Detailed view for TcCLB.503579.100

Basic information

TDR Targets ID: 47980
Trypanosoma cruzi, nucleotide-binding protein, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6387 | Length (AA): 348 | MW (Da): 37989 | Paralog Number: 5

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF10609   NUBPL iron-transfer P-loop NTPase

Gene Ontology

Mouse over links to read term descriptions.
GO:0051539   4 iron, 4 sulfur cluster binding  
GO:0005524   ATP binding  
GO:0016226   iron-sulfur cluster assembly  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
82 341 2afh (E) 2 249 18.00 0 1 0.95 -0.47
83 339 1hyq (A) 3 232 22.00 0 1 0.99 -1.07
83 340 1hyq (A) 3 233 24.00 0 1 1.05 -0.86
72 324 2ph1 (A) 8 248 39.00 0.0000000078 1 1.22341 -0.57
72 325 3kb1 (A) 8 250 38.00 0 1 1.13079 -0.41
74 107 1f48 (A) 318 351 44.00 0.29 0.72 0.510801 0.93
84 344 1wcv (1) 8 247 21.00 0 0.84 0.9919 -0.64
84 337 3q9l (A) 4 248 25.00 0.0000043 1 0.957985 -0.34
85 336 1hyq (A) 5 232 26.00 0.000000026 1 1.01924 -0.51

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_126620)

Species Accession Gene Product
Arabidopsis thaliana AT3G24430   protein high chlorophyll fluorescence 101
Arabidopsis thaliana AT4G19540   iron-sulfur protein required for NADH dehydrogenase
Arabidopsis thaliana AT5G50960   nucleotide binding protein 35
Babesia bovis BBOV_I000220   conserved hypothetical protein
Babesia bovis BBOV_IV002290   nucleotide-binding protein 1, putative
Brugia malayi Bm1_36105   Mrp-family putative nucleotide-binding protein F10G8.6, putative
Candida albicans CaO19.8965   similar to conserved putative nucleotide binding protein
Candida albicans CaO19.9591   similar to uncharacterized conserved nucleotide binding protein found outside of yeast
Candida albicans CaO19.8367   N terminus of NBP35-like orf
Candida albicans CaO19.8366   C terminus of NBP35-like orf
Candida albicans CaO19.1387   similar to conserved putative nucleotide binding protein
Candida albicans CaO19.747   similar to essential evolutionarily conserved protein related to bacterial plasmid partitioning ATPases
Candida albicans CaO19.2043   similar to uncharacterized conserved nucleotide binding protein found outside of yeast
Caenorhabditis elegans CELE_F10G8.6   Protein NUBP-1
Cryptosporidium hominis Chro.50297   nucleotide-binding protein
Cryptosporidium hominis Chro.80366   hypothetical protein
Cryptosporidium parvum cgd8_3140   MRP like MinD family ATpase of the SIMIBI class of P-loop GTpases
Cryptosporidium parvum cgd5_900   MRP like MinD family ATpase
Dictyostelium discoideum DDB_G0277437   nucleotide binding protein 1
Dictyostelium discoideum DDB_G0277157   nucleotide binding protein 2
Dictyostelium discoideum DDB_G0291193   Mrp/NBP35 family protein
Dictyostelium discoideum DDB_G0285329   nucleotide binding protein 1-like protein
Drosophila melanogaster Dmel_CG4858   CG4858 gene product from transcript CG4858-RA
Drosophila melanogaster Dmel_CG3262   CG3262 gene product from transcript CG3262-RE
Drosophila melanogaster Dmel_CG17904   CG17904 gene product from transcript CG17904-RA
Escherichia coli b2113   antiporter inner membrane protein
Echinococcus granulosus EgrG_000061000   cytosolic Fe S cluster assembly factor nubp1
Echinococcus granulosus EgrG_000126930   putative nucleotide binding protein
Echinococcus granulosus EgrG_000126920   cytosolic Fe S cluster assembly factor NUBP2
Echinococcus granulosus EgrG_001016900   nucleotide binding protein
Entamoeba histolytica EHI_047750   Nucleotide-binding protein , putative
Entamoeba histolytica EHI_000610   nucleotide binding protein 2, putative
Echinococcus multilocularis EmuJ_001016900   nucleotide binding protein
Echinococcus multilocularis EmuJ_000061000   cytosolic Fe S cluster assembly factor nubp1
Echinococcus multilocularis EmuJ_000126910  
Giardia lamblia GL50803_15324   Nucleotide-binding protein 1
Giardia lamblia GL50803_10969   Nucleotide-binding protein 1
Homo sapiens ENSG00000103274   nucleotide binding protein 1
Homo sapiens ENSG00000151413   nucleotide binding protein-like
Homo sapiens ENSG00000095906   nucleotide binding protein 2
Leishmania braziliensis LbrM.21.0720   nucleotide-binding protein, putative
Leishmania braziliensis LbrM.26.0450   nucleotide binding protein-like protein
Leishmania braziliensis LbrM.28.2670   MRP protein-like protein
Leishmania donovani LdBPK_210720.1   nucleotide-binding protein, putative
Leishmania donovani LdBPK_282660.1   MRP protein-like protein
Leishmania donovani LdBPK_260420.1   nucleotide binding protein-like protein
Leishmania infantum LinJ.26.0420   nucleotide binding protein-like protein
Leishmania infantum LinJ.21.0720   nucleotide-binding protein, putative
Leishmania infantum LinJ.28.2660   MRP protein-like protein
Leishmania major LmjF.28.2470   MRP protein-like protein
Leishmania major LmjF.26.0430   nucleotide binding protein-like protein
Leishmania major LmjF.21.0660   nucleotide-binding protein, putative
Leishmania mexicana LmxM.26.0430   nucleotide binding protein-like protein
Leishmania mexicana LmxM.21.0660   nucleotide-binding protein, putative
Leishmania mexicana LmxM.28.2470   MRP protein-like protein
Loa Loa (eye worm) LOAG_05712   hypothetical protein
Mycobacterium leprae ML1080c   PROBABLE MRP-RELATED PROTEIN MRP
Mus musculus ENSMUSG00000039183   nucleotide binding protein 2
Mus musculus 76826   nucleotide binding protein-like
Mus musculus ENSMUSG00000022503   nucleotide binding protein 1
Mycobacterium tuberculosis Rv1229c   Probable Mrp-related protein Mrp
Mycobacterium ulcerans MUL_4515   Mrp-related protein Mrp
Neospora caninum NCLIV_010950   ATP-binding protein, Mrp/Nbp35 family, related
Neospora caninum NCLIV_019460   hypothetical protein
Oryza sativa 4329891   Os02g0601100
Oryza sativa 4324700   Os01g0719700
Oryza sativa 9271868   Os03g0627300
Plasmodium berghei PBANKA_0919700   conserved protein, unknown function
Plasmodium berghei PBANKA_0811900   cytosolic Fe-S cluster assembly factor NBP35, putative
Plasmodium falciparum PF3D7_0910800   cytosolic Fe-S cluster assembly factor NBP35, putative
Plasmodium falciparum PF3D7_1128500   conserved protein, unknown function
Plasmodium knowlesi PKNH_0926600   conserved protein, unknown function
Plasmodium knowlesi PKNH_0708800   cytosolic Fe-S cluster assembly factor NBP35, putative
Plasmodium vivax PVX_098980   cytosolic Fe-S cluster assembly factor NBP35, putative
Plasmodium vivax PVX_092045   hypothetical protein, conserved
Plasmodium yoelii PY03468   nucleotide-binding protein
Plasmodium yoelii PY00852   nucleotide-binding protein
Saccharomyces cerevisiae YGL091C   Nbp35p
Saccharomyces cerevisiae YIL003W   Cfd1p
Schistosoma japonicum Sjp_0302950   Nucleotide binding protein 1, putative
Schistosoma japonicum Sjp_0089600   Nucleotide-binding protein 2, putative
Schistosoma mansoni Smp_165770   nucleotide-binding protein 1 (nbp 1)/nbp35
Schistosoma mansoni Smp_154750   nucleotide binding protein 2 (nbp 2)
Schmidtea mediterranea mk4.030023.00   Cytosolic Fe-S cluster assembly factor NUBP1 homolog
Schmidtea mediterranea mk4.012841.00   Cytosolic Fe-S cluster assembly factor NUBP1 homolog
Schmidtea mediterranea mk4.002482.03   Iron-sulfur protein NUBPL
Schmidtea mediterranea mk4.004486.01  
Schmidtea mediterranea mk4.011732.00   Putative nucleotide-binding protein 1
Schmidtea mediterranea mk4.017209.01   Cytosolic Fe-S cluster assembly factor NUBP2
Schmidtea mediterranea mk4.044940.01   Iron-sulfur protein NUBPL
Schmidtea mediterranea mk4.001377.06   Putative nucleotide binding protein 2
Trypanosoma brucei gambiense Tbg972.10.2010   nucleotide binding protein, putative
Trypanosoma brucei gambiense Tbg972.11.13160   MRP protein, putative
Trypanosoma brucei gambiense Tbg972.7.1500   nucleotide binding protein, putative
Trypanosoma brucei Tb927.11.11730   MRP protein, putative
Trypanosoma brucei Tb927.10.1690   cytosolic Fe-S cluster assembly factor NBP35, putative
Trypanosoma brucei Tb927.7.1500   Cytosolic Fe-S cluster assembly factor CFD1, putative
Trypanosoma congolense TcIL3000.11.12390   MRP protein, putative
Trypanosoma congolense TcIL3000_10_1430   nucleotide binding protein, putative
Trypanosoma congolense TcIL3000_7_1010   nucleotide binding protein, putative
Trypanosoma cruzi TcCLB.511911.150   nucleotide-binding protein, putative
Trypanosoma cruzi TcCLB.507517.90   nucleotide-binding protein, putative
Trypanosoma cruzi TcCLB.508637.110   nucleotide-binding protein, putative
Trypanosoma cruzi TcCLB.511209.60   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.503579.100   nucleotide-binding protein, putative
Trypanosoma cruzi TcCLB.506025.10   hypothetical protein, conserved
Toxoplasma gondii TGME49_325500   ATP-binding Mrp/Nbp35 family protein
Toxoplasma gondii TGME49_318590   MRP family domain-containing protein
Toxoplasma gondii TGME49_280730   cytosolic fe-s cluster assembling factor nbp35, putative
Treponema pallidum TP0853   ATP-binding protein (ylxH-2)
Theileria parva TP03_0425   nucleotide binding protein, putative
Theileria parva TP03_0848   hypothetical protein, conserved
Trichomonas vaginalis TVAG_277380   nucleotide-binding protein, putative
Trichomonas vaginalis TVAG_076230   nucleotide binding protein, putative
Trichomonas vaginalis TVAG_157490   nucleotide binding protein, putative
Trichomonas vaginalis TVAG_178350   nucleotide binding protein, putative
Wolbachia endosymbiont of Brugia malayi Wbm0063   chromosome partitioning ATPase

Essentiality

TcCLB.503579.100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1250 Mycobacterium tuberculosis essential nmpdr
Tb11.01.3600 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.3600 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.3600 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.3600 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.7.1500 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.1500 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.1500 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.1500 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.1690 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.1690 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.1690 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.1690 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2113 Escherichia coli non-essential goodall
CELE_F10G8.6 Caenorhabditis elegans slow growth wormbase
YIL003W Saccharomyces cerevisiae inviable yeastgenome
YGL091C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0811900 Plasmodium berghei Essential plasmo
PBANKA_0919700 Plasmodium berghei Essential plasmo
TGME49_280730 Toxoplasma gondii Probably essential sidik
TGME49_318590 Toxoplasma gondii Probably essential sidik
TGME49_325500 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.503579.100 (Trypanosoma cruzi), nucleotide-binding protein, putative
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