096 0.336 1
0.0138 0.3349 1
0.0037 0.2654 1
0.0219 0.2903 0.3343
0.0219 0.2903 0.3343
0.0414 0.3472 1
0.0353 0.3507 1
0.0422 0.2829 1
0.0128 0.3018 1
0.0285 0.3113 0
0.0247 0.2531 0.5
0.0306 0.2511 0.7899
0.0492 0.3407 1
0.0261 0.3061 0.8257
0.0456 0.3409 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TcCLB.507509.60 (Trypanosoma cruzi), WLM domain containing protein, putative
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