Detailed view for TcCLB.511365.70

Basic information

TDR Targets ID: 46830
Trypanosoma cruzi, proteasome regulatory non-ATP-ase subunit 9, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.48 | Length (AA): 402 | MW (Da): 45513 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01399   PCI domain

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 365 2mr3 (A) 7 356 22.00 0 1 1.12596 0.36
14 152 2mqw (A) 12 159 17.00 0 0.57 0.450671 0.11

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127891)

Species Accession Gene Product
Arabidopsis thaliana AT5G45620   proteasome component (PCI) domain-containing protein
Arabidopsis thaliana AT4G19006   proteasome component (PCI) domain-containing protein
Babesia bovis BBOV_II003010   PCI domain containing protein
Brugia malayi Bm1_19590   PCI domain containing protein
Candida albicans CaO19.9545   similar to S. cerevisiae RPN9 (YDR427W) subunit of the 19S regulatory particle of the 26S proteasome
Candida albicans CaO19.1993   similar to S. cerevisiae RPN9 (YDR427W) subunit of the 19S regulatory particle of the 26S proteasome
Caenorhabditis elegans CELE_T06D8.8   Protein RPN-9
Cryptosporidium hominis Chro.20114   26S proteasome subunit P40.5
Cryptosporidium parvum cgd2_1020   proteasome regulatory subunit Rpn9, PINT domain
Dictyostelium discoideum DDB_G0285105   26S proteasome non-ATPase regulatory subunit 13
Drosophila melanogaster Dmel_CG10230   Regulatory particle non-ATPase 9
Echinococcus granulosus EgrG_001148200   26S proteasome regulatory subunit N1
Entamoeba histolytica EHI_103850   proteasome regulatory subunit, putative
Echinococcus multilocularis EmuJ_001148200   26S proteasome regulatory subunit N1
Homo sapiens ENSG00000185627   proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
Leishmania braziliensis LbrM.19.1440   proteasome regulatory non-ATP-ase subunit, putative
Leishmania donovani LdBPK_191100.1   proteasome regulatory non-ATP-ase subunit 9, putative
Leishmania infantum LinJ.19.1100   proteasome regulatory non-ATP-ase subunit, putative
Leishmania major LmjF.19.1120   proteasome regulatory non-ATP-ase subunit, putative
Leishmania mexicana LmxM.19.1120   proteasome regulatory non-ATP-ase subunit, putative
Loa Loa (eye worm) LOAG_14027   hypothetical protein
Loa Loa (eye worm) LOAG_13755   PCI domain-containing protein
Mus musculus 23997   proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
Neospora caninum NCLIV_002680   AGAP001651-PA, related
Oryza sativa 4324031   Os01g0511300
Oryza sativa 4332055   Os03g0214600
Plasmodium berghei PBANKA_0514500   26S proteasome regulatory subunit RPN9, putative
Plasmodium falciparum PF3D7_1030500   26S proteasome regulatory subunit RPN9, putative
Plasmodium knowlesi PKNH_0615200   26S proteasome regulatory subunit RPN9, putative
Plasmodium vivax PVX_111200   26S proteasome regulatory subunit RPN9, putative
Plasmodium yoelii PY00527   26S proteasome subunit P40.5, putative
Saccharomyces cerevisiae YDR427W   proteasome regulatory particle lid subunit RPN9
Schistosoma japonicum Sjp_0209080   ko:K03039 26S proteasome regulatory subunit N9, putative
Schistosoma mansoni Smp_178810   hypothetical protein
Schmidtea mediterranea mk4.017512.00   26S proteasome non-ATPase regulatory subunit 13
Schmidtea mediterranea mk4.004635.01   26S proteasome non-ATPase regulatory subunit 13
Schmidtea mediterranea mk4.004635.00   26S proteasome non-ATPase regulatory subunit 13
Trypanosoma brucei gambiense Tbg972.10.19250   proteasome regulatory non-ATP-ase subunit 9,19S proteasome regulatory subunit, putative
Trypanosoma brucei Tb927.10.15720   proteasome regulatory non-ATP-ase subunit 9
Trypanosoma congolense TcIL3000_10_13500   proteasome regulatory non-ATP-ase subunit 9, putative
Trypanosoma cruzi TcCLB.511365.70   proteasome regulatory non-ATP-ase subunit 9, putative
Toxoplasma gondii TGME49_207770   PCI domain-containing protein
Toxoplasma gondii TGME49_224560   hypothetical protein
Theileria parva TP04_0292   26S proteasome regulatory subunit, putative
Trichomonas vaginalis TVAG_193070   regulatory particle non-ATPase, putative
Trichomonas vaginalis TVAG_379960   26S proteasome subunit, putative

Essentiality

TcCLB.511365.70 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.15720 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.15720 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.15720 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.15720 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T06D8.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_T06D8.8 Caenorhabditis elegans larval arrest wormbase
CELE_T06D8.8 Caenorhabditis elegans slow growth wormbase
YDR427W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0514500 Plasmodium berghei Essential plasmo
TGME49_207770 Toxoplasma gondii Probably essential sidik
TGME49_224560 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TcCLB.511365.70 (Trypanosoma cruzi), proteasome regulatory non-ATP-ase subunit 9, putative
Title for this comment
Comment