Detailed view for TcCLB.507887.20

Basic information

TDR Targets ID: 48855
Trypanosoma cruzi, ubiquitin hydrolase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.839 | Length (AA): 1515 | MW (Da): 169750 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00443   Ubiquitin carboxyl-terminal hydrolase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016579   protein deubiquitination  
GO:0036459   GO:thiol-dependent ubiquitinyl hydrolase activity  

GO:0005622   intracellular  
GO:0008270   zinc ion binding  
GO:0004221   ubiquitin thiolesterase activity  
GO:0006511   ubiquitin-dependent protein catabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
113 464 2gfo (A) 772 1106 28.00 0 1 0.31 -0.36
128 512 2f1z (A) 224 551 22.00 0 1 0.1 0.33
119 467 5cvm (A) 36 365 31.00 0 1 0.239463 0.31
119 523 5jtv (A) 215 893 28.00 0 1 0.215427 1.02
122 467 5k1a (A) 43 369 34.00 0.000024 0.74 -0.0525172 1.55
848 1029 4uos (A) 1 183 7.00 0.0015 0 0.255032 -1.23
1481 1512 2ebq (A) 3 34 34.00 0 0.55 0.357722 0.4
1481 1512 2d9g (A) 3 34 31.00 0 0.53 0.301722 0.64

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_146941)

Species Accession Gene Product
Leishmania braziliensis LbrM.16.0720   ubiquitin hydrolase, putative,cysteine peptidase, Clan CA, family C19, putative
Leishmania donovani LdBPK_160730.1   ubiquitin hydrolase, putative
Leishmania infantum LinJ.16.0730   ubiquitin hydrolase, putative,cysteine peptidase, Clan CA, family C19, putative
Leishmania major LmjF.16.0730   ubiquitin hydrolase, putative,cysteine peptidase, Clan CA, family C19, putative
Leishmania mexicana LmxM.16.0730   ubiquitin hydrolase, putative,cysteine peptidase, Clan CA, family C19, putative
Trypanosoma brucei gambiense Tbg972.8.5620   ubiquitin carboxyl-terminal hydrolase, putative
Trypanosoma brucei Tb927.8.5620   ubiquitin carboxyl-terminal hydrolase, putative
Trypanosoma congolense TcIL3000_0_47590   ubiquitin carboxyl-terminal hydrolase, putative
Trypanosoma cruzi TcCLB.506747.30   ubiquitin hydrolase, putative
Trypanosoma cruzi TcCLB.507887.20   ubiquitin hydrolase, putative

Essentiality

TcCLB.507887.20 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.5620 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5620 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.5620 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.5620 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania major ubiquitin hydrolase, putative,cysteine peptidase, Clan CA, family C19, putative Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Leishmania major ubiquitin hydrolase, putative,cysteine peptidase, Clan CA, family C19, putative 457 aa 23.0% 387 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0182 0.2574 0.2561
0.0278 0.2611 0.2611
0.0568 0.2558 0.2546
0.0234 0.2576 0.2788
0.0279 0.2712 0.2708
0.0271 0.2591 0.2537
0.0536 0.2558 0.2546
0.0278 0.2595 0.2595
0.0241 0.2595 0.2595
0.0004 0.5 0.5
0.0275 0.2595 0.2595
0.0244 0.2611 0.2611
0.0235 0.2523 0.2518
0.0253 0.2611 0.2611
0.028 0.2676 0.2672
0.0512 0.2558 0.2546
0.0262 0.2628 0.2628
0.0285 0.2698 0.2695
0.0278 0.2595 0.2595
0.0248 0.2595 0.2595
0.0266 0.2742 0.2726
0.0580791 0.331065 0.324488
0.0004 0.5 0.5
0.0272 0.2611 0.2611
0.0279 0.2712 0.2708
0.0243 0.2743 0.2726
0.0228 0.2691 0.2659
0.0281 0.2611 0.2611
0.0408 0.2558 0.2546
0.0244 0.2595 0.2595
0.0253 0.2611 0.2611
0.0238 0.2616 0.2611
0.0512 0.2558 0.2546
0.0256 0.2628 0.2628
0.0564 0.2558 0.2546
0.0580791 0.331065 0.324488
0.0262 0.2595 0.2595
0.0258 0.2595 0.2595
0.0236 0.2611 0.2611
0.0228 0.2611 0.2611
0.0234 0.2733 0.2726
0.028 0.262 0.2558
0.0279 0.2698 0.2695
0.0484 0.2558 0.2546
0.0285 0.2698 0.2696
0.0277 0.2698 0.2696
0.0247 0.2726 0.2726
0.0277 0.2698 0.2695
0.023 0.2616 0.2611
0.023 0.2525 0.2522
0.023 0.2616 0.2611
0.0568 0.2558 0.2546
0.023 0.2536 0.2534
0.023 0.2611 0.2611
0.0256 0.2628 0.2628
0.0234 0.2699 0.2696
0.0242 0.2595 0.2595
0.0258 0.2595 0.2595
0.0564 0.2558 0.2546
0.0127 0.2528 0.2528
0.0564 0.2575 0.2558
0.0235 0.2523 0.2518
0.0261 0.2742 0.2726
0.027 0.2644 0.2696
0.0239 0.2743 0.2726
0.0681888 0.330955 0.324488
0.0261 0.2681 0.2696
0.0184 0.2757 0.2752
0.0536 0.2558 0.2546
0.0236 0.2611 0.2611
0.0568 0.2558 0.2546
0.0244 0.2611 0.2611
0.0003 0.5 0.5
0.0245 0.2743 0.2726
0.027 0.2644 0.2696
0.028 0.2742 0.2726
0.0279 0.2712 0.2708
0.0236 0.2611 0.2611
0.0251 0.2743 0.2726
0.0228 0.2611 0.2611
0.0184 0.2622 0.2622
0.0004 0.5 0.5
0.028 0.2676 0.2672
0.0267 0.2611 0.2611
0.027 0.2702 0.2737
0.0234 0.2699 0.2696
0.0254 0.2536 0.2534
0.0184 0.2622 0.2622
0.0256 0.2528 0.2543
0.0282 0.2698 0.2696
0.0236 0.2733 0.2726
0.0353854 0.305123 0.305123
0.0258 0.2611 0.2611
0.0536 0.2558 0.2546
0.0248 0.2733 0.2726
0.0508 0.2558 0.2546
0.0236 0.2611 0.2611
0.025 0.2528 0.2534
0.0564 0.2546 0.2546
0.0256 0.2628 0.2628
0.0236 0.2611 0.2611
0.054 0.2558 0.2546
0.0273 0.2631 0.2727
0.0279 0.2698 0.2695
0.054 0.2558 0.2546
0.0255 0.2536 0.2534
0.0254 0.2528 0.2528
0.0508 0.2558 0.2546
0.057 0.2575 0.2557
0.0278 0.2595 0.2595
0.0512 0.2558 0.2546
0.0238 0.2595 0.2595
0.0232 0.2611 0.2611
0.028 0.2676 0.2672
0.0484 0.2558 0.2546
0.0241 0.2595 0.2595
0.054 0.2558 0.2546
0.0564 0.2575 0.2558
0.0254 0.2521 0.2493
0.0236 0.2733 0.2726
0.0536 0.2558 0.2546

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.507887.20 (Trypanosoma cruzi), ubiquitin hydrolase, putative
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