Detailed view for NCLIV_057940

Basic information

TDR Targets ID: 481564
Neospora caninum, hypothetical protein
Source Database / ID: 

pI: 7.596 | Length (AA): 3141 | MW (Da): 332429 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 12 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
103 513 4tnd (A) 97 532 26.00 0 1 0.32225 0.26
152 444 3i6u (A) 183 488 32.00 0 1 0.456682 -0.06
157 444 3eqc (A) 39 363 24.00 0 1 0.359091 -0.3
163 504 4yhj (A) 160 524 27.00 0 1 0.375283 0.01
178 580 3lij (A) 51 471 27.00 0 1 0.441703 -0.06
182 454 4bfm (A) 7 280 40.00 0 1 0.456315 -0.12
183 458 2vz6 (A) 0 287 33.00 0 1 0.51777 -0.87
185 445 1phk (A) 15 290 37.00 0 1 0.539495 -0.89
730 870 2fz1 (B) 28 169 11.00 0 0 0.0927902 -0.2
779 956 4ke2 (A) 0 176 14.00 0 0.01 0.21897 -0.88
1203 1559 4yla (A) 5 355 16.00 0 0.85 0.134558 0.43
1791 1925 4qi3 (A) 48 182 11.00 0 0.01 0.13888 -0.46

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_139549)

Species Accession Gene Product
Cryptosporidium hominis Chro.60070   serine/threonine-protein kinase 2
Cryptosporidium parvum cgd6_520   Ser/Thr protein kinase
Neospora caninum NCLIV_057940   hypothetical protein
Plasmodium berghei PBANKA_1453400   protein kinase 2
Plasmodium falciparum PF3D7_1238900   protein kinase 2
Plasmodium knowlesi PKNH_1458700   serine/threonine protein kinase, putative
Plasmodium vivax PVX_100895   serine/threonine-protein kinase 2, putative
Plasmodium yoelii PY00790   probable serine/threonine-protein kinase 2
Toxoplasma gondii TGME49_315190   CAM kinase, SNF1 family

Essentiality

NCLIV_057940 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_315190 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus MAP kinase p38 alpha 360 aa 25.6% 317 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 28.1% 295 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 25.2% 314 aa Compounds References
Sus scrofa Casein kinase I isoform alpha 125 aa 30.9% 139 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 27.9% 283 aa Compounds References
Patiria pectinifera Cdc2 300 aa 29.3% 300 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 28.8% 292 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 32.7% 153 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 27.8% 284 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 30.9% 278 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier NCLIV_057940 (Neospora caninum), hypothetical protein
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